Insect Infection Model for<i>Campylobacter jejuni</i>Reveals That<i>O</i>‐methyl Phosphoramidate Has Insecticidal Activity

Champion, Olivia L.; Karlyshev, Andrey V.; Senior, Nicola J.; Woodward, Martin J.; Ragione, Roberto M. La; Howard, Sarah L.; Wren, Brendan W.; Titball, Richard W.

doi:10.1086/650494

Cited by 61 publications

(82 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…End points in G. mellonella infection models include survival rate, which can be assessed up to 5 d post infection, or longer with some fungal pathogens, facilitating the calculation of a maximum half lethal dose (LD50); expression of antimicrobial proteins in response to infection; production of lactate dehydrogenase as a marker of cell damage and biophotonic imaging to measure proliferation of bioluminescent microorganisms responsible for larval infection. [33][34][35][36][37] A pathological scoring system was recently proposed by Loh et al 38 in which an assessment of larval mobility, cocoon formation, melanisation and survival was used to assess larval health.…”

Section: G Mellonella To Study Toxinsmentioning

confidence: 99%

Galleria mellonellaas a model host for microbiological and toxin research

2016

View full text Add to dashboard Cite

Mammals are widely used by microbiologists as a model host species to study infectious diseases of humans and domesticated livestock. These studies have been pivotal for our understanding of mechanisms of virulence and have allowed the development of diagnostics, pre-treatments and therapies for disease. However, over the past decade we have seen efforts to identify organisms which can be used as alternatives to mammals for these studies. The drivers for this are complex and multifactorial and include cost, ethical and scientific considerations. Galleria mellonella have been used as an alternative infection model since the 1980s and its utility for the study of bacterial disease and antimicrobial discovery was recently comprehensively reviewed. The wider applications of G. mellonella as a model host, including its susceptibility to 29 species of fungi, 7 viruses, 1 species of parasite and 16 biological toxins, are described in this perspective. In addition, the latest developments in the standardisation of G. mellonella larvae for research purposes has been reviewed.

show abstract

Section: G Mellonella To Study Toxinsmentioning

confidence: 99%

Galleria mellonellaas a model host for microbiological and toxin research

2016

View full text Add to dashboard Cite

show abstract

“…Therefore, alternative infection models using insects are being increasingly employed to characterize virulence of bacterial pathogens and to evaluate novel therapeutics, prior to characterization in mammalian models (Glavis-Bloom et al, 2012). Larvae of the greater wax moth, Galleria mellonella, have been reported as a model for several intracellular bacteria, such as Burkholderia pseudomallei and Campylobacter jejuni, and also for Francisella tularensis and Legionella pneumophila, which are closely related to C. burnetii (Aperis et al, 2007;Schell et al, 2008;Champion et al, 2010;Wand et al 2011;Harding et al, 2012). G. mellonella can be maintained at mammalian body temperature (37 u C), are cheap, do not require feeding and are simple to manipulate at high containment.…”

Section: Introductionmentioning

confidence: 99%

Galleria mellonella as an alternative model of Coxiella burnetii infection

et al. 2014

View full text Add to dashboard Cite

Coxiella burnetii is a Gram-negative intracellular bacterium and is the causative agent of the zoonotic disease Q fever. Several rodent and non-human primate models of virulent phase I C. burnetii [Nine Mile (NM)I] have been developed, and have been used to determine the efficacy of antibiotics and vaccine candidates. However, there are several advantages to using insect models to study hostmicrobe interactions, such as reduced animal use, lowered cost and ease of manipulation in high containment. In addition, many laboratories use the avirulent phase II C. burnetii clone (NMII) to study cellular interactions and identify novel virulence determinants using genetic manipulation. We report that larvae of the greater wax moth, Galleria mellonella, were susceptible to infection with both C. burnetii NMI and NMII. Following subcutaneous infection, we report that intracellular bacteria were present within haemocytes and that larval death occurred in a dose-dependent manner. Additionally, we have used the model to characterize the role of the type 4 secretion system in C. burnetii NMII and to determine antibiotic efficacy in a non-mammalian model of disease.

show abstract

“…In addition to D. melanogaster, the larva of the greater wax moth Galleria mellonella has become a widely adopted insect model to study a wide range of human pathogens including Listeria spp. (23), Streptococcus pyogenes (36), Campylobacter jejuni (10), Yersinia pseudotuberculosis (9), and several pathogenic fungi (17,33). G. mellonella larvae can be easily maintained and infected by injection without anesthesia and sustain incubation at 37°C (33).…”

mentioning

confidence: 99%

Legionella pneumophila Pathogenesis in the Galleria mellonella Infection Model

et al. 2012

View full text Add to dashboard Cite

bLegionella pneumophila is a facultative intracellular human pathogen and the etiological agent of severe pneumonia known as Legionnaires' disease. Its virulence depends on protein secretion systems, in particular, the Dot/Icm type IV secretion system (T4SS), which is essential to establish a replication-permissive vacuole in macrophages. The analysis of the role of these systems and their substrates for pathogenesis requires easy-to-use models which approximate human infection. We examined the effectiveness of the larvae of the wax moth Galleria mellonella as a new model for L. pneumophila infection. We found that the L. pneumophila strains 130b, Paris, and JR32 caused mortality of the G. mellonella larvae that was strain, infectious dose, growth phase, and T4SS dependent. Wild-type L. pneumophila persisted and replicated within the larvae, whereas T4SS mutants were rapidly cleared. L. pneumophila strain Lp02, which is attenuated in the absence of thymidine but has a functional T4SS, resisted clearance in G. mellonella up to 18 h postinfection without inducing mortality. Immunofluorescence and transmission electron microscopy revealed that L. pneumophila resided within insect hemocytes in a vacuole that ultrastructurally resembled the Legionella-containing vacuole (LCV) observed in macrophages. The vacuole was decorated with the T4SS effector and LCV marker SidC. Infection caused severe damage to the insect organs and triggered immune responses, including activation of the phenoloxidase cascade leading to melanization, nodule formation, and upregulation of antimicrobial peptides. Taken together, these results suggest that G. mellonella provides an effective model to investigate the interaction between L. pneumophila and the host.

show abstract

Insect Infection Model forCampylobacter jejuniReveals ThatO‐methyl Phosphoramidate Has Insecticidal Activity

Cited by 61 publications

References 43 publications

Galleria mellonellaas a model host for microbiological and toxin research

Galleria mellonellaas a model host for microbiological and toxin research

Galleria mellonella as an alternative model of Coxiella burnetii infection

Legionella pneumophila Pathogenesis in the Galleria mellonella Infection Model

Contact Info

Product

Resources

About