Insect proteins as a potential source of antimicrobial peptides in livestock production. A review

Józefiak, Agata; Engberg, Ricarda M.

doi:10.22358/jafs/69998/2017

Cited by 139 publications

(130 citation statements)

References 82 publications

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“…In poultry, all segments of the GIT are colonized by different populations of microbiota (Salanitro et al, 1978;Pan and Yu, 2014;Józefiak and Engberg, 2017). To date, more than 900 bacterial species have been identified, and it is well documented that the disturbance of microbial homeostasis can have significant negative effects on intestinal health and bird performance (Stanley et al, 2014).…”

Section: Discussionmentioning

confidence: 99%

“…It is well-documented that insects produce AMPs that have broad-spectrum activity against both Gram-positive and Gram-negative bacteria, including Staphylococcus aureus (MRSA), Klebsiella pneumoniae, Shigella sonnei, Pseudomonas aeruginosa and Escherichia coli, and fungi, viruses and parasites (Park et al, 2014;Yi et al, 2014;Józefiak and Engberg, 2017). Additionally, the chitin content in insect meals could activate the innate immune system because of the fungistatic and immunoadjuvant properties (Dutta et al, 2004).…”

Section: Discussionmentioning

confidence: 99%

“…Insects are also known as a potential source of antimicrobial peptides (AMP) with activities against both Gram-positive and Gram-negative bacteria, which may be exploited in livestock production (Józefiak and Engberg, 2017). AMPs are considered growth and health promoters with modulating effects on the intestinal microbiome and do not result in the development of natural bacterial resistance (Wen and He, 2012;Choi, 2013a,b;Xiao, 2015;Józefiak and Engberg, 2017).…”

Section: Introductionmentioning

confidence: 99%

“…AMPs are considered growth and health promoters with modulating effects on the intestinal microbiome and do not result in the development of natural bacterial resistance (Wen and He, 2012;Choi, 2013a,b;Xiao, 2015;Józefiak and Engberg, 2017). Therefore, the aim of the present study was to examine the effect of the application of full-fat insect meals as a feed additive (0.05-0.2%) 'on top' of complete diets on the growth performance and GIT microbiota composition of broiler chickens.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Full-fat insect meals as feed additive – the effect on broiler chicken growth performance and gastrointestinal tract microbiota

Józefiak¹,

Kierończyk²,

Rawski³

et al. 2018

J. Anim. Feed Sci.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Full-fat insect meals as feed additive – the effect on broiler chicken growth performance and gastrointestinal tract microbiota

Józefiak¹,

Kierończyk²,

Rawski³

et al. 2018

J. Anim. Feed Sci.

Self Cite

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“…These AMPs have been considered as potential alternatives to the ‘classical antibiotics’ that leads to natural bacterial resistance. Especially, the insect AMPs have been supplemented in many types of human foodstuffs as preservative and livestock diets as an antibacterial additive (Jozefiak and Engberg ; Kieronczyk et al . ).…”

Section: Discussionmentioning

confidence: 99%

In silico identification, characterization and expression analysis of attacin gene family in response to bacterial and fungal pathogens in Tenebrio molitor

Park

et al. 2018

Entomological Research

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Antimicrobial peptides are effector molecules induced after microbial challenges. These form important components of innate host defense against the pathogens by exhibiting wide-spectrum antimicrobial activities. In this study, we identified three attacin-like genes from Tenebrio molitor RNASeq database using Tribolium castaneum attacin gene family as query. The T. molitor attacin gene family was annotated as TmAttacin-1a [comprising of 154 amino acids (aa)], TmAttacin-1b (150 aa) and TmAttacin-2 (164 aa), respectively. Temporal expression analysis shows that the TmAttacin-1a and -1b mRNAs are highly expressed in late larval stages, followed by a general decline in the pre-pupal stages. The mRNA level shows a decline during metamorphosis, and gets slightly overexpressed in pupal-adult transition stages. On the other hand, TmAttacin-2 is mainly overexpressed at 1-day old pupal stage. Spatial expression analysis indicates that TmAttacin-1a, À1b, and À2 mRNAs are primarily expressed in gut and fat body, but not in hemocytes and Malpighian tubules in T. molitor larvae. Interestingly, TmAttacin-1b shows more than 20-fold expression in the ovary, whereas TmAttacin-1a and À2 show similar expression patterns in gut, fat body, hemocyte, ovary, and testis in T. molitor adults. Induction pattern analysis demonstrates that the intracellular Gram-positive bacteria, L. monocytogenes elicited the strongest response by inducing~1,000-fold expression of TmAttacin-1a mRNA. The highest level of TmAttacin-1b mRNA (~350-fold) was induced by Gram-negative bacteria, E. coli. However, the TmAttacin-2 transcripts were not induced by microbial challenges. These results indicate that TmAttacin-1a and -1b may be required for antimicrobial defenses in T. molitor.

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Identification of a novel proline‐rich antimicrobial protein from the hemolymph of Antheraea mylitta

Chowdhury

Mandal

Dutta

et al. 2021

Arch Insect Biochem Physiol

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Antimicrobial proteins (AMPs) are small, cationic proteins that exhibit activity against bacteria, viruses, parasites, fungi as well as boost host‐specific innate immune responses. Insects produce these AMPs in the fat body and hemocytes, and release them into the hemolymph upon microbial infection. Hemolymph was collected from the bacterially immunized fifth instar larvae of tasar silkworm, Antheraea mylitta, and an AMP was purified by organic solvent extraction followed by size exclusion and reverse‐phase high‐pressure liquid chromatography. The purity of AMP was confirmed by thin‐layer chromatography and sodium dodecyl sulfate‐polyacrylamide gel electrophoresis analysis. The molecular mass was determined by matrix‐assisted laser desorption ionization–time of flight mass spectrometry as 14 kDa, and hence designated as AmAMP14. Peptide mass fingerprinting of trypsin‐digested AmAMP14 followed by de novo sequencing of one peptide fragment by tandem mass spectrometry analysis revealed the amino acid sequences as CTSPKQCLPPCK. No homology was found in the database search and indicates it as a novel AMP. The minimum inhibitory concentration of the purified AmAMP14 was determined against Escherichia coli, Staphylococcus aureus, and Candida albicans as 30, 60, and 30 µg/ml, respectively. Electron microscopic examination of the AmAMP14‐treated cells revealed membrane damage and release of cytoplasmic contents. All these results suggest the production of a novel 14 kDa AMP in the hemolymph of A. mylitta to provide defense against microbial infection.

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Insect proteins as a potential source of antimicrobial peptides in livestock production. A review

Cited by 139 publications

References 82 publications

Full-fat insect meals as feed additive – the effect on broiler chicken growth performance and gastrointestinal tract microbiota

Full-fat insect meals as feed additive – the effect on broiler chicken growth performance and gastrointestinal tract microbiota

In silico identification, characterization and expression analysis of attacin gene family in response to bacterial and fungal pathogens in Tenebrio molitor

Identification of a novel proline‐rich antimicrobial protein from the hemolymph of Antheraea mylitta

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