Insect sex-communication and prospects for pheromones in pest management

Piccardi, P.

doi:10.1080/11250008009438696

Cited by 6 publications

(4 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In contrast, for B. musae females, 15 compounds were identified in rectal gland extracts obtained from flies collected in both 2014 and 2017 (Table 1). Identified compounds included 13 saturated/unsaturated fatty acid esters, including ethyl caprate (5), methyl laurate (6), ethyl laurate (7), ethyl tridecanaote (8), propyl laurate (9), methyl myristate (10), ethyl myristate (11), ethyl myristoleate (12), methyl palmitate (13), ethyl palmitate (14), ethyl palmitoleate (15), ethyl oleate (16) and ethyl elaidate (17), as well as two spiroacetals (E,E)-2,8-dimethyl-1,7-dioxaspiro [5.5]undecane (3) and (E,E)-2-ethyl-8-methyl-1,7-dioxaspiro [5.5]undecane (4). Of these, ten compounds were also found in headspace collections.…”

Section: Assignment Of Compoundsmentioning

confidence: 99%

“…In tephritid fruit flies (Diptera: Tephritidae), courtship and mating are typically mediated by pheromones [1,2]. Sex pheromones are usually secreted and stored in the rectal glands and emitted during periods of mating activity [3][4][5][6]. The volatile compounds released by fruit flies are known to attract the opposite sex in many species [7][8][9][10], as well as members of the same sex to form mating aggregations [11,12].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Rectal Gland Chemistry, Volatile Emissions, and Antennal Responses of Male and Female Banana Fruit Fly, Bactrocera musae

Noushini

Pérez

Park

et al. 2019

Insects

View full text Add to dashboard Cite

The banana fruit fly, Bactrocera musae (Tryon) (Diptera: Tephritidae), is an economically important pest endemic to Australia and mainland Papua New Guinea. The chemistry of its rectal glands, and the volatiles emitted during periods of sexual activity, has not been previously reported. Using gas chromatography–mass spectrometry (GC-MS), we find that male rectal glands contain ethyl butanoate, N-(3-methylbutyl) acetamide, ethyl laurate and ethyl myristate, with ethyl butanoate as the major compound in both rectal gland and headspace volatile emissions. Female rectal glands contain four major compounds, ethyl laurate, ethyl myristate, ethyl palmitate and (E,E)-2,8-dimethyl-1,7-dioxaspiro[5.5]undecane, as well as 11 minor compounds. For both male and female B. musae, all compounds found in the headspace were also present in the rectal gland extracts, suggesting that the rectal gland is the main source of the headspace volatiles. Gas chromatography–electroantennography (GC-EAD) of rectal gland extracts confirms that male antennae respond to male-produced ethyl laurate and female-produced (E,E)-2,8-dimethyl-1,7-dioxaspiro[5.5]undecane, while female antennae respond to male-produced ethyl butanoate but no female-produced compounds. This is an important step in understanding the volatiles involved in the chemical communication of B. musae, their functional significance, and potential application.

show abstract

Section: Assignment Of Compoundsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Rectal Gland Chemistry, Volatile Emissions, and Antennal Responses of Male and Female Banana Fruit Fly, Bactrocera musae

Noushini

Pérez

Park

et al. 2019

Insects

View full text Add to dashboard Cite

show abstract

“…Because semiochemicals are commonly produced and released at low concentrations, efficient sampling methods are needed for collection and subsequent identification and quantification 1 – 5 . Tephritid fruit flies typically store pheromones in rectal glands and release them into the air during sexual activity 4 , 6 – 13 . Diverse sampling methods have been used to sample fruit fly pheromones and, in addition to genuine biological differences, some variation in pheromones reported for different fruit flies may actually arise from differences in the chemical collection efficiencies of the sampling methods used.…”

Section: Introductionmentioning

confidence: 99%

Sampling technique biases in the analysis of fruit fly volatiles: a case study of Queensland fruit fly

Noushini

Park

Jamie

et al. 2020

Sci Rep

View full text Add to dashboard Cite

Diverse methods have been used to sample insect semiochemicals. Sampling methods can differ in efficiency and affinity and this can introduce significant biases when interpreting biological patterns. We compare common methods used to sample tephritid fruit fly rectal gland volatiles (‘pheromones’), focusing on Queensland fruit fly, Bactrocera tryoni. Solvents of different polarity, n-hexane, dichloromethane and ethanol, were compared using intact and crushed glands. Polydimethylsiloxane, polydimethylsiloxane/divinylbenzene and polyacrylate were compared as adsorbents for solid phase microextraction. Tenax-GR and Porapak Q were compared as adsorbents for dynamic headspace sampling. Along with compounds previously reported for B. tryoni, we detected five previously unreported compounds in males, and three in females. Dichloromethane extracted more amides while there was no significant difference between the three solvents in extraction of spiroacetals except for (E,E)-2,8-dimethyl-1,7-dioxaspiro[5.5]undecane for which n-hexane extracted higher amount than both dichloromethane and ethanol. Ethanol failed to contain many of the more volatile compounds. Crushed rectal gland samples provided higher concentrations of extracted compounds than intact rectal gland samples, but no compounds were missed in intact samples. Of solid phase microextraction fibers, polyacrylate had low affinity for spiroacetals, ethyl isobutyrate and ethyl-2-methylbutanoate. Polydimethylsiloxane was more efficient for spiroacetals while type of fiber did not affect the amounts of amides and esters. In dynamic headspace sampling, Porapak was more efficient for ethyl isobutyrate and spiroacetals, while Tenax was more efficient for other esters and amides, and sampling time was a critical factor. Biases that can be introduced by sampling methods are important considerations when collecting and interpreting insect semiochemical profiles.

show abstract

“…The rectal gland is well known as a sex pheromonesecreting organ in fruit flies (Fletcher 1968(Fletcher , 1969Piccardi 1980;Perkins et al 1990a;Wee and Tan 2005;Tokushima et al 2010). The volatile compounds emitted during calling and courtship, especially by males, are known as short and long range attractants for the opposite sex in some species (Nation 1972;Perkins 1990;Sivinski et al 2000;Cruz-López et al 2015).…”

Section: Introductionmentioning

confidence: 99%

Rectal gland exudates and emissions of Bactrocera bryoniae: chemical identification, electrophysiological and pheromonal functions

et al. 2020

View full text Add to dashboard Cite

Bactrocera bryoniae is a polyphagous and economically significant fruit fly found in Indonesia, Papua New Guinea and Australia. To understand chemical-mediated sexual communication, and the potential for novel pheromone-based attractants for monitoring and mass-trapping of B. bryoniae, rectal gland exudates and emissions from sexually mature males and females were investigated. Gas chromatography-mass spectrometry showed that male rectal glands contained six compounds, of which 1,7-dioxaspiro [5,5]undecane elicited electroantennographic (EAD) and electropalpographic (EPD) responses in both sexes, ethyl 3-acetoxybutanoate elicited EPD responses in both sexes, N-(3-methylbutyl)acetamide elicited EAD response from males and 4-hydroxy-1,7-dioxaspiro[5.5]undecane elicited EAD responses in males and females and EPD responses in females. Female rectal glands contained 23 compounds with the esters ethyl laurate and ethyl myristate as major components. Amongst the female rectal gland constituents, ethyl laurate, ethyl myristate and ethyl palmitate elicited EAD responses in males and females, N-(3-methylbutyl)acetamide elicited EAD responses in males only, (E,E)-2,8-dimethyl-1,7-dioxaspiro[5.5]undecane elicited EAD responses in males and EPD responses in females, and 2,7-dimethyl-1,6-dioxaspiro[4.5] decane, (E,E)-2-ethyl-7-methyl-1,6-dioxaspiro[4.5]decane, (E,E)-2-ethyl-8-methyl-1,7-dioxaspiro[5.5]undecane, (Z,Z)-2,8-dimethyl-1,7-dioxaspiro[5.5]undecane, (E,E)-2-propyl-8-methyl-1,7-dioxaspiro[5.5]undecane and ethyl caprate elicited EPD responses in females only. Y-tube bioassays indicated that male rectal gland extracts and headspace volatiles attracted females and males, while female rectal gland extracts and headspace volatiles only attracted males. The results suggest that ethyl 3-acetoxybutanoate, 1,7-dioxaspiro [5,5]undecane and 4-hydroxy-1,7-dioxaspiro[5.5]undecane may be components of male-produced sex pheromone in B. bryoniae while (E,E)-2,8-dimethyl-1,7-dioxaspiro [5.5]undecane, N-(3-methylbutyl) acetamide, ethyl laurate, ethyl myristate and ethyl palmitate may be components of female-produced sex pheromone. Ethyl 3-acetoxybutanoate, N-(3-methylbutyl) acetamide, 1,7-dioxaspiro [5,5]undecane and 4-hydroxy-1,7-dioxaspiro[5.5]undecane may be components of male aggregation pheromone. These findings contribute to the understanding of pheromone communication in B. bryoniae and provide a foundation for developing pheromone-based monitoring and control methods.

show abstract

Insect sex-communication and prospects for pheromones in pest management

Cited by 6 publications

References 31 publications

Rectal Gland Chemistry, Volatile Emissions, and Antennal Responses of Male and Female Banana Fruit Fly, Bactrocera musae

Rectal Gland Chemistry, Volatile Emissions, and Antennal Responses of Male and Female Banana Fruit Fly, Bactrocera musae

Sampling technique biases in the analysis of fruit fly volatiles: a case study of Queensland fruit fly

Rectal gland exudates and emissions of Bactrocera bryoniae: chemical identification, electrophysiological and pheromonal functions

Contact Info

Product

Resources

About