Insecticidal Activity of Azadirachtin onDrosophila melanogasterand Recovery of Normal Status by Exogenous 20-Hydroxyecdysone

Boulahbel, Bilel; Aribi, Nadia; Kilani-Morakchi, Samira; Soltani, Noureddine

doi:10.4001/003.023.0104

Cited by 33 publications

(29 citation statements)

References 38 publications

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“…Azadirachtin acts with a dose–response relationship and the LD 50 we obtained (0.63 μg or 630 ppm , evaluated 24 h after treatment) is close to that found for D. melanogaster last larval instar also by topical application . Azadirachtin seems less toxic (1.17 μg) when applied on newly formed pupae of D. melanogaster . Neem oil, another azadirachtin formulation applied topically at the same stage, induces similar toxicity in Drosophila despite a different composition in the formulation.…”

Section: Discussionsupporting

confidence: 79%

“…The most likely explanation for this effect is a lower susceptibility due to the metabolism or penetration through the cuticle (puparium) at this stage of development. Nevertheless, the pupal stage, a critical phase for adult formation, remains very responsive to azadirachtin due to a drastic remodelling of most tissues and organs …”

Section: Discussionmentioning

confidence: 99%

“…Effects were assessed (1) following treatment on newly emerged adults, and (2) on various reproductive traits of adult males and females. Previous studies have already shown that azadirachtin impacts the reproduction of D. melanogaster treated at the larval and pupal stages . Thus, we considered its impact on the survival of progeny at various development stages and on fertility in both sexes.…”

Section: Introductionmentioning

confidence: 99%

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Azadirachtin effects on mating success, gametic abnormalities and progeny survival in Drosophila melanogaster (Diptera)

Oulhaci

Denis

Kilani-Morakchi

et al. 2017

Pest Management Science

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BACKGROUND: Azadirachtin is a prominent natural pesticide and represents an alternative to conventional insecticides. It has been successfully used against insect pests. However, its effects on reproduction require further analysis. Here we investigated lethal and sublethal effects of azadirachtin, on treated adults in a model insect, Drosophila melanogaster (Meigen). Dose-mortality relationships as well as several parameters of reproduction (mating, spermatogenesis, oogenesis and fertility) were examined.RESULTS: Neem-Azal, a commercial formulation of azadirachtin, applied topically on newly emerged adults, increased mortality with a positive dose-dependent relationship. The LD 50 (0.63 g) was determined 24 h after treatment using a non-linear regression. Adults surviving this dose had a mating success that was divided by 3 and a progeny production reduced by half when males were treated, and even more when females were treated. When combining probability of survival, of mating and reduced progeny, it appeared that LD 50 induced a 98% reduction in reproductive rates. Reduced progeny was partially explained by the effect of adult treatment on gametes number and abnormalities. The number of cysts and the apical nuclei positions within the cysts decreased by 29.7% and 20%, respectively, in males. In females, the number of oocytes per ovary and the volume of basal oocytes also decreased by 16.1% and 32.4%, respectively. CONCLUSION: Azadirachtin causes significant toxic effects in both sexes and decreases the fecundity and fertility of D. melanogaster. Females are more sensitive to azadirachtin.

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Section: Discussionsupporting

confidence: 79%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Azadirachtin effects on mating success, gametic abnormalities and progeny survival in Drosophila melanogaster (Diptera)

Oulhaci

Denis

Kilani-Morakchi

et al. 2017

Pest Management Science

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“…At present, a variety of plant‐derived pesticides has been approved for registration in China, where more than 50 pesticide companies are producing botanical pesticides, with more than 200 products being produced in total. Matrine was registered and produced as an active ingredient in most of the botanical pesticides, followed by azadirachtin . The main mode of action of botanical pesticides includes stomach poisoning, contact toxicity, anti‐feeding, inhibition of growth and development, anaesthesia and infertility, which is achieved by affecting the metabolism of insect hormones, the nervous system, respiratory system, muscle system, digestive system and reproductive system .…”

Section: Introductionmentioning

confidence: 99%

Laboratory and field evaluation of the aphidicidal activity of moso bamboo (Phyllostachys pubescens) leaf extract and identification of the active components

Gao

Shi

Liao

et al. 2019

Pest Management Science

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BACKGROUND Botanical pesticides increasingly play important roles in the control of agricultural pests. In this study, the aphidicidal effect of moso bamboo (Phyllostachys pubescens) extract against mustard aphid was confirmed, the main active compounds identified, and aphidicidal mechanism of the most active compound established. RESULTS When the treatment concentration was 10.0 g L−1, the corrected mortality of bamboo leaf extract (BE) was 53.22 ± 5.20% and the petroleum ether component of bamboo leaf extract (PE) reached 82.76 ± 4.50%, which also showed a synergistic effect with imidacloprid. Four flavonoids were identified as the main active components in the BE via activity tracking and phytochemical method. Isoorientin had an LC50 of 313.22 mg L−1, and affected the activities of acetylcholinesterase and peroxidase significantly, revealing the possible aphidicidal mechanism. When the treatment of 11.1% PE·imidacloprid was 200 mL, the control effect was 99.07%, which was better than that observed with 10% of imidacloprid or 0.5% of matrine. CONCLUSIONS These data provide a better understanding of the aphidicidal activity and aphidicidal mechanism of moso bamboo leaf extract and the most active compound, isoorientin. This will help in developing a more effective botanical aphicide. © 2019 Society of Chemical Industry

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“…Bioinsecticides are safer than synthetic pesticides due to rapid degradation in the environment and low toxicity to vertebrates (DERE et al, 2015). Therefore, one of the alternatives is the use of botanical insecticides, Azadirachtin A, is a triterpenoid belonging to the class limonoids, which is present mainly in the seeds of the neem tree (Azadirachta indica) (MORGAN 2009) and is one of the most biologically natural insecticides SciForum MOL2NET, 2017, 3, 10.3390/mol2net-03-05054 2 http://sciforum.net/conference/mol2net-03/wrsamc active (BOULAHBEL et al, 2015). Recently this compound was evaluated as a significant biopesticide and used for increasingly in pest control programs (BAJWA and AHMAD, 2012).…”

Section: Introductionmentioning

confidence: 99%

Molecular docking study of triterpenoid azadirachtin A on acetylcholinesterase of Drosophila melanogaster (Diptera: Drosophilidae)

Rodrigues

Scotti

2017

Proceedings of MOL2NET 2017, International Conference on Multidisciplinary Sciences, 3rd Edition

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Organic molecules of botanical origin can offer a source of compounds of pest management that are more environmentally acceptable and an efficient alternative to replace persistent synthetic insecticides. The molecular docking study using Molegro Virtual Docker software identified that the triterpenoid azadirachtin A showed stable conformations, with lower energy in the ligand-receptor complex of the compounds analyzed in this study, thus having a high affinity for the active site of the enzyme acetylcholinesterase, from a variety of interactions, which can determine its insecticidal potential against the species Drosophila melanogaster.

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Insecticidal Activity of Azadirachtin onDrosophila melanogasterand Recovery of Normal Status by Exogenous 20-Hydroxyecdysone

Cited by 33 publications

References 38 publications

Azadirachtin effects on mating success, gametic abnormalities and progeny survival in Drosophila melanogaster (Diptera)

Azadirachtin effects on mating success, gametic abnormalities and progeny survival in Drosophila melanogaster (Diptera)

Laboratory and field evaluation of the aphidicidal activity of moso bamboo (Phyllostachys pubescens) leaf extract and identification of the active components

<strong>Molecular docking study of triterpenoid azadirachtin A </strong><strong>on acetylcholinesterase of Drosophila melanogaster (Diptera: Drosophilidae)</strong>

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