Insecticidal activity of venomous saliva from Rhynocoris fuscipes (Reduviidae) against Spodoptera litura and Helicoverpa armigera by microinjection and oral administration

Sahayaraj, K.; Vinothkanna, A

doi:10.1590/s1678-91992011000400016

Cited by 9 publications

(5 citation statements)

References 17 publications

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“…Because reduviids can only ingest liquid food, the salivary secretions play a critical role in paralyzing and feeding of their prey. The toxic protein content of venomous saliva in reduviids varies with respect to males and females 38 . Records of adult female reduviids paralyzing their prey more rapidly than adult males reinstate the above fact 39 .…”

Section: Discussionmentioning

confidence: 99%

Temporal transcriptomic profiling of the ant-feeding assassin bug Acanthaspis cincticrus reveals a biased expression of genes associated with predation in nymphs

Kou

et al. 2017

Sci Rep

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Acanthaspis cincticrus (Stål) is an assassin bug with a specialized camouflaging behavior to ambush ants in the nymphal stages. In this study, we comprehensively sequenced all the life stages of A. cincticrus, including the eggs, five nymph instars, female and male adults using Illumina HiSeq technology. We obtained 176 million clean sequence reads. The assembled 84,055 unigenes were annotated and classified functionally based on protein databases. Among the unigenes, 29.03% were annotated by one or more databases, suggesting their well-conserved functions. Comparison of the gene expression profiles in the egg, nymph and adult stages revealed certain bias. Functional enrichment analysis of significantly differentially expressed genes (SDEGs) showed positive correlation with specific physiological processes within each stage, including venom, aggression, olfactory recognition as well as growth and development. Relative expression of ten SDEGs involved in predation process was validated using quantitative real-time PCR (qRT-PCR).

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Section: Discussionmentioning

confidence: 99%

Temporal transcriptomic profiling of the ant-feeding assassin bug Acanthaspis cincticrus reveals a biased expression of genes associated with predation in nymphs

Kou

et al. 2017

Sci Rep

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“…For CLC assemblies, reads with q-scores below 30 were trimmed and the reads assembled using minimum contig length of 150 bp, minimum similarity to join contig 0.95, and word (k-mer) sizes of 21,24,29,34,44,54, and 64. For Trinity, which employs a fixed k-mer method, reads were assembled using the default trimming parameters and minimum contig length of 150 bp.…”

Section: Methodsmentioning

confidence: 99%

Melt With This Kiss: Paralyzing and Liquefying Venom of The Assassin Bug Pristhesancus plagipennis (Hemiptera: Reduviidae)

Walker

Madio

Jin

et al. 2017

Molecular & Cellular Proteomics

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Assassin bugs (Hemiptera: Heteroptera: Reduviidae) are venomous insects, most of which prey on invertebrates. Assassin bug venom has features in common with venoms from other animals, such as paralyzing and lethal activity when injected, and a molecular composition that includes disulfide-rich peptide neurotoxins. Uniquely, this venom also has strong liquefying activity that has been hypothesized to facilitate feeding through the narrow channel of the proboscis-a structure inherited from sapand phloem-feeding phytophagous hemipterans and adapted during the evolution of Heteroptera into a fang and feeding structure. However, further understanding of the function of assassin bug venom is impeded by the lack of proteomic studies detailing its molecular composition.By using a combined transcriptomic/proteomic approach, we show that the venom proteome of the harpactorine assassin bug Pristhesancus plagipennis includes a complex suite of >100 proteins comprising disulfide-rich peptides, CUB domain proteins, cystatins, putative cytolytic toxins, triabin-like protein, odorant-binding protein, S1 proteases, catabolic enzymes, putative nutrient-binding proteins, plus eight families of proteins without homology to characterized proteins. S1 proteases, CUB domain proteins, putative cytolytic toxins, and other novel proteins in the 10 -16-kDa mass range, were the most abundant venom components. Thus, in addition to putative neurotoxins, assassin bug venom includes a high proportion of enzymatic and cytolytic venom components likely to be well suited to tissue liquefaction. Our results also provide insight into the trophic switch to blood-feeding by the kissing bugs (Reduviidae: Triatominae). Although some protein families such as triabins occur in the venoms of both predaceous and blood-feeding reduviids, the composition of venoms produced by these two groups is revealed to differ markedly. These results provide insights into the venom evolution in the insect suborder Heteroptera. Venoms are chemical arsenals injected by one animal into another to disrupt the homeostasis of the injected animal in ways that assist predation, defense, or feeding by the injecting animal (1). Typically, venoms are composed of multiple toxins, including peptides, enzymes, and small molecules, such as polyamines, that bind to and affect the function of multiple molecular targets in the injected animal. Because of their key role governing life-or-death interactions between animals, venom toxins are subject to selection pressures that have resulted in unique evolutionary patterns such as massive duplication and accelerated evolution of toxin-encoding genes (2-5). In addition, the properties that ensure that toxins confer a fitness advantage to the animals that produce them, including high stability and potency, make them well suited for use as insecticides, therapeutics, and pharmacological tools (6 -11). However, our understanding of the factors shaping venom evolution, and our ability to repurpose venom toxins for biotechnological use, is limited ...

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“…and peiratine ( Catamiarus brevipennis ) assassin bugs, but with doses that are likely substantially higher (2–9 μL of crude venom per gram of insect tissue) [ 46 , 111 , 140 , 143 ]. While Edwards [ 6 ] found that P. rhadamanthus venom had no effect when applied topically or orally to flies and cockroaches, both Sahayaraj and Muthukumar [ 143 ] and Sahayaraj and Vinothkanna [ 143 ] found that adding venom of Rhynocoris sp. diluted approximately 1:1000 to the feed of caterpillars over 96 h was sufficient to cause death.…”

Section: Diversification Of Venom Pharmacology In the Evolution Ofmentioning

confidence: 99%

Venoms of Heteropteran Insects: A Treasure Trove of Diverse Pharmacological Toolkits

Walker

Weirauch

Fry

et al. 2016

Toxins

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The piercing-sucking mouthparts of the true bugs (Insecta: Hemiptera: Heteroptera) have allowed diversification from a plant-feeding ancestor into a wide range of trophic strategies that include predation and blood-feeding. Crucial to the success of each of these strategies is the injection of venom. Here we review the current state of knowledge with regard to heteropteran venoms. Predaceous species produce venoms that induce rapid paralysis and liquefaction. These venoms are powerfully insecticidal, and may cause paralysis or death when injected into vertebrates. Disulfide-rich peptides, bioactive phospholipids, small molecules such as N,N-dimethylaniline and 1,2,5-trithiepane, and toxic enzymes such as phospholipase A2, have been reported in predatory venoms. However, the detailed composition and molecular targets of predatory venoms are largely unknown. In contrast, recent research into blood-feeding heteropterans has revealed the structure and function of many protein and non-protein components that facilitate acquisition of blood meals. Blood-feeding venoms lack paralytic or liquefying activity but instead are cocktails of pharmacological modulators that disable the host haemostatic systems simultaneously at multiple points. The multiple ways venom is used by heteropterans suggests that further study will reveal heteropteran venom components with a wide range of bioactivities that may be recruited for use as bioinsecticides, human therapeutics, and pharmacological tools.

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Insecticidal activity of venomous saliva from Rhynocoris fuscipes (Reduviidae) against Spodoptera litura and Helicoverpa armigera by microinjection and oral administration

Cited by 9 publications

References 17 publications

Temporal transcriptomic profiling of the ant-feeding assassin bug Acanthaspis cincticrus reveals a biased expression of genes associated with predation in nymphs

Temporal transcriptomic profiling of the ant-feeding assassin bug Acanthaspis cincticrus reveals a biased expression of genes associated with predation in nymphs

Melt With This Kiss: Paralyzing and Liquefying Venom of The Assassin Bug Pristhesancus plagipennis (Hemiptera: Reduviidae)

Venoms of Heteropteran Insects: A Treasure Trove of Diverse Pharmacological Toolkits

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