Insecticidal and genotoxic potential of two semi-synthetic derivatives of dillapiole for the control of Aedes (Stegomyia) aegypti (Diptera: Culicidae)

Domingos, Pedro Rauel Cândido; Pinto, Ana Cristina da Silva; Santos, Joselita Maria Mendes dos; Rafael, Míriam Silva

doi:10.1016/j.mrgentox.2014.07.008

Cited by 19 publications

(35 citation statements)

References 34 publications

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“…Other derivatives of dillapiole (ethyl ether dillapiole and butyl ether dillapiole) were seen to be effective in causing mortality in eggs and larvae of A. aegypti, besides causing chromosomal abnormalities, micronuclei formation and polynucleated cells (Domingos et al, 2014). In this species, the toxicity of isodillapiole caused changes in the relative expression levels of GSTE7 and CYP6N12 genes.…”

Section: Discussionmentioning

confidence: 99%

“…Rafael et al (2008) found that dillapiole caused cytogenotoxic effects such as polynucleated cells, micronuclei and chromosomal breaks in neuroblasts and oocytes of larvae in four successive generations of A. aegypti. In addition, semisynthetic derivatives of dillapiole (1KL39-B and 1KL43-C) showed cytogenotoxicity in A. aegypti, evidenced by the increase in mean frequency of nuclear anomalies in neuroblasts and oocytes in four generations (Domingos et al, 2014). These authors found cumulative effect of anomalies, affecting the fertility of the adult form of this mosquito.…”

Section: Introductionmentioning

confidence: 93%

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Effect of isodillapiole on the expression of the insecticide resistance genes GSTE7 and CYP6N12 in Aedes aegypti from central Amazonia

Lima¹,

Pinto²,

Rafael³

2015

Genet. Mol. Res.

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ABSTRACT. The yellow fever mosquito Aedes (Stegomyia) aegypti is the main vector of dengue arbovirus and other arboviruses. Dengue prevention measures for the control of A. aegypti involve mainly the use of synthetic insecticides. The constant use of insecticides has caused resistance in this mosquito. Alternative studies on plant extracts and their products have been conducted with the aim of controlling the spread of the mosquito. Dillapiole is a compound found in essential oils of the plant Piper aduncum (Piperaceae) which has been effective as a biopesticide against A. aegypti. Isodillapiole is a semisynthetic substance obtained by the isomerization of dillapiole. In the present study, isodillapiole was evaluated for its potential to induce differential expression of insecticide resistance genes (GSTE7 and CYP6N12) in 3rd instar larvae of A. aegypti. These larvae were exposed to 16729 Alternative of isodillapiole in the control of A. aegypti ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 14 (4): 16728-16735 (2015) this compound at two concentrations (20 and 40 µg/mL) for 4 h during four generations (G 1 , G 2 , G 3 , and G 4 ). Quantitative RT-PCR was used to assess the expression of GSTE7 and CYP6N12 genes. GSTE7 and CYP6N12 relative expression levels were higher at 20 than at 40 µg/mL and varied among generations. The decrease in GSTE7 and CYP6N12 expression levels at the highest isodillapiole concentration suggests that larvae may have suffered from metabolic stress, revealing a potential alternative product in the control of A. aegypti.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 93%

Effect of isodillapiole on the expression of the insecticide resistance genes GSTE7 and CYP6N12 in Aedes aegypti from central Amazonia

Lima¹,

Pinto²,

Rafael³

2015

Genet. Mol. Res.

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“…Dillapiole has toxic and genotoxic effects in the larvae and adult mosquitos of Ae. aegypti 8,13,14 and Ae. albopictus 15 .…”

Section: Introductionmentioning

confidence: 99%

Genotoxic Effects of Semi-Synthetic Isodillapiole on Oviposition in Aedes aegypti (Linnaeus, 1762) (Diptera: Culicidae)

Santos

Domingos

Meireles

et al. 2020

Rev. Soc. Bras. Med. Trop.

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INTRODUCTION: Semi-synthetic dillapiole compounds derived from Piper aduncum essential oil are used as alternative insecticides to control insecticide-resistant Aedes aegypti . Thus, we aimed to evaluate the genotoxic effects of semi-synthetic isodillapiole on the nuclei of neuroblasts (larvae) and oocytes (females) and the mean oviposition rates of the females over four generations (G 1 , G 2 , G 3 , and G 4 ) of Ae. aegypti . METHODS: Larvae were captured in the city of Manaus, Amazonas state, Brazil, and exposed to isodillapiole in bioassays (20, 40, and 60 µg/mL) and a negative control (0.05% DMSO in tap water) for 4 h. The cerebral ganglia were extracted from the larvae and oocytes from the adult females to prepare slides for cytogenetic analysis. Breeding pairs were established and eggs counts were quantified taken after the bioassays. RESULTS: The analysis of 20,000 interphase nuclei of neuroblasts and oocytes indicated significant genotoxicity (micronuclei, budding, polynucleated cells, and other malformations) compared to that of the control. Metaphasic and anaphasic nuclei presented chromosomal breaks; however, no significant variation and damage was observed in the negative control. A significant reduction in mean oviposition rates was also recorded following exposure to isodillapiole over the four generations (G 1 , G 2 , G 3 , and G 4 ). CONCLUSIONS: The toxic and genotoxic effects of isodillapiole on Ae. aegypti were caused by reduced oviposition in the females and nuclear abnormalities over the four generations of the trials. Further studies are required, rather than our in vitro assays, to verify the efficacy of exposure to this compound for controlling Ae. aegypti.

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“…Estas propriedades podem ser atribuídas ao fenilpropanoide dilapiol, componente de maior abundancia no óleo essencial desta espécie. Estudos com larvas de A. aegypti e A. albopictus mostraram o potencial de derivados semissintéticos do dilapiol como inseticida (DOMINGOS, et al, 2014;MEIRELES, et al, 2016;SILVA et al, 2019). Entretanto, dentre os diferentes derivados testados em larvas de A. aegypti e A. albopictus, o éter n-butil dilapiol (1KL43-C) apresentou melhor atividade inseticida (DOMINGOS, et al, 2014;MEIRELES, et al, 2016)…”

Section: Introductionunclassified

“…Costa et al (2010), estudaram o efeito tóxico de P. aduncum em larvas de A. aegypti e, registraram que a CL 50 foi de 30,19 μg/mL. Além desses autores, Santana et al (2015), demonstraram que o efeito do óleo essencial da P. aduncum em larvasde A. aegypti na CL 50 foi de 46 μg/mL e na CL 90 de 156 μg/mL Domingos et al (2014). analisaram os efeitos tóxicos do 1KL43-C, nas concentraçõesde 12,5, 20, 25, 30 e 40 μg/mL.…”

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Alicerces e Adversidades das Ciências da Saúde no Brasil 2

Malik¹,

Bös-Mikich²,

Aguiar³

et al. 2019

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Todo o conteúdo deste livro está licenciado sob uma Licença de Atribuição Creative Commons. Atribuição 4.0 Internacional (CC BY 4.0). O conteúdo dos artigos e seus dados em sua forma, correção e confiabilidade são de responsabilidade exclusiva dos autores. Permitido o download da obra e o compartilhamento desde que sejam atribuídos créditos aos autores, mas sem a possibilidade de alterá-la de nenhuma forma ou utilizá-la para fins comerciais.

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Insecticidal and genotoxic potential of two semi-synthetic derivatives of dillapiole for the control of Aedes (Stegomyia) aegypti (Diptera: Culicidae)

Cited by 19 publications

References 34 publications

Effect of isodillapiole on the expression of the insecticide resistance genes GSTE7 and CYP6N12 in Aedes aegypti from central Amazonia

Effect of isodillapiole on the expression of the insecticide resistance genes GSTE7 and CYP6N12 in Aedes aegypti from central Amazonia

Genotoxic Effects of Semi-Synthetic Isodillapiole on Oviposition in Aedes aegypti (Linnaeus, 1762) (Diptera: Culicidae)

Alicerces e Adversidades das Ciências da Saúde no Brasil 2

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