Insecticidal potential of an endophytic Cladosporium velox against Spodoptera litura mediated through inhibition of alpha glycosidases

Singh, Balbir; Kaur, Tamanreet; Kaur, Sanehdeep; Kaur, Amarjeet

doi:10.1016/j.pestbp.2016.01.004

Cited by 41 publications

(30 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Although the nematicidal activity of secondary compounds from Alternaria species has been explored (Lou et al 2016), our study is the first to illustrate the potential ecological significance of specific Alternaria strains on nematodes in planta using live plant assays, with both positive and negative effects on root-knot nematode galling. Similarly, some Cladosporium strains have been shown to produce secondary metabolites with nematicidal or insecticidal properties (Qureshi et al 2012;Singh et al 2016), but our results provide the first examples of negative in planta effects of multiple strains on nematodes. The genus Diaporthe (asexual state Phomopsis) includes endophytic species (Udayanga et al 2011) that can produce metabolites and have in planta effects that are deterrent to insect herbivory (Claydon et al 1985;McGee 2002), but we could find no prior examples of in planta effects on nematodes.…”

Section: Discussionmentioning

confidence: 48%

Tapping Into the Cotton Fungal Phytobiome for Novel Nematode Biological Control Tools

Zhou

Verma

Wheeler

et al. 2020

Phytobiomes Journal

View full text Add to dashboard Cite

A number of fungi have been shown to have negative effects on plant-parasitic nematodes. Most of these fungi have been isolated from soil, plant roots, or nematodes themselves. Fungi associated with crops can provide a diverse pool of candidates to test for antagonistic effects against plant parasites and other stressors. We used a hierarchical two-tiered approach to evaluate the efficacy and repeatability of 55 strains of fungi originally isolated as foliar facultative endophytes from upland cotton (Gossypium hirsutum) along with one commercial isolate of Beauveria bassiana for in planta antagonistic effects on root-knot nematodes (Meloidogyne incognita). All fungi were inoculated to cotton using a seed treatment. The number of root galls was quantified 3 weeks after egg inoculation of cotton seedlings. The majority of the fungi tested reduced the number of root galls relative to those on untreated control plants. To assess repeatability, 22 strains that exhibited the strongest reductions in gall numbers were further tested in replicate follow-up assays. Ninety-five percent (21/22) of these retested strains significantly reduced galling in the follow-up assay. Strains that reduced galling the most belonged to the genera Alternaria, Chaetomium, Cladosporium, Diaporthe, Epicoccum, Gibellulopsis, and Purpureocillium. On the contrary, three strains in the genera Alternaria and Curvularia significantly increased gall numbers. Our results indicate that a large proportion of the fungal strains originally isolated from cotton as naturally occurring foliar facultative endophytes are capable of reducing root-knot nematode infection when applied back to the plant as a seed treatment. These findings help establish a rich pool of candidate fungi for further evaluation as novel biological control tools against root-knot nematodes in cotton and other plants.

show abstract

Section: Discussionmentioning

confidence: 48%

Tapping Into the Cotton Fungal Phytobiome for Novel Nematode Biological Control Tools

Zhou

Verma

Wheeler

et al. 2020

Phytobiomes Journal

View full text Add to dashboard Cite

show abstract

“…Each compound was identified by its retention time and by spiking with standards under the same conditions (Singh et al . ).…”

Section: Methodsmentioning

confidence: 97%

“…Detection of phenolic compounds was carried out by comparison with standards. Each compound was identified by its retention time and by spiking with standards under the same conditions (Singh et al 2016).…”

Section: Phytochemical Analysismentioning

confidence: 99%

“…These fungal secondary metabolites are known to possess insecticidal and antifeedant activities (Amiri et al 1999;Kim et al 2002;Hafeez et al 2010). Although various studies have revealed the insecticidal potential of fungal spores, scant research has been done to investigate the potential of fungal secondary metabolites as biocontrol agents (Quesada-Moraga et al 2006;Abraham et al 2015;Singh et al 2016).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Evaluation of genotoxic and cytotoxic effects of ethyl acetate extract of Aspergillus flavus on Spodoptera litura

et al. 2019

View full text Add to dashboard Cite

show abstract

“…form tiny conidia in high amounts (Piecková and Jesenská, 1999), it can be concluded that this fungus is an efficient candidate for the biological control of arthropods. Singh et al (2016) described the potential of toxins produced by Cladosporium velox for exhibiting activities of Alpha glucosidase inhibitors, affecting the digestion and moulting of Spodoptera litura.…”

Section: Entomopathogenic Activity Of Cladosporium Cladosporioidesmentioning

confidence: 99%

Potential of a Cladosporium cladosporioides strain for the control of Tetranychus urticae Koch (Acari: Tetranychidae) under laboratory conditions

2019

View full text Add to dashboard Cite

The two spotted spider mite Tetranychus urticae is an important polyphagous pest worldwide. It is able to adapt to a wide variety of environments and has a high reproduction rate. In practice, farmers try to reduce losses by using synthetic acaricides. However, frequent and inadequate applications of acaricides have made this mite resistant to many active ingredients, creating the need to search for alternative control strategies. The aim of this research was to identify and to evaluate an indigenous strain of a fungus, which eliminated a T. urticae colony in a greenhouse. The isolated fungus was identified through a morphological and molecular characterization as Cladosporium cladosporioides. Thereafter, the mites were treated with five concentrations of C. cladosporioides conidia (2x104, 2x105, 2x106, 2x107, and 2x108 conidia ml-1) and a positive control (commercial Beauveria bassiana strain, 1x106 conidia ml-1). After 10 d, all treatments achieved at least 50% control; the concentrations 2x107 and 2x108 spores ml-1 controlled 73.3% and 81.7%, respectively, surpassing the commercial strain slightly (72.3%). TL50 ranged between 5 (2x108 spores ml-1) and 8 (2x104 spores ml-1) d, and LC50 was 1.95x106. The possible acaricidal effect of this strain on these mites is discussed.

show abstract

Insecticidal potential of an endophytic Cladosporium velox against Spodoptera litura mediated through inhibition of alpha glycosidases

Cited by 41 publications

References 22 publications

Tapping Into the Cotton Fungal Phytobiome for Novel Nematode Biological Control Tools

Tapping Into the Cotton Fungal Phytobiome for Novel Nematode Biological Control Tools

Evaluation of genotoxic and cytotoxic effects of ethyl acetate extract of Aspergillus flavus on Spodoptera litura

Potential of a Cladosporium cladosporioides strain for the control of Tetranychus urticae Koch (Acari: Tetranychidae) under laboratory conditions

Contact Info

Product

Resources

About