Insecticidal spider venom toxin fused to snowdrop lectin is toxic to the peach‐potato aphid, <i>Myzus persicae</i> (Hemiptera: Aphididae) and the rice brown planthopper, <i>Nilaparvata lugens</i> (Hemiptera: Delphacidae)

Down, Rachel E.; Fitches, Elaine; Wiles, Duncan Peter; Cortí, Paola; Bell, Howard A.; Gatehouse, John A.; Edwards, John

doi:10.1002/ps.1119

Cited by 72 publications

(44 citation statements)

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“…Sadeghi et al (2009a) reported an LC50 value for GNA of 350 µg mL -1 for pea aphids, which is about three times more than what is required in the current study to kill the same percentage of aphids. Down et al (2006) reported < 10% mortality of M. persicae after 14 d exposure to diet containing 1.4 g L -1 of GNA lectin. The response of each species of aphid to phytochemicals was quite different.…”

Section: Discussionmentioning

confidence: 99%

Insecticidal activity of a protein extracted from bulbs of Phycella australis Ravenna against the aphids Acyrthosiphon pisum Harris and Myzus persicae Sulzer

Zapata¹,

Damme

Vargas³

et al. 2016

Chilean J. Agric. Res.

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Aphids cause significant losses in many agricultural crops and in many cases cause repeated insecticide sprays, which increase the risk of resistance. Therefore, other alternatives are needed to control them. The toxic, antireproductive, and feeding deterrent effects of a mannosebinding lectin isolated from bulbs of Phycella australis Ravenna (Amaryllidaceae), named Phycella australisagglutinin (PAA) was assayed on nymphs of the aphids Acyrthosiphon pisum Harris and Myzus persicae Sulzer fed with an artificial diet. After 72 h of PAA exposure, lethal concentration (LC50) values were 109 and 313 µg mL -1 for A. pisum and M. persicae, respectively, while LC90 values were 248 and 634 µg mL -1 . Sub-lethal concentrations of PAA significantly reduced the aphid fecundity at a concentration of 80 µg mL -1 . Only a total of 5.7 descendants per female were recorded for A. pisum (32% control progeny) and 12.4 for M. persicae (39% control progeny). Acyrthosiphon pisum was strongly deterred by PAA under choice conditions, as after 72 h exposed to 80 µg PAA mL -1 of diet, the feeding deterrent index was 0.91 for A. pisum and only 0.38 for M. persicae. In conclusion, the mannosebinding lectin isolated from bulbs of P. australis showed acute and chronical insecticidal activity against the pea and green peach aphids.

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Section: Discussionmentioning

confidence: 99%

Insecticidal activity of a protein extracted from bulbs of Phycella australis Ravenna against the aphids Acyrthosiphon pisum Harris and Myzus persicae Sulzer

Zapata¹,

Damme

Vargas³

et al. 2016

Chilean J. Agric. Res.

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“…One of the proteins used is a lectin, which functions as a carrier (GNA, Galanthus nivalis agglutinin) and the other protein is a toxin. A fusion protein comprising GNA and SFI1 has been shown to have insecticidal effects on both lepidopteran and homopteran plant pests (Fitches et al, 2004;Down et al, 2006) while a fusion protein combining GNA with ButaIT has also been shown to have insecticidal activity against lepidopteran larvae. The injection data reported show that fusion proteins containing SFI1 and ButalT are insecticidal towards a range of insects including lepidopteran, dipteran, coleopteran and dictyopteran pests (Fitches et al, 2010).…”

Section: Future Prospectsmentioning

confidence: 99%

Baculoviruses: Members of Integrated Pest Management Strategies

Rodríguez¹,

Belaich²,

Ghiringhelli³

2012

Integrated Pest Management and Pest Control - Current and Future Tactics

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“…Consequently, GNA has been exploited as a transporter for several different insecticidal toxins (Fitches et al, 2001;Fitches et al, 2004;Down et al, 2006;Trung et al, 2006;Wakefield et al, 2006;Fitches et al, 2012). GNA itself is weakly insecticidal in some insect species, but not as potent as insecticidal spider toxins.…”

Section: Discussionmentioning

confidence: 99%

“…GNA has been used to improved the oral activity of a variety of insecticidal neurotoxins (Fitches et al, 1997;Fitches et al, 2001;Fitches et al, 2002;Fitches et al, 2004;Down et al, 2006;Trung et al, 2006;Wakefield et al, 2006;Fitches et al, 2010;Wakefield et al, 2010;Fitches et al, 2012). GNA and CP both bind to receptors in the insect gut and subsequently cross the epithelium by transcytosis (Fitches et al, 1997;Fitches et al, 2001;Fitches et al, 2012;Bonning and Chougule, 2014; .…”

Section: Discussionmentioning

confidence: 99%

“…However, the special ability of GNA to transcytose cross the midgut of insects has received considerable attention in past few years. GNA has been used to successfully deliver a number of venom-derived insecticidal peptide toxins, including Hv1a, into insect hemolymph (Fitches et al, 2002;Fitches et al, 2004;Down et al, 2006;Trung et al, 2006;Fitches et al, 2010;Wakefield et al, 2010;Fitches et al, 2012). Moreover, the oral activity of these insecticidal venom peptides was shown to be much higher when they were fused to GNA.…”

Section: Potential Of Insecticidal Arthropod-venom Peptides As Bioinsmentioning

confidence: 99%

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Engineering insect-resistant plants by transgenic expression of an insecticidal spider-venom peptide

Alam¹

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The insecticidal spider-venom peptide ω-hexatoxin-Hv1a (Hv1a) from the Australian Blue Mountains funnel-web spider Hadronyche versuta is one of the most potent insect-specific neurotoxins isolated to date. Hv1a blocks voltage-gated calcium channels in the insect central nervous system, a mechanism quite distinct from existing chemical insecticides. It induces a slow-onset paralysis that precedes death in a taxonomically wide range of insects. Hv1a's broad spectrum of target insects, novel mode of action, and absence of toxicity to vertebrates makes the Hv1a gene an attractive tool for generating insectresistant transgenic crops.The oral activity of Hv1a can be enhanced by coupling it to the plant lectin Galanthus nivalis agglutinin (GNA) or with the minor capsid protein of pea enation mosaic virus (CP).Recombinant fusions of Hv1a with GNA were produced using the Pichia pastoris expression system to study the intrinsic insecticidal activity of Hv1a-GNA and GNA-Hv1a fusion proteins. By using injection bioassays with houseflies, we found that the intrinsic insecticidal activity of Hv1a was maintained when it was fused to GNA. Moreover, feeding bioassays with diamondback moth larvae revealed that fusion of Hv1a to GNA, in either orientation, enhances its oral insecticidal activity.In order to generate transgenic plants expressing Hv1a alone or fused to GNA or CP, transformation vectors were constructed by ligating synthesised genes in the pAOV binary vector with the constitutive Cauliflower Mosaic Virus 35S promoter (35S) or the phloem tissue-specific Arabidopsis thaliana SUCROSE TRANSPORTER 2 (SUC2) promoter.Homozygous transgenic Arabidopsis were generated using the floral dip method of Agrobacterium-mediated plant transformation and subsequent herbicide selection. PCR of genomic DNA and western blotting were used to confirm integration of the transgenes and protein expression in the transgenic Arabidopsis respectively. Initial experiments revealed a very high level of mortality of Helicoverpa armigera larvae on wild-type plants due to the presence of endogenous glucosinolates, which masked the insecticidal effects of the transgenes Thus, a new set of transgenic plants was generated using an Arabidopsis cyp79B2 cyp79B myb28 myb29 quadruple mutant that lacks endogenous glucosinolates.Bioassays revealed that H. armigera larvae had a lower level of survival and retarded growth when fed on leaves of transgenic Arabidopsis expressing Hv1a toxins under 35S promoter control compared with those fed on gluc-null control plants. Moreover, larval mortality was higher for plants expressing Hv1a/GNA fusions than those expressing Hv1a or GNA alone. The highest larval mortality, lowest larval weight gain, and lowest level of leaf damage were observed for larvae fed on plants expressing GNA-Hv1a. Mortality was extremely high (~90%) for larvae fed on GNA-Hv1a plants for 15 days. The resistance to cotton bollworms conferred by expression of GNA-Hv1a in transgenic Arabidopsis highlights the potential of Hv1a transgenes as an ...

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Insecticidal spider venom toxin fused to snowdrop lectin is toxic to the peach‐potato aphid, Myzus persicae (Hemiptera: Aphididae) and the rice brown planthopper, Nilaparvata lugens (Hemiptera: Delphacidae)

Cited by 72 publications

References 36 publications

Insecticidal activity of a protein extracted from bulbs of Phycella australis Ravenna against the aphids Acyrthosiphon pisum Harris and Myzus persicae Sulzer

Insecticidal activity of a protein extracted from bulbs of Phycella australis Ravenna against the aphids Acyrthosiphon pisum Harris and Myzus persicae Sulzer

Baculoviruses: Members of Integrated Pest Management Strategies

Engineering insect-resistant plants by transgenic expression of an insecticidal spider-venom peptide

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