Colorimetric methods are described for determining Sevin and its metabolities, 1 -naphthol and 1 -naphthol conjugates, in poultry tissues and eggs. Free naphthol is reacted with pnitrobenzenediazonium fluoborate to produce a colored product which is measured spectrophotometrically. Sevin and 1 -naphthol conjugates are hydrolyzed to 1 -naphthol which is then determined in the same manner.Residue studies with poultry dusted with Sevin showed highest concentrations of residues to be in skin tissue where an average of 1 9.3 p.p.m. Sevin was found 1 day after the final treatment. A 7-day waiting period between treatment and slaughter reduced these residues to 2.15 p.p.m. No significant amount of either 1 -naphthol or its conjugates was found in any tissue of birds processed 7 days after treatment, and eggs were found free of residues throughout the study.
Sevin(1-naphthyl '-methylcarbamate), a broad spectrum insecticide developed by Union Carbide Chemicals Co., has proved highly effective for controlling chicken mites, northern fowl mites, lice, and other ectoparasites of poultry (7). Control is gained by treating the litter in the cages or by direct application of the insecticide to the birds.To study the extent of contamination that may result in the edible tissues of birds treated with Sevin, a dusting experiment was conducted jointly between the Research and Development Department of Union Carbide Chemicals Co. and the Zoology-Entomology Department of Auburn University. The experiment was designed to provide residue data in all types of edible tissue for both the parent insecticide and its metabolite, 1-naphthol. Each type of tissue, including skin, dark and white muscle, liver, and gizzard, was analyzed separately to show' the distribution of residues in the carcass.