Insecticide resistance in dengue vectors from hotspots in Selangor, Malaysia

Rasli, Rosilawati; Cheong, Young-Keun; Ibrahim, Mohd Khairuddin Che; Fikri, Siti Futri Farahininajua; Norzali, Rusydi Najmuddin; Nazarudin, Nur Ayuni; Hamdan, Nur Fadillah; Muhamed, Khairul Asuad; Hafisool, Afiq Ahnaf; Azmi, Ruziyatul Aznieda; Ismail, Harith Aswad; Ali, Roziah; Hamid, Nurulhusna Ab; Taib, Mohd Zainuldin; Omar, Topek; Ahmad, Nazni Wasi; Lee, Hanlim

doi:10.1371/journal.pntd.0009205

Cited by 15 publications

(6 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…When this point reached, such resistant mosquitoes were predicted to be survived. Likewise, similar results were also reported in Rasli et al (2021). Therefore, those populations characterised with high or moderate MFO enzyme activities with less or no impact of PBO along with insecticide pyrethroids, should be further investigated, especially on the significance of kdr gene in the development of insecticide resistance.…”

Section: Discussionsupporting

confidence: 77%

Bioefficacy of mosquito mat vaporizers and associated metabolic detoxication mechanisms in Aedes aegypti (Linnaeus) in Selangor, Malaysia: A statewide assessment

et al. 2021

View full text Add to dashboard Cite

This study aims to examine the efficacy of mosquito mat vaporizers on Aedes aegypti and their associated metabolic detoxication mechanisms. For this purpose, Aedes aegypti (Linnaeus) was collected from nine districts in Selangor, Malaysia and tested with mosquito vaporizing mat bioassays. The same populations were also subjected to biochemical assays to investigate activities of detoxifying enzymes, namely non-specific esterase (EST), glutathione-S-transferase (GST) and mixed function oxidase (MFO). The efficacy of Ae. aegypti on the active ingredients tested in decreasing order were d-allethrin > dimefluthrin > prallethrin with PBO > prallethrin. The results further indicated significant enhancement mean levels of EST, GST and MFO in pyrethroid-resistant populations. The mortality rate of Ae. aegypti in response to pyrethroid active ingredients was associated with MFO activity, suggesting it is an important detoxification enzyme for the populations tested. In view of the presence of resistance against household insecticide products, pyrethroid efficacy on Ae. aegypti populations needs to be monitored closely to ensure the implementation of an effective vector control program in Malaysia.

show abstract

Section: Discussionsupporting

confidence: 77%

Bioefficacy of mosquito mat vaporizers and associated metabolic detoxication mechanisms in Aedes aegypti (Linnaeus) in Selangor, Malaysia: A statewide assessment

et al. 2021

View full text Add to dashboard Cite

show abstract

“…On the other hand, recent studies conducted in dengue hotspot areas of Selangor emphasised possible risks to the success of insecticides presently implemented in dengue vector control. Rasli et al [ 18 ] studied the largest dengue hotspot areas, with about 24 different dengue hotspot areas. They showed high and significant insecticide-resistant to pyrethroid and organophosphate.…”

Section: Discussionmentioning

confidence: 99%

“…In Penang Island, Zuharah et al [ 19 ] reported the risk of developing insecticide resistance towards pyrethroid with around 87% mortality rates towards permethrin in the Northern district of Penang. Rasli et al [ 18 ] and Zuharah et al [ 19 ] indicated insecticide resistance in Ae . aegypti in the dengue hotspot areas similar to the current study.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Quantitative proteomics analysis of permethrin and temephos-resistant Ae. aegypti revealed diverse differentially expressed proteins associated with insecticide resistance from Penang Island, Malaysia

Shettima,

Ishak,

Lau

et al. 2023

PLoS Negl Trop Dis

View full text Add to dashboard Cite

Synthetic insecticides are the primary vector control method used globally. However, the widespread use of insecticides is a major cause of insecticide-resistance in mosquitoes. Hence, this study aimed at elucidating permethrin and temephos-resistant protein expression profiles in Ae. aegypti using quantitative proteomics. In this study, we evaluated the susceptibility of Ae. aegypti from Penang Island dengue hotspot and non-hotspot against 0.75% permethrin and 31.25 mg/l temephos using WHO bioassay method. Protein extracts from the mosquitoes were then analysed using LC–ESI–MS/MS for protein identification and quantification via label-free quantitative proteomics (LFQ). Next, Perseus 1.6.14.0 statistical software was used to perform differential protein expression analysis using ANOVA and Student’s t-test. The t-test selected proteins with≥2.0-fold change (FC) and ≥2 unique peptides for gene expression validation via qPCR. Finally, STRING software was used for functional ontology enrichment and protein-protein interactions (PPI). The WHO bioassay showed resistance with 28% and 53% mortalities in adult mosquitoes exposed to permethrin from the hotspot and non-hotspot areas. Meanwhile, the susceptibility of Ae. aegypti larvae revealed high resistance to temephos in hotspot and non-hotspot regions with 80% and 91% mortalities. The LFQ analyses revealed 501 and 557 (q-value <0.05) differentially expressed proteins in adults and larvae Ae. aegypti. The t-test showed 114 upregulated and 74 downregulated proteins in adult resistant versus laboratory strains exposed to permethrin. Meanwhile, 13 upregulated and 105 downregulated proteins were observed in larvae resistant versus laboratory strains exposed to temephos. The t-test revealed the upregulation of sodium/potassium-dependent ATPase β2 in adult permethrin resistant strain, H15 domain-containing protein, 60S ribosomal protein, and PB protein in larvae temephos resistant strain. The downregulation of troponin I, enolase phosphatase E1, glucosidase 2β was observed in adult permethrin resistant strain and tubulin β chain in larvae temephos resistant strain. Furthermore, the gene expression by qPCR revealed similar gene expression patterns in the above eight differentially expressed proteins. The PPI of differentially expressed proteins showed a p-value at <1.0 x 10−16 in permethrin and temephos resistant Ae. aegypti. Significantly enriched pathways in differentially expressed proteins revealed metabolic pathways, oxidative phosphorylation, carbon metabolism, biosynthesis of amino acids, glycolysis, and citrate cycle. In conclusion, this study has shown differentially expressed proteins and highlighted upregulated and downregulated proteins associated with insecticide resistance in Ae. aegypti. The validated differentially expressed proteins merit further investigation as a potential protein marker to monitor and predict insecticide resistance in field Ae. aegypti. The LC-MS/MS data were submitted into the MASSIVE database with identifier no: MSV000089259.

show abstract

“…Long-term use of temephos has given rise to resistant strains in Ae. aegypti mosquito populations in various countries, such as recent reports in Brazil 8 , Cuba 9 , Indonesia 10 , Malaysia 11 and Peru 12 , in addition to genotoxic effects in humans 13 .…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Larvicidal Activity of Kaempferia galanga Extracts Against Aedes aegypti Larvae

Anwar¹,

Hida²,

Sumanto³

et al. 2022

Pakistan J. of Biological Sciences

View full text Add to dashboard Cite

Background and Objective: The resistance of Aedes aegypti larvae against temephos become an obstacle in controlling the arboviral vector. This condition triggered researchers to investigate the larvicidal activity of phytochemical compounds that are effective, safe, biodegradable and eco-friendly from various medicinal plants. This study evaluated the larvicidal activity of Kaempferia galanga extracts against Ae. aegypti larvae. Materials and Methods: Four solvents with different polarities, namely ethanol, ethyl acetate, n-hexane and water were used in the sequential extraction. The final larvicidal bioassay test of the four extract types was designed in five replicates of five concentration ranges, namely 1.0, 8.75, 17.5, 35.0 and 70.0 ppm. A total of 20 3rd instar larvae of Ae. aegypti were contacted with each replicate in a plastic cup. Larval mortality and effective concentration of larvicide were calculated and determined after 24 and 48 hrs of exposure. Results: The average range of larval mortality according to the concentration of larvicide extracts of ethanol, ethyl

show abstract

Insecticide resistance in dengue vectors from hotspots in Selangor, Malaysia

Cited by 15 publications

References 16 publications

Bioefficacy of mosquito mat vaporizers and associated metabolic detoxication mechanisms in Aedes aegypti (Linnaeus) in Selangor, Malaysia: A statewide assessment

Bioefficacy of mosquito mat vaporizers and associated metabolic detoxication mechanisms in Aedes aegypti (Linnaeus) in Selangor, Malaysia: A statewide assessment

Quantitative proteomics analysis of permethrin and temephos-resistant Ae. aegypti revealed diverse differentially expressed proteins associated with insecticide resistance from Penang Island, Malaysia

Evaluation of Larvicidal Activity of Kaempferia galanga Extracts Against Aedes aegypti Larvae

Contact Info

Product

Resources

About