Insecticide resistance is mediated by multiple mechanisms in recently introduced Aedes aegypti from Madeira Island (Portugal)

Seixas, Gonçalo; Grigoraki, Linda; Weetman, David; Vicente, José L.; Silva, Ana Clara; Pinto, João; Vontas, John; Sousa, Carla A.

doi:10.1371/journal.pntd.0005799

Cited by 64 publications

(67 citation statements)

References 44 publications

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“…Coupled with possible resistance observed recently in Spain and the high levels of resistance found in the only Western European Ae. aegypti population from the island of Madeira, the results should serve as a warning for Europe and encourage further efforts in monitoring this phenomenon and in standardizing protocols for IR detection and guidelines for IR management in temperate areas. Studies of this typology are in fact greatly needed to support local public health authorities in managing and planning effective control measures and to maintain effective insecticide‐based vector control options.…”

Section: Discussionmentioning

confidence: 99%

First evidence of resistance to pyrethroid insecticides in ItalianAedes albopictuspopulations 26 years after invasion

Pichler

Bellini

Veronesi

et al. 2018

Pest Management Science

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This study reports the first evidence of resistance to pyrethroids in adult Italian Ae. albopictus populations. Results refer to the season preceding the Chikungunya 2017 outbreak in central Italy and highlight the need to increase efforts to monitor the spread of insecticide resistance and the need to develop strategies to limit the spread of insecticide resistance, particularly in areas where extensive treatments have been carried out to contain disease outbreaks. © 2017 Society of Chemical Industry.

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Section: Discussionmentioning

confidence: 99%

First evidence of resistance to pyrethroid insecticides in ItalianAedes albopictuspopulations 26 years after invasion

Pichler

Bellini

Veronesi

et al. 2018

Pest Management Science

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“…The V410L, S989P, V1016G/I and F1534C mutations are common, but vary geographically in A. aegypti . V410L has been detected in the Americas, and V1016I has been detected only in the Americas, Europe and Africa . In contrast, S989P has been found only in Asia .…”

Section: Introductionmentioning

confidence: 99%

“…V1016G mutations have been found in Asia and the Americas . F1534C is widely distributed in pyrethroid resistant A. aegypti globally . Nowadays, nearly every pyrethroid resistant A. aegypti population has alleles with the F1534C mutation, and in some resistant populations the F1534 allele is undetectable .…”

Section: Introductionmentioning

confidence: 99%

The F1534C voltage‐sensitive sodium channel mutation confers 7‐ to 16‐fold resistance to pyrethroid insecticides in Aedes aegypti

Fan

Scott

2020

Pest Management Science

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BACKGROUND: Recent outbreaks of dengue and Zika have emphasized the importance to effectively control Aedes aegypti, which vectors the viruses causing these diseases. Pyrethroid insecticides are primarily used to control adult A. aegypti, especially during disease outbreaks. However, pyrethroid resistance in A. aegypti is an increasing problem. Mutations in the voltage-sensitive sodium channel (Vssc) are a common mechanism of pyrethroid resistance. The F1534C mutation is common and distributed globally in A. aegypti populations, but previous studies disagree about the role of this mutation in conferring resistance to pyrethroid insecticides. RESULTS:We isolated a congenic strain (1534C:ROCK) which was closely related to a susceptible strain Rockefeller (ROCK), but was homozygous for the 1534C Vssc allele. We determined resistance levels against eight insecticides that target the VSSC: six pyrethroids, DDT and DCJW (the bioactivated metabolite of indoxacarb). The resistance levels ranged from 7-to 16-fold, and resistance was inherited as an incompletely recessive trait. We also found a novel 367I+1520I+1534C allele, in addition to the 1534C and 1520I+1534C alleles, in mosquitoes from Thailand. The T1520I mutation did not increase pyrethroid resistance beyond what was conferred by the F1534C mutation alone. CONCLUSION: The F1534C Vssc mutation is common in A. aegypti populations and confers 7-to 16-fold resistance to pyrethroids, DDT, and DCJW in Aedes aegypti. These resistance levels are considerably less than previously reported for the S989P+V1016G mutations. Our results provide useful information for resistance management, specifically the levels of resistance conferred by the most common Vssc mutation in A. aegypti.

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“…15 The process of the occurrence of vector resistance to certain insecticides is influenced by multiple factors, namely genetic (presence of specific gene frequencies), operational (insecticide type and application) and biological (size and characteristics of vector populations). 16 The different of susceptibility status in these two regions due to operational factors, namely vector control through fogging from the program until now is still being used. Fogging is carried out when there is a DHF case report.…”

Section: Results Of Molecular Assays and Sequencing Analysismentioning

confidence: 99%

COMBINED TARGET SITE VGSC MUTATIONS PLAY A PRIMARY ROLE IN PYRETHROID RESISTANT PHENOTYPES OF Aedes aegypti AS DENGUE VECTOR FROM PALU CITY, CENTRAL SULAWESI

Umniyati

2019

IJTID

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It has been reported that Aedes aegypti mosquitoes in Palu City had been resistant to cypermethrin insecticide but the resistance mechanism is not well known. This study aimed to determine the resistance status of Ae. aegypti to cypermethrin and whether the mutation of voltage-gated sodium channel (VGSC) was associated with pyretroid resistance in high and low dengue endemic areas in Palu City. Aedes aegypti collected from each village was reared to adult and assayed for susceptibility test to cypermethrin using the CDC bottle bioassay method. PCR primers of AaSCF1 and AaSCR4 were used for screening of IIS6 VGSC gene mutation. PCR primers of AaSCF7 and AaSCR7 were used for screening of IIIS6 VGSC gene mutation. For an identification of mutation sites were sequenced and aligned to Gene bank (access No. AB914689 and AB914690) for IIS6 VGSC and Gene bank (access No. AB914687 and AB914688) for IIIS6 VGSC gene mutation. The susceptibility status of Ae. aegypti to cypermethrin was resistant in high dengue endemic areas and moderately resistant in low dengue endemic areas. It was found double point mutation at S989P and V1016G in Ae. aegypti from high and low dengue endemic areas in Palu City and there was a single point mutation only in high dengue endemic area at target site V1016G. Aedes aegypti from both high and low dengue endemic areas were resistant to cyperpethrinn and the two alleles had a major role in the occurrence of cypermethrin resistance in Palu City.

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Insecticide resistance is mediated by multiple mechanisms in recently introduced Aedes aegypti from Madeira Island (Portugal)

Cited by 64 publications

References 44 publications

First evidence of resistance to pyrethroid insecticides in ItalianAedes albopictuspopulations 26 years after invasion

First evidence of resistance to pyrethroid insecticides in ItalianAedes albopictuspopulations 26 years after invasion

The F1534C voltage‐sensitive sodium channel mutation confers 7‐ to 16‐fold resistance to pyrethroid insecticides in Aedes aegypti

COMBINED TARGET SITE VGSC MUTATIONS PLAY A PRIMARY ROLE IN PYRETHROID RESISTANT PHENOTYPES OF Aedes aegypti AS DENGUE VECTOR FROM PALU CITY, CENTRAL SULAWESI

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