Insecticide sensitivity of native chloride and sodium channels in a mosquito cell line

Jenson, Lacey J.; Anderson, Troy D.; Bloomquist, Jeffrey R.

doi:10.1016/j.pestbp.2015.11.012

Cited by 8 publications

(4 citation statements)

References 27 publications

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“… aegypti . ANK , ankyrin-2 [33,35]; aPKC , atypical Protein kinase C [74]; β-SPEC , beta-spectrin [35,74,76]; CAa1 , calcium channel alpha subunit [30,31]; CHL , CHARLATAN [25,26] ; NEDD4 , E3 ubiquitin-protein ligase [37,38]; NVY , NERVY [42–44], PUM , pumilio [27], SYT1 , SYNAPTOTAGMIN-1 [87,90]; VGSC , voltage-gated sodium channel [74]. Dotted line indicates indirect interaction due to shared roles in synaptic function.…”

Section: Discussionmentioning

confidence: 99%

Vgsc-interacting proteins are genetically associated with pyrethroid resistance in Aedes aegypti

et al. 2019

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Association mapping of factors that condition pyrethroid resistance in Aedes aegypti has consistently identified genes in multiple functional groups. Toward better understanding of the mechanisms involved, we examined high throughput sequencing data (HTS) from two Aedes aegypti aegypti collections from Merida, Yucatan, Mexico treated with either permethrin or deltamethrin. Exome capture enrichment for coding regions and the AaegL5 annotation were used to identify genes statistically associated with resistance. The frequencies of single nucleotide polymorphisms (SNPs) were compared between resistant and susceptible mosquito pools using a contingency χ2 analysis. The -log10(χ2 p value) was calculated at each SNP site, with a weighted average determined from all sites in each gene. Genes with -log10(χ2 p value) ≥ 4.0 and present among all 3 treatment groups were subjected to gene set enrichment analysis (GSEA). We found that several functional groups were enriched compared to all coding genes. These categories were transport, signal transduction and metabolism, in order from highest to lowest statistical significance. Strikingly, 21 genes with demonstrated association to synaptic function were identified. In the high association group (n = 1,053 genes), several genes were identified that also genetically or physically interact with the voltage-gated sodium channel (VGSC). These genes were eg., CHARLATAN (CHL), a transcriptional regulator, several ankyrin-domain proteins, PUMILIO (PUM), a translational repressor, and NEDD4 (E3 ubiquitin-protein ligase). There were 13 genes that ranked among the top 10%: these included VGSC; CINGULIN, a predicted neuronal gap junction protein, and the aedine ortholog of NERVY (NVY), a transcriptional regulator. Silencing of CHL and NVY followed by standard permethrin bottle bioassays validated their association with permethrin resistance. Importantly, VGSC levels were also reduced about 50% in chl- or nvy-dsRNA treated mosquitoes. These results are consistent with the contribution of a variety of neuronal pathways to pyrethroid resistance in Ae. aegypti.

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Section: Discussionmentioning

confidence: 99%

Vgsc-interacting proteins are genetically associated with pyrethroid resistance in Aedes aegypti

et al. 2019

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“…In contrast, lindane increased chloride current amplitude. This finding is not only the first report of type 2 pyrethroid inhibition of insect voltage-sensitive chloride channels, but also shows that Sua1B cells express native insect ion channels with potential utility for insecticide screening [ 119 ]. The RML12 cell line derived from Aedes albopictus displays spontaneous electrophysiological activity differentiated with 20-HE.…”

Section: Application Of Insect Cell Lines In Pest Managementmentioning

confidence: 77%

Insect Cell-Based Models: Cell Line Establishment and Application in Insecticide Screening and Toxicology Research

Huang

et al. 2023

Insects

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During the past decades, research on insect cell culture has grown tremendously. Thousands of lines have been established from different species of insect orders, originating from several tissue sources. These cell lines have often been employed in insect science research. In particular, they have played important roles in pest management, where they have been used as tools to evaluate the activity and explore the toxic mechanisms of insecticide candidate compounds. This review intends to first briefly summarize the progression of insect cell line establishment. Then, several recent studies based on insect cell lines coupled with advanced technologies are introduced. These investigations revealed that insect cell lines can be exploited as novel models with unique advantages such as increased efficiency and reduced cost compared with traditional insecticide research. Most notably, the insect cell line-based models provide a global and in-depth perspective to study the toxicology mechanisms of insecticides. However, challenges and limitations still exist, especially in the connection between in vitro activity and in vivo effectiveness. Despite all this, recent advances have suggested that insect cell line-based models promote the progress and sensible application of insecticides, which benefits pest management.

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“…There have been a number of studies in which cultured insect cells have been treated with the molting hormone 20‐hydroxy ecdysone (20‐OH E) and which have resulted in a more spindly shape as the cells differentiate into neuron‐like structures with longer processes. In some cases, it appears that voltage‐gated sodium, calcium, potassium or chloride channels have been induced concomitant with the morphological change 37–41 . More recently, it has been shown that 20‐OH E treatment of Aedes RML12 cells led to spontaneous neuroactivity as monitored by multiwell microelectrode array, and that this could be enhanced by treatment with the GABA antagonist gabazine and especially nicotine 42 …”

Section: Lab‐on‐a‐chip or Organ‐on‐a‐chip Technologies For Insecticide Screening Or Mode Of Action Studiesmentioning

confidence: 99%

Pharmaceutical technologies with potential application to insecticide discovery^†

Wing¹

2020

Pest Management Science

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Novel neuroactive insecticides are discovered/registered differently, have a lower value in use, and exert their physiological actions in manners distinct from neuroactive pharmaceuticals, but there are clear similarities in their biochemical modes of action. Insecticides are generally discovered using whole pest insect screens, and this eases difficulties in ‘translational science’ from laboratory to field, as opposed to pharmaceutical translation from biochemical or cell‐based targets to animal models to human clinical trials to registered drug. This paper examines recent trends in pharmaceutical science and identifies some technologies which may represent complementary approaches to insecticide discovery screening and mode of action determination beyond the sound processes in common practice today. Examples will be drawn from nanoparticle delivery of neuroactives, unique ligand–polymer conjugates, proposed advances in insect cell culture following from pharmaceutical cell biology, and laboratory or organ‐on‐a‐chip approaches. It is hoped that these concepts will stimulate novel thinking which may enable discovery of efficacious new neuroactive insecticides. © 2020 Society of Chemical Industry

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Insecticide sensitivity of native chloride and sodium channels in a mosquito cell line

Abstract: 2016-11-02T18:49:00

Cited by 8 publications

References 27 publications

Vgsc-interacting proteins are genetically associated with pyrethroid resistance in Aedes aegypti

Vgsc-interacting proteins are genetically associated with pyrethroid resistance in Aedes aegypti

Insect Cell-Based Models: Cell Line Establishment and Application in Insecticide Screening and Toxicology Research

Pharmaceutical technologies with potential application to insecticide discovery^†

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Insecticide sensitivity of native chloride and sodium channels in a mosquito cell line

Abstract: 2016-11-02T18:49:00

Cited by 8 publications

References 27 publications

Vgsc-interacting proteins are genetically associated with pyrethroid resistance in Aedes aegypti

Vgsc-interacting proteins are genetically associated with pyrethroid resistance in Aedes aegypti

Insect Cell-Based Models: Cell Line Establishment and Application in Insecticide Screening and Toxicology Research

Pharmaceutical technologies with potential application to insecticide discovery†

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About

Pharmaceutical technologies with potential application to insecticide discovery^†