Insemination of mares with low numbers of either unsexed or sexed spermatozoa

Buchanan, B. R.; Seidel, G. E.; McCue, Patrick M.; Schenk, J.L.; Herickhoff, Lisa A.; Squires, E.L.

doi:10.1016/s0093-691x(00)00276-4

Cited by 111 publications

(94 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Buchanan et al (2000) demonstrated that pregnancies could be obtained from insemination of sperm that had been separated into X-and Y-chromosome-bearing populations using a flow cytometer. However, to obtain a full insemination dose (500 million PMS), several days of sorting would be required.…”

Section: Low-dose Insemination-fresh Frozen Sex-sorted Spermmentioning

confidence: 99%

Integration of future biotechnologies into the equine industry

Squires¹

2005

Animal Reproduction Science

Self Cite

View full text Add to dashboard Cite

There has and will continue to be reproductive techniques available that have a positive impact upon the equine breeding industry. This review focuses on semen technologies that have been developed or are in the process of being developed. The use of fluorescent dyes and flow cytometry has provided the researcher and clinician with powerful tools to evaluate several sperm attributes. These procedures have been utilized to evaluate sperm viability, acrosome status, mitochondrial status, DNA integrity and stages of capacitation. Flow cytometry allows several sperm attributes to be evaluated on thousands of spermatozoa in a matter of seconds. Development of procedures for insemination of mares with relatively small numbers of spermatozoa has the potential to change how stallions and their semen are managed. This review discusses the use of insemination of fresh, frozen and sex-sorted spermatozoa in relatively small numbers compared with conventional insemination technologies. The recent acceptance of frozen-thawed semen by many of the major breed registries has stimulated an increase in research on frozen semen. Many of the studies have focused on identifying damage during the freezing and thawing process. Numerous studies also have been conducted to modify freezing extenders so that the sperm are protected during the freezing and thawing process. The production of in vitro-produced embryos is extremely limited in the horse due to the failure of in vitro fertilization. However, intracytoplasmic sperm injection (ICSI) has been used for the production of foals from stallions that have less than typical sperm numbers or from stallions that have died and a limited quantity of frozen semen is available. This technique has been used by several laboratories to produce embryos in vitro. The breeder and veterinarian now have access to techniques that allow assessment of semen quality, improvement of procedures for freezing and thawing and insemination of mares with fewer numbers of spermatozoa. It is likely that the next decade will also produce tremendous advances in semen technologies that can be utilized in the horse industry.

show abstract

Section: Low-dose Insemination-fresh Frozen Sex-sorted Spermmentioning

confidence: 99%

Integration of future biotechnologies into the equine industry

Squires¹

2005

Animal Reproduction Science

Self Cite

View full text Add to dashboard Cite

show abstract

“…Normal AI in mares requires 0.5 to 1 billion spermatozoa. Buchanan et al (2000) reported the birth of the first foal after non-surgical AI with sex-sorted spermatozoa. Two methods helped to reduce the insemination dose.…”

Section: Sex-sorted Equine Spermatozoamentioning

confidence: 99%

“…Two methods helped to reduce the insemination dose. A non-surgical method was described by Buchanan et al (2000), who introduced a flexible AI catheter deep into the uterine horn. More effective was the hysteroscopic insemination, where spermatozoa are deposited directly onto the uterine papilla, the entrance of the utero-tubal junction (Rigby et al 2001).…”

Section: Sex-sorted Equine Spermatozoamentioning

confidence: 99%

Sex selection of sperm in farm animals: status report and developmental prospects

Rath¹,

Barcikowski²,

Graaf³

et al. 2013

Reproduction

View full text Add to dashboard Cite

Pre-selection of spermatozoa based on the relative DNA difference between X-and Y-chromosome bearing populations by flow cytometry is an established method that has been introduced into commercial cattle production. Although several important improvements have increased the sort efficiency, the fertilising ability of sexed spermatozoa based on offspring per insemination is still behind farmers' expectations. The main stress factors, especially on mitochondria, that reduce the lifespan of spermatozoa are described, and new technical as well as biological solutions to maintain the natural sperm integrity and to increase the sorting efficiency are discussed. Among these methods are the identification of Y-chromosome bearing spermatozoa by bi-functionalised gold nanoparticles and triplex hybridisation in vivo as well as new laser-controlled deflection system that replaces the deflection of spermatozoa in the electrostatic field. Additionally, as well as a new nonsurgical transfer system of spermatozoa into the oviduct of cows has been developed and allows a significant reduction of spermatozoa per transfer. Altogether, the improvements made in the recent years will allow a broader use of sex-sorted spermatozoa even in those species that require more cells than cows and sheep.Reproduction (2013) 145 R15-R30

show abstract

“…Since then, DNA content measurements have been used to identify the sex-chromosome bearing sperm populations with good accuracy in semen from at least 23 mammalian species (Garner, 2006;Garner et al, 1983;Lu et al, 2010;Pinkel et al, 1982), and offspring have been produced from sexed sperm of at least seven species, including rabbits (Johnson et al, 1989), humans (Levinson et al, 1995), cattle (Cran et al, 1993), horses (Buchanan et al, 2000), sheep (Catt et al, 1996), dogs (Meyers et al, 2008), cats , elk (Schenk & DeGrofft, 2003), buffalo (Presicce et al, 2005) and dolphins (O'Brien & Robeck, 2006). The first offspring born with flow cytometrically sex sorted spermatozoa was in rabbits after surgical AI into the oviduct (Johnson et al, 1989).…”

Section: Use Of Sexed Semen For Aimentioning

confidence: 99%

Artificial Insemination in Veterinary Science

Heise¹

2012

A Bird's-Eye View of Veterinary Medicine

View full text Add to dashboard Cite

Insemination of mares with low numbers of either unsexed or sexed spermatozoa

Cited by 111 publications

References 23 publications

Integration of future biotechnologies into the equine industry

Integration of future biotechnologies into the equine industry

Sex selection of sperm in farm animals: status report and developmental prospects

Artificial Insemination in Veterinary Science

Contact Info

Product

Resources

About