2023
DOI: 10.3390/ijms242015159
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Insensitive Effects of Inflammatory Cytokines on the Reference Genes of Synovial Fluid Resident-Mesenchymal Stem Cells Derived from Rheumatoid Arthritis Patients

Eun-Yeong Bok,
Saet-Byul Kim,
Gitika Thakur
et al.

Abstract: Mesenchymal stem cells derived from rheumatoid arthritis patients (RA-MSCs) provide an understanding of a variety of cellular and immunological responses within the inflammatory milieu. Sustained exposure of MSCs to inflammatory cytokines is likely to exert an influence on genetic variations, including reference genes (RGs). The sensitive effect of cytokines on the reference genes of RA-SF-MSCs may be a variation factor affecting patient-derived MSCs as well as the accuracy and reliability of data. Here, we co… Show more

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“…In this report, to facilitate future molecular analyses of MSCs cultivated under SFM/XFM, a stability analysis on putative housekeeping genes (HKGs) was performed on adipose-derived MSCs (ASCs) and compared with cells grown in standard FBS-containing conditions. This issue is of particular relevance since different media may impact not only the overall molecular signature of MSCs but also the stability of HKGs [31][32][33][34], thus potentially overestimating or underestimating genes and pathways of interest for a specific clinical application. Under these premises, EF1A and GAPDH inverted their positions in the stability rankings based on the analyzed media (Table 2).…”
Section: Discussionmentioning
confidence: 99%
“…In this report, to facilitate future molecular analyses of MSCs cultivated under SFM/XFM, a stability analysis on putative housekeeping genes (HKGs) was performed on adipose-derived MSCs (ASCs) and compared with cells grown in standard FBS-containing conditions. This issue is of particular relevance since different media may impact not only the overall molecular signature of MSCs but also the stability of HKGs [31][32][33][34], thus potentially overestimating or underestimating genes and pathways of interest for a specific clinical application. Under these premises, EF1A and GAPDH inverted their positions in the stability rankings based on the analyzed media (Table 2).…”
Section: Discussionmentioning
confidence: 99%