Insertion/deletion polymorphism in the promoter of NFKB1 as a potential molecular marker for the risk of recurrence in superficial bladder cancer

Riemann, Kathrin; Becker, Laren; Struwe, Henrike; Rübben, H.; Eisenhardt, Andreas; Siffert, Winfried

doi:10.5414/cpp45423

Cited by 51 publications

(39 citation statements)

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“…12,21 The real-time PCR reaction was performed as described using the Quantitect SYBR Green Kit (Qiagen). 21,22 A complementary DNA dilution series confirmed a PCR efficiency greater than 95%, which was comparable to the efficiency of hβ-actin. Relative NF-κB1 mRNA expression was measured by two-step real-time PCR with hβ-actin as internal control and calculated as 2…”

Section: Thromboelastometrymentioning

confidence: 78%

The NFKB1 Promoter Polymorphism (−94ins/delATTG) Alters Nuclear Translocation of NF-κB1 in Monocytes after Lipopolysaccharide Stimulation and Is Associated with Increased Mortality in Sepsis

Adamzik

Schäfer²,

Frey

et al. 2013

Anesthesiology

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Background: Because the nuclear factor-κB (NF-κB) coupled pathway is believed to amplify inflammation prevailing in sepsis, the authors tested the hypotheses that the insertiondeletion polymorphism (−94ins/delATTG) (1) alters nuclear translocation of nuclear factor-κB and activator protein-1 (NF-κB1) in monocytes after lipopolysaccharide stimulation; (2) affects lipopolysaccharide-induced NF-κB1 messenger RNA expression, tumor necrosis factor α concentrations, and tissue factor activity; and (3) may be associated with increased 30-day mortality in patients with sepsis. Methods: Nuclear translocation of NF-κB1 in monocytes after lipopolysaccharide stimulation from healthy blood donors was performed with immunofluorescence staining (n = 5 each). Lipopolysaccharide-induced NF-κB1 messenger RNA expression was measured with real-time polymerase chain reaction (PCR; n = 60), tumor necrosis factor α concentrations with a multiplexing system kit (n = 60), and tissue factor activity with thromboelastometry (n = 105). In a prospective study, multivariate proportional hazard analysis tested 30-day mortality in patients with sepsis (n = 143). Methods and Results:The homozygous deletion genotype compared with the homozygous insertion genotype was associated with a nearly twofold increase in nuclear translocation of NF-κB1 (P = 0.001), a threefold difference in NF-κB1 What We Already Know about This Topic• The nuclear factor-κB and activator protein-1-coupled signaling pathway of the innate immune system is known to amplify and perpetuate inflammatory and coagulatory mechanisms in sepsis• Genetic variations that alter nuclear factor-κB gene expression could affect pathophysiologic mechanisms and thereby influence mortality in severe sepsis What This Article Tells Us That Is New• A functional insertion-deletion polymorphism in the promoter of nuclear factor-κB and activator protein-1 affected expression and regulation of mediators of inflammation and coagulation in vitro • Expression of the same polymorphism was associated with increased 30-day mortality in patients with severe sepsis Downloaded from anesthesiology.pubs.asahq.org by guest on 06/07/2019

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Section: Thromboelastometrymentioning

confidence: 78%

The NFKB1 Promoter Polymorphism (−94ins/delATTG) Alters Nuclear Translocation of NF-κB1 in Monocytes after Lipopolysaccharide Stimulation and Is Associated with Increased Mortality in Sepsis

Adamzik

Schäfer²,

Frey

et al. 2013

Anesthesiology

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“…Recent studies in humans have revealed that polymorphisms of the NFKB1 gene are associated with oral squamous cell carcinoma (Lin et al, 2006), nasopharyngeal carcinoma (Zhou et al, 2009), colorectal carcinoma (Lewander et al, 2007), and multiple myeloma (Berenson et al, 2001). In bladder cancer, Riemann et al (2007) reported that NFKB1 polymorphism is not associated with an increased susceptibility to transitional cell carcinomas of the bladder in Caucasians, but it is a possible new prognostic marker for recurrence in superficial bladder cancer.…”

Section: Discussionmentioning

confidence: 98%

“…The acknowledged oncogenic potential of NF-kB has led to recent investigations on the relationship between this polymorphism and carcinogenic processes in renal cell carcinoma, colorectal carcinoma, and chronic lymphocytic leukemia, but no correlation was found with the clinical outcome of these tumors (Riemann et al, 2006). Epidemiological studies have investigated the association between NFKB1 polymorphism and risk of bladder cancer in Caucasians but the result did not suggest the relation between NFKB1 polymorphism and risk of bladder cancer (Riemann et al, 2007). We analyzed this polymorphism in our ongoing hospital-based case-control study of bladder cancer in a southwestern Chinese population.…”

Section: Introductionmentioning

confidence: 96%

Insertion/Deletion Polymorphism in the Promoter Region ofNFKB1Gene Increases Susceptibility for Superficial Bladder Cancer in Chinese

Tang

Cui

Deng

et al. 2010

DNA and Cell Biology

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Recently, a functional insertion/deletion polymorphism (-94 insertion/deletion ATTG) in the promoter of NFKB1 gene, which encodes the p50 subunit of nuclear factor-kappaB protein complex, was identified. The aim of this study was to explore the association between this polymorphism and bladder cancer in a Chinese population. The NFKB1 polymorphism was assessed in 207 patients with superficial transitional cell carcinomas in bladder and in 228 age-, sex-, and smoking-matched healthy volunteers. The polymerase chain reaction assay was used to determine the NFKB1 genotypes. Genomic DNA used for the assay was extracted from peripheral blood lymphocytes. This study found that the frequency of ATTG(2) allele in bladder cancer patients was significantly higher than that in control subjects (65.2% vs. 56.1%, p = 0.006, odds ratio = 1.465), suggesting that the functional NFKB1 promoter polymorphism is associated with increased risk for superficial transitional cell carcinoma of the bladder.

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“…This correlation may be associated with the enhanced expression and activity of p50. It has been reported that the insertion allele is associated with an increased activity of the NFKB1 promoter and enhanced NFKB1 mRNA expression (Karban et al, 2004;Riemann et al, 2007). Indeed, at least five studies have reported that the presence of the insertion allele is associated with increased cancer risk and aggressive cancer behavior.…”

Section: Discussionmentioning

confidence: 99%

Association of the genetic polymorphisms of NFKB1 with susceptibility to ovarian cancer

Lp¹,

Ps²,

Hb³

2015

Genet. Mol. Res.

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ABSTRACT. Nuclear factor-kB (NF-kB), a transcription factor that is activated by various stimuli, is associated with the pathogenesis of several cancers. One functional polymorphism, -94 insertion/deletion ATTG (rs28362491), in the human NFKB1 gene (one member of the NF-kB gene family) is associated with increased risk of various cancers. However, only one study has reported that rs28362491 is significantly associated with ovarian cancer. The aim of this study was to analyze the association between single nucleotide polymorphisms (SNPs) and haplotypes in the NFKB1 gene and the risk of ovarian cancer in a Chinese population. We examined the potential association between ovarian cancer and

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Insertion/deletion polymorphism in the promoter of NFKB1 as a potential molecular marker for the risk of recurrence in superficial bladder cancer

Cited by 51 publications

References 0 publications

The NFKB1 Promoter Polymorphism (−94ins/delATTG) Alters Nuclear Translocation of NF-κB1 in Monocytes after Lipopolysaccharide Stimulation and Is Associated with Increased Mortality in Sepsis

The NFKB1 Promoter Polymorphism (−94ins/delATTG) Alters Nuclear Translocation of NF-κB1 in Monocytes after Lipopolysaccharide Stimulation and Is Associated with Increased Mortality in Sepsis

Insertion/Deletion Polymorphism in the Promoter Region ofNFKB1Gene Increases Susceptibility for Superficial Bladder Cancer in Chinese

Association of the genetic polymorphisms of NFKB1 with susceptibility to ovarian cancer

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