Insertion of circularly permuted cyan fluorescent protein into the ligand-binding domain of inositol 1,4,5-trisphosphate receptor for enhanced FRET upon binding of fluorescent ligand

Jahan, Azmeree; Akter, Tahmina M.; Takemoto, Kiwamu; Oura, Tai; Shitara, Akiko; Semba, Shingo; Nezu, Akihiro; Suto, Satoshi; Naga, Takeharu; Tanimura, Akihiko

doi:10.1016/j.ceca.2022.102668

Cell Calcium

2022

DOI: 10.1016/j.ceca.2022.102668

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Insertion of circularly permuted cyan fluorescent protein into the ligand-binding domain of inositol 1,4,5-trisphosphate receptor for enhanced FRET upon binding of fluorescent ligand

Azmeree Jahan

Tahmina M. Akter

Kiwamu Takemoto

et al.

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2024

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Unravelling molecular dynamics in living cells: Fluorescent protein biosensors for cell biology

Sanchez,

Ramirez,

Hodgson

2024

Journal of Microscopy

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Genetically encoded, fluorescent protein (FP)‐based Förster resonance energy transfer (FRET) biosensors are microscopy imaging tools tailored for the precise monitoring and detection of molecular dynamics within subcellular microenvironments. They are characterised by their ability to provide an outstanding combination of spatial and temporal resolutions in live‐cell microscopy. In this review, we begin by tracing back on the historical development of genetically encoded FP labelling for detection in live cells, which lead us to the development of early biosensors and finally to the engineering of single‐chain FRET‐based biosensors that have become the state‐of‐the‐art today. Ultimately, this review delves into the fundamental principles of FRET and the design strategies underpinning FRET‐based biosensors, discusses their diverse applications and addresses the distinct challenges associated with their implementation. We place particular emphasis on single‐chain FRET biosensors for the Rho family of guanosine triphosphate hydrolases (GTPases), pointing to their historical role in driving our understanding of the molecular dynamics of this important class of signalling proteins and revealing the intricate relationships and regulatory mechanisms that comprise Rho GTPase biology in living cells.

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Unravelling molecular dynamics in living cells: Fluorescent protein biosensors for cell biology

Sanchez,

Ramirez,

Hodgson

2024

Journal of Microscopy

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Insertion of circularly permuted cyan fluorescent protein into the ligand-binding domain of inositol 1,4,5-trisphosphate receptor for enhanced FRET upon binding of fluorescent ligand

Cited by 1 publication

References 27 publications

Unravelling molecular dynamics in living cells: Fluorescent protein biosensors for cell biology

Unravelling molecular dynamics in living cells: Fluorescent protein biosensors for cell biology

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