Insertion of cyanobacterial desA gene coding for Δ12-acyl-lipid desaturase increases potato plant resistance to oxidative stress induced by hypothermia

Demin, I. N.; Deryabin, A. N.; Sin’kevich, M. S.; Trunova, T. I.

doi:10.1134/s1021443708050075

Cited by 22 publications

(4 citation statements)

References 21 publications

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“…Transgenic potato plants ( Solanum tuberosum L.) of the early-maturing cultivar Skoroplodnyi from the “Bank of virus-free potato cultivars” of the Russian Potato Research Center, Kraskovo, Russia, were obtained by agrobacterial transformation [ 47 , 48 , 49 ]. Cells of Agrobacterium tumefaciens AGL0 strain comprised the genetic constructs based on expression vector pBI.…”

Section: Methodsmentioning

confidence: 99%

“…Lines were selected that demonstrated the restoration of turgor on the next day after returning to normal cultivation conditions (data not shown). Transgenic desC-licBM 3 lines 87, 112 and 117; transgenic desA-licBM3 lines 69, 73 and 75; and a transgenic licBM3 line, which is a transgenic control, were selected for further analysis [ 47 , 48 , 49 ] ( Supplementary Materials ). Plants of initial cultivar Skoroplodnyi were used as a non-transgenic control.…”

Section: Methodsmentioning

confidence: 99%

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Response of Transgenic Potato Plants Expressing Heterologous Genes of ∆9- or ∆12-Acyl-lipid Desaturases to Phytophthora infestans Infection

Tsypurskaya

Николаева

Lapshin

et al. 2022

Plants

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Late blight is one of the most economically important diseases affecting potato and causing a significant loss in yield. The development of transgenic potato plants with enhanced resistance to infection by Phytophthora infestans may represent a possible approach to solving this issue. A comparative study of the leaf response in control potato plants (S.tuberosum L. cultivar Skoroplodnyi), control transgenic plants expressing the reporter gene of thermostable lichenase (transgenic licBM3 line) and transgenic plants expressing cyanobacterial hybrid genes ∆9-acyl-lipid desaturase (transgenic desC lines) and ∆12-acyl-lipid desaturase (transgenic desA lines) to infection with P. infestans has been performed. The expression of desaturase genes in potato plants enhanced their tolerance to potato late blight agents as compared with the control. The lipid peroxidation level raised in the leaves of the control and transgenic desA plants on third day after inoculation with P. infestans zoospores and remained the same in the transgenic desC plants. The number of total phenolic compounds was increased as early as on the second day after infection in all studied variants and continued to remain the same, except for transgenic desC plants. Accumulation of flavonoids, the main components of the potato leaf phenolic complex, raised on the second day in all studied variants, remained unchanged on the third day in the control plants and decreased in most transgenic plants expressing desaturase genes. The results obtained in our study demonstrate that the expression of genes of Δ9- and Δ12-acyl-lipid desaturases in potato plants enhanced their resistance to P. infestans as compared with the control non-transgenic plants due to concomitant accumulation of phenolic compounds, including flavonoids, in the leaves. All these changes were more pronounced in transgenic desC plants, which indicates that the Δ9-acyllipid desaturase gene appears to be a potential inducer of the production of biological antioxidants in plant cells.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Response of Transgenic Potato Plants Expressing Heterologous Genes of ∆9- or ∆12-Acyl-lipid Desaturases to Phytophthora infestans Infection

Tsypurskaya

Николаева

Lapshin

et al. 2022

Plants

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“…ROS interact nonspecifically with many cellular components, trig gering peroxidative reactions and causing significant damage to essential macromolecules, such as photo synthetic pigments, proteins, nucleic acids, and lipids, and especially damaging the membranes [2]. Cold stress increases membrane permeability characterized by the electrolyte leakage from tissues [3]. Cell mem branes also are damaged by ROS, resulting in mem brane lipid peroxidation that can be used as a bio chemical marker of cold stress in plants [4].…”

Section: Introductionmentioning

confidence: 99%

Change in antioxidant responses against oxidative damage in black chickpea following cold acclimation

Nazari

Amiri

Mehraban

et al. 2012

Russ J Plant Physiol

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Cold stress is an important factor affecting chickpea (Cicer arietinum L.) plants in winter and early spring. We evaluated the effects of cold stress by measuring lipid peroxidation, membrane permeability, and some enzyme activities involved in the ROS scavenging system under acclimation and non acclimation con ditions in black chickpea Kaka, a popular genotype planted, and accession 4322, as a landrace genotype. Under non acclimation conditions, the genotype 4322 prevented the H 2 O 2 accumulation more efficiently, which led to a decrease in lipid peroxidation and membrane permeability compared to Kaka. Studying the activities of antioxidant enzymes showed that catalase was more effective enzyme in cell protection against H 2 O 2 in 4322 plants. Such response in acclimated plants was more pronounced than in control and non acclimated plants. In this study, the increase in guaiacol peroxidase and ascorbate peroxidase activities did not preserve cell membranes from oxidative damage in Kaka plants. It was observed that short term acclima tion can induce greater cold tolerance upon the increase of oxidative stress in chickpea plants. This was due to low levels of MDA and electrolyte leakage index, indicating the lower lipid peroxidation and higher mem brane stability under the cold stress compared to non acclimated plants.

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“…It has been reported that TiO 2 NPs induce certain stress-combative mechanisms, such as an improvement of the defense mechanism against ROS accumulation in plant intercellular space, which has been shown to attenuate H 2 O 2 induction [60]. An increase in MDA content has been described as the initial stage of plant injury, which shows the rate of membrane lipid peroxidation [61]. This reveals the extent to which plants face this serious problem.…”

Section: Discussionmentioning

confidence: 99%

Co-Application of 24-Epibrassinolide and Titanium Oxide Nanoparticles Promotes Pleioblastus pygmaeus Plant Tolerance to Cu and Cd Toxicity by Increasing Antioxidant Activity and Photosynthetic Capacity and Reducing Heavy Metal Accumulation and Translocation

et al. 2022

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The integrated application of nanoparticles and phytohormones was explored in this study as a potentially eco-friendly remediation strategy to mitigate heavy metal toxicity in a bamboo species (Pleioblastus pygmaeus) by utilizing titanium oxide nanoparticles (TiO2-NPs) and 24-epibrassinolide (EBL). Hence, an in vitro experiment was performed to evaluate the role of 100 µM TiO2 NPs and 10−8 M 24-epibrassinolide individually and in combination under 100 µM Cu and Cd in a completely randomized design using four replicates. Whereas 100 µM of Cu and Cd reduced antioxidant activity, photosynthetic capacity, plant tolerance, and ultimately plant growth, the co-application of 100 µM TiO2 NPs and 10−8 M EBL+ heavy metals (Cu and Cd) resulted in a significant increase in plant antioxidant activity (85%), nonenzymatic antioxidant activities (47%), photosynthetic pigments (43%), fluorescence parameters (68%), plant growth (39%), and plant tolerance (41%) and a significant reduction in the contents of malondialdehyde (45%), hydrogen peroxide (36%), superoxide radical (62%), and soluble protein (28%), as well as the percentage of electrolyte leakage (49%), relative to the control. Moreover, heavy metal accumulation and translocation were reduced by TiO2 NPs and EBL individually and in combination, which could improve bamboo plant tolerance.

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Insertion of cyanobacterial desA gene coding for Δ12-acyl-lipid desaturase increases potato plant resistance to oxidative stress induced by hypothermia

Cited by 22 publications

References 21 publications

Response of Transgenic Potato Plants Expressing Heterologous Genes of ∆9- or ∆12-Acyl-lipid Desaturases to Phytophthora infestans Infection

Response of Transgenic Potato Plants Expressing Heterologous Genes of ∆9- or ∆12-Acyl-lipid Desaturases to Phytophthora infestans Infection

Change in antioxidant responses against oxidative damage in black chickpea following cold acclimation

Co-Application of 24-Epibrassinolide and Titanium Oxide Nanoparticles Promotes Pleioblastus pygmaeus Plant Tolerance to Cu and Cd Toxicity by Increasing Antioxidant Activity and Photosynthetic Capacity and Reducing Heavy Metal Accumulation and Translocation

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