Insertional Mutagenesis To Generate Lantibiotic Resistance in
            <i>Lactococcus lactis</i>

Guinane, Caitriona M.; Cotter, Paul D.; Lawton, Elaine M.; Hill, Colin; Ross, R. Paul

doi:10.1128/aem.02351-06

Cited by 3 publications

(1 citation statement)

References 31 publications

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“…Deletion of the 801-bp fragment of gadD3 in the ⌬gadD3 mutant was confirmed by sequencing. An L. lactis IL1403 gadB insertion mutant was created by cloning 350 bp of the 5Ј end of the gene into plasmid pORI19 as previously described (12).…”

Section: Methodsmentioning

confidence: 99%

Glutamate Decarboxylase-Mediated Nisin Resistance in Listeria monocytogenes

Begley

Cotter

Hill

et al. 2010

Appl Environ Microbiol

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Analysis of a complete set of glutamate decarboxylase (gad) mutants of Listeria monocytogenes strain LO28 (⌬gadD1, ⌬gadDT1, ⌬gadD2, ⌬gadT2, and ⌬gadD3 mutants) revealed that the ⌬gadD1 mutant is impaired in its ability to tolerate exposure to both sublethal and lethal levels of the lantibiotic nisin. gadD1 is strain variable and is found only in approximately 50% of L. monocytogenes strains. Growth and survival experiments revealed that possession of gadD1 correlates with a higher degree of tolerance to nisin. Significantly, a similar finding using a gadB mutant of L. lactis IL1403 implies that this may be a general phenomenon in Grampositive bacteria. Our findings thus suggest that the specific inhibition of GAD activity or a reduction in the levels of free glutamate may prevent the growth of otherwise resistant GAD ؉ bacteria in foods where low pH and/or nisin is used as a preservative.

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Section: Methodsmentioning

confidence: 99%