Inside Back Cover: Transcription of Click‐Linked DNA in Human Cells (Angew. Chem. Int. Ed. 9/2014)

The Risk of the Preparation of Artificial DNAs via an Interrupted Automated Solid-Phase Triester Method

“…The insertion of triazole modifications within the natural strand results in lowering of T m values. According to a recent investigation by Birts et al, [30] the triazole modification (oligonucleotide functionalized with a 5 -azide is ligated to an oligonucleotide with a 3 -alkyne through Cu I ) is an alternative to the current enzymatic approaches for DNA assembly. This example has been described as the first model for a non-natural DNA linker being operative in human cells.…”

“…In fact, this study shows that still the flexibility of DNA is maintained in the presence of a relatively small click-linked DNA sites. From the various triazole internucleotide linkages, the conformation for the interrupted 3 -5 chain, as synthesized by Birts et al, [30] is the most attractive one to guarantee to some extent the backbone flexibility necessary for accommodation with the complementary natural strand. The role of this triazole fragment has been described before by Brik et al [31] as a peptide alternative of HIV-1 protease inhibitors.…”