Inside Cover: Two‐Way Nanopore Sensing of Sequence‐Specific Oligonucleotides and Small‐Molecule Targets in Complex Matrices Using Integrated DNA Supersandwich Structures (Angew. Chem. Int. Ed. 7/2013)

Abstract

DNA supersandwich structures work as gatekeepers for solid‐state nanopores as part of a target‐specific nanofluidic sensor for both oligonucleotides and small molecules, as shown by F. Xia, W. Guo et al. in their Communication on The DNA superstructures enhance the sensitivity and reduce the background signal, so that the biohybrid nanodevice also works well in complex mixtures, such as mammalian serum.

Biomimetic nanochannels based biosensor for ultrasensitive and label-free detection of nucleic acids

“…In a certain concentration range, the modified nanochannel displayed a response to c-DNA. The detection limit was determined according to the method reported in literature (Xia et al, 2013). When nc-DNA oligomer was interacted with the probe DNA-modified nanochannel, the R -/+ change ratio of nc-DNA was found to be about 0.48.…”

“…Recently, the highly specific and sensitive nanochannels based biosensors have been developed as efficient sensing platforms in detecting nucleic acids (Xia et al, 2013;Ali et al, 2014;Farimani et al, 2010). The principle of the nanochannel-based DNA biosensors is that the immobilized probe specifically hybridizes with singlestranded target nucleic acids on the channel surface.…”

“…The hybridization event changes the surface charge density and/or effective diameter of the nanochannel, and triggers the membrane current change, which can be recorded by instrument. For instance, Xia et al (Xia et al, 2013) developed a novel nanochannel-based method for detecting oligonucleotides through forming complex supersandwich structures capable of blocking the nanochannel. And this system could also detect DNA in complex matrices and serum sample.…”

Biomolecule‐Functionalized Solid‐State Ion Nanochannels/Nanopores: Features and Techniques

“…The on‐off ratio before and after ATP treatment approached to 10 5 –10 6 due to the amplification from DNA supersandwich in nanochannels. Likewise, other signal amplification cases about the highly sensitive and specific detections of DNA and ATP were also performed by using DNA supersandwich‐functionalized nanochannels, where the detection limits for one‐mismatch DNA and ATP were 10 × 10 −15 and 1 × 10 −9 m , respectively. The similar signal amplification strategy was also adopted to detect Zn 2+ with high selectivity and sensitivity.…”

Smart Bioinspired Nanochannels and their Applications in Energy‐Conversion Systems

“…[6] Smart bioinspired nano channels with different shapes and compositions have been fabri cated, and these nanochannels can respond to various stimuli, such as pH, [7] light, [8] temperature, [9] specific ions, [10] and mole cules. [11] Compared with their fragile biological counterparts, smart bioinspired nanochannels possess excellent mechanical robustness, controllable geometry, tunable surface properties, and good chemical stability. [12] These advantages make them useful for many potential applications, ranging from molecular filters to biosensors to energy conversion devices.…”