Inside-Out: From Endosomes to Extracellular Vesicles in Fungal RNA Transport

Kwon, Seomun; Tisserant, Constance; Tulinski, Markus; Weiberg, Arne; Feldbrügge, Michael

doi:10.20944/preprints201911.0213.v1

Cited by 5 publications

(7 citation statements)

References 90 publications

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“…EVs are emerging as mediators of plant-pathogen communication, particularly as vehicles for transfer of RNA (reviewed in [ 81 ]). On the plant side, studies to date have mostly focused on the role of sRNAs [ 19 , 20 ] and proteins [ 22 , 23 , 82 ] in EVs, while only protein cargos have been examined in EVs of phytopathogenic fungi [ 9 , 24 ].…”

Section: Discussionmentioning

confidence: 99%

mRNA Inventory of Extracellular Vesicles from Ustilago maydis

Kwon

Rupp

Brachmann

et al. 2021

JoF

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Extracellular vesicles (EVs) can transfer diverse RNA cargo for intercellular communication. EV-associated RNAs have been found in diverse fungi and were proposed to be relevant for pathogenesis in animal hosts. In plant-pathogen interactions, small RNAs are exchanged in a cross-kingdom RNAi warfare and EVs were considered to be a delivery mechanism. To extend the search for EV-associated molecules involved in plant-pathogen communication, we have characterised the repertoire of EV-associated mRNAs secreted by the maize smut pathogen, Ustilago maydis. For this initial survey, we examined EV-enriched fractions from axenic filamentous cultures that mimic infectious hyphae. EV-associated RNAs were resistant to degradation by RNases and the presence of intact mRNAs was evident. The set of mRNAs enriched inside EVs relative to the fungal cells are functionally distinct from those that are depleted from EVs. mRNAs encoding metabolic enzymes are particularly enriched. Intriguingly, mRNAs of some known effectors and other proteins linked to virulence were also found in EVs. Furthermore, several mRNAs enriched in EVs are also upregulated during infection, suggesting that EV-associated mRNAs may participate in plant-pathogen interactions.

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Section: Discussionmentioning

confidence: 99%

mRNA Inventory of Extracellular Vesicles from Ustilago maydis

Kwon

Rupp

Brachmann

et al. 2021

JoF

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“…vasinfectum were described to induce a phytotoxic response in plants (Bleackley et al, 2019b ), which reinforce this hypothesis. This subject has been discussed in detail in recent reviews (Huang et al, 2019 ; Kwon et al, 2020 ).…”

Section: Cell-to-cell Communication Mediated By Evsmentioning

confidence: 99%

Extracellular Vesicles in Fungi: Past, Present, and Future Perspectives

Rizzo

Rodrigues

Janbon

2020

Front. Cell. Infect. Microbiol.

109

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Extracellular vesicles (EVs) have garnered much interest in the cell biology and biomedical research fields. Many studies have reported the existence of EVs in all types of living cells, including in fifteen different fungal genera. EVs play diverse biological roles, from the regulation of physiological events and response to specific environmental conditions to the mediation of highly complex interkingdom communications. This review will provide a historical perspective on EVs produced by fungi and an overview of the recent discoveries in the field. We will also review the current knowledge about EV biogenesis and cargo, their role in cell-to-cell interactions, and methods of EV analysis. Finally, we will discuss the perspectives of EVs as vehicles for the delivery of biologically active molecules.

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“…EVs are emerging as mediators of plant-pathogen communication, particularly as vehicles for transfer of RNA (Reviewed in [77]). On the plant side, studies to date have mostly focused on the role of sRNAs [19,20] and proteins [22,23,78] in EVs, while only protein cargos have been examined in EVs of phytopathogenic fungi [9,24].…”

Section: Discussionmentioning

confidence: 99%

mRNA inventory of extracellular vesicles from Ustilago maydis

Kwon¹,

Rupp²,

Brachmann³

et al. 2021

Preprint

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Extracellular vesicles (EVs) can transfer diverse RNA cargo for intercellular signalling. EV-associated RNAs have been found in diverse fungi and were proposed to be relevant for pathogenesis in animal hosts. In plant-pathogen interactions, small RNAs are exchanged in a cross-kingdom RNAi warfare and EVs were considered to be a delivery mechanism. To extend the search for EV-associated molecules involved in plants-pathogen communication, we have characterised the repertoire of EV-associated mRNAs secreted by the maize smut pathogen, Ustilago maydis. For this initial survey, EVs were isolated from axenic filamentous cultures that mimic infectious hyphae. The EV-associated RNAs were resistant to degradation by RNases and the presence of intact mRNAs was evident. The set of mRNAs enriched inside EVs relative to the fungal cells are functionally distinct from those that are depleted from EVs, particularly overrepresented in metabolic enzyme activities. Intriguingly, mRNAs of some known effectors and other proteins linked to virulence were found in EVs. Furthermore, several mRNAs enriched in EVs are also upregulated during infection, suggesting that EV-associated mRNAs may participate in plant-pathogen interaction.

show abstract

Inside-Out: From Endosomes to Extracellular Vesicles in Fungal RNA Transport

Cited by 5 publications

References 90 publications

mRNA Inventory of Extracellular Vesicles from Ustilago maydis

mRNA Inventory of Extracellular Vesicles from Ustilago maydis

Extracellular Vesicles in Fungi: Past, Present, and Future Perspectives

mRNA inventory of extracellular vesicles from Ustilago maydis

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