Insight in Hypoxia-Mimetic Agents as Potential Tools for Mesenchymal Stem Cell Priming in Regenerative Medicine

Nowak-Stępniowska, Agata; Osuchowska, Paulina Natalia; Fiedorowicz, Henryk; Trafny, Elżbieta Anna

doi:10.1155/2022/8775591

Cited by 27 publications

(18 citation statements)

References 169 publications

(226 reference statements)

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“…Acidosis-primed MSCs could release the specified exosomes, which could increase the anti-inflammatory T cell subtypes in vitro [ 62 ]. Enhanced proliferation and neovascularization capacity were reported in the MSCs primed by hypoxia [ 63 ]. Inflammatory cytokine IL-1 β -primed hUC-MSCs could release of growth factors to promote epidermal substitute engraftment and wound healing in vivo [ 64 ].…”

Section: Discussionmentioning

confidence: 99%

Human Umbilical Cord Mesenchymal Stem Cells Encapsulated with Pluronic F-127 Enhance the Regeneration and Angiogenesis of Thin Endometrium in Rat via Local IL-1β Stimulation

Zhou

Lei

Wang³

et al. 2022

Stem Cells International

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Thin endometrium (< 7 mm) could cause low clinical pregnancy, reduced live birth, increased spontaneous abortion, and decreased birth weight. However, the treatments for thin endometrium have not been well developed. In this study, we aim to determine the role of Pluronic F-127 (PF-127) encapsulation of human umbilical cord mesenchymal stem cells (hUC-MSCs) in the regeneration of thin endometrium and its underlying mechanism. Thin endometrium rat model was created by infusion of 95% ethanol. Thin endometrium modeled rat uterus were treated with saline, hUC-MSCs, PF-127, or hUC-MSCs plus PF-127 separately. Regenerated rat uterus was measured for gene expression levels of angiogenesis factors and histological morphology. Angiogenesis capacity of interleukin-1 beta (IL-1β)-primed hUC-MSCs was monitored via quantitative polymerase chain reaction (q-PCR), Luminex assay, and tube formation assay. Decreased endometrium thickness and gland number and increased inflammatory factor IL-1β were achieved in the thin endometrium rat model. Embedding of hUC-MSCs with PF-127 could prolong the hUC-MSCs retaining, which could further enhance endometrium thickness and gland number in the thin endometrium rat model via increasing angiogenesis capacity. Conditional medium derived from IL-1β-primed hUC-MSCs increased the concentration of angiogenesis factors (basic fibroblast growth factor (bFGF), vascular endothelial growth factors (VEGF), and hepatocyte growth factor (HGF)). Improvement in the thickness, number of glands, and newly generated blood vessels could be achieved by uterus endometrium treatment with PF-127 and hUC-MSCs transplantation. Local IL-1β stimulation-primed hUC-MSCs promoted the release of angiogenesis factors and may play a vital role on thin endometrium regeneration.

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Section: Discussionmentioning

confidence: 99%

Human Umbilical Cord Mesenchymal Stem Cells Encapsulated with Pluronic F-127 Enhance the Regeneration and Angiogenesis of Thin Endometrium in Rat via Local IL-1β Stimulation

Zhou

Lei

Wang³

et al. 2022

Stem Cells International

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“…However, due to access difficulty to such equipment in many laboratories, hypoxia-mimetic agents have been used as alternative models. CoCl2 is one of the most commonly used hypoxia-mimetic chemicals in cell culture (Muñoz-Sánchez and Chánez-Cárdenas, 2019;Nowak-Stępniowska et al, 2022). Upregulation of HIF-1α in B16F10 melanoma cells induced by CoCl2 (Fig.…”

Section: Discussionmentioning

confidence: 99%

“…Based on these results, IDF-11774 concentrations of 2.5 mM and 5.0 mM were chosen for the following experiments. CoCl2 is one of the most commonly used hypoxia-mimic chemicals in cell culture (Muñoz-Sánchez and Chánez-Cárdenas, 2019; Nowak-Stępniowska et al, 2022). Therefore, hypoxic condition was prepared using CoCl2 in the present study.…”

Section: Idf-11774 Reduces Cocl 2 -Induced Hif-1α α Upregulation In C...mentioning

confidence: 99%

Anti-Tumor Effect of IDF-11774, an Inhibitor of Hypoxia-Inducible Factor-1, on Melanoma

Kim

Lee

2022

Biomol Ther (Seoul)

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Melanoma is one of the most aggressive skin cancers. Hypoxia contributes to the aggressiveness of melanoma by promoting cancer growth and metastasis. Upregulation of cyclin D1 can promote uncontrolled cell proliferation in melanoma, whereas stimulation of cytotoxic T cell activity can inhibit it. Epithelial mesenchymal transition (EMT) plays a critical role in melanoma metastasis. Hypoxia-inducible factor-1α (HIF-1α) is a main transcriptional mediator that regulates many genes related to hypoxia. CoCl2 is one of the most commonly used hypoxia-mimetic chemicals in cell culture. In this study, inhibitory effects of IDF-11774, an inhibitor of HIF-1α, on melanoma growth and metastasis were examined using cultured B16F10 mouse melanoma cells and nude mice transplanted with B16F10 melanoma cells in the presence or absence of CoCl2-induced hypoxia. IDF-11774 reduced HIF-1α upregulation and cell survival, but increased cytotoxicity of cultured melanoma cells under CoCl2-induced hypoxia. IDF-11774 also reduced tumor size and local invasion of B16F10 melanoma in nude mice along with HIF-1α downregulation. Expression levels of cyclin D1 in melanoma were increased by CoCl2 but decreased by IDF-11774. Apoptosis of melanoma cells and infiltration of cytotoxic T cells were increased in melanoma after treatment with IDF-11774. EMT was stimulated by CoCl2, but restored by IDF-11774. Overall, IDF-11774 inhibited the growth and metastasis of B16F10 melanoma via HIF-1α downregulation. The growth of B16F10 melanoma was inhibited by cyclin D1 downregulation and cytotoxic T cell stimulation. Metastasis of B16F10 melanoma was inhibited by EMT suppression.

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“…Their important role in tissue regeneration and repair processes, as well as in angiogenesis, was also indicated [ 22 , 23 , 24 ]. In addition, the fact that MSCs have been studied for more than 30 years and are used in more than 1130 clinical trials, is not to be underestimated [ 25 ]. Further, the MSC culture under reduced (physiological) oxygen conditions was used because the literature and our own research show that application in cell culture physiological normoxia results in faster stem cells division, longer maintenance of cellular stemness in culture (maintenance of expression of markers typical of stem cells and mesenchymal stem cells), better cell condition, and slower ageing [ 25 , 26 , 27 , 28 , 29 ].…”

Section: Introductionmentioning

confidence: 99%

“…In addition, the fact that MSCs have been studied for more than 30 years and are used in more than 1130 clinical trials, is not to be underestimated [ 25 ]. Further, the MSC culture under reduced (physiological) oxygen conditions was used because the literature and our own research show that application in cell culture physiological normoxia results in faster stem cells division, longer maintenance of cellular stemness in culture (maintenance of expression of markers typical of stem cells and mesenchymal stem cells), better cell condition, and slower ageing [ 25 , 26 , 27 , 28 , 29 ]. In addition, these conditions are more similar to those of tissues in the human body (about 2–7%) than the 21% (ambient) oxygen concentration used in standard cell cultures.…”

Section: Introductionmentioning

confidence: 99%

Study on βTCP/P(3HB) Scaffolds—Physicochemical Properties and Biological Performance in Low Oxygen Concentration

Skibiński

Czechowska

Cichoń

et al. 2022

IJMS

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The search for new materials for bone regenerative purposes is still ongoing. Therefore, we present a series of newly constructed composites based on β tricalcium phosphate (βTCP) and poly(3-hydroxybutyrate) bacteria-derived biopolymer (P(3HB)) in the form of 3D scaffolds with different pore sizes. To improve the polymer attachment to the βTCP surface, the etching of ceramic sinters, using citric acid, was applied. As expected, pre-treatment led to the increase in surface roughness and the creation of micropores facilitating polymer adhesion. In this way, the durability and compressive strength of the ceramic–polymer scaffolds were enhanced. It was confirmed that P(3HB) degrades to 3-hydroxybutyric acid, which broadens applications of developed materials in bone tissue engineering as this compound can potentially nourish surrounding tissues and reduce osteoporosis. Moreover, to the best of our knowledge, it is one of the first studies where the impact of βTCP/P(3HB) scaffolds on mesenchymal stem cells (MSCs), cultured in lowered (5%) oxygen concentration, was assessed. It was decided to use a 5% oxygen concentration in the culture to mimic the conditions that would be found in damaged bone in a living organism during regeneration. Scaffolds enabled cell migration and sufficient flow of the culture medium, ensuring high cell viability. Furthermore, in composites with etched βTCP, the MSCs adhesion was facilitated by hydrophilic ceramic protrusions which reduced hydrophobicity. The developed materials are potential candidates for bone tissue regeneration. Nevertheless, to confirm this hypothesis, in vivo studies should be performed.

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Insight in Hypoxia-Mimetic Agents as Potential Tools for Mesenchymal Stem Cell Priming in Regenerative Medicine

Cited by 27 publications

References 169 publications

Human Umbilical Cord Mesenchymal Stem Cells Encapsulated with Pluronic F-127 Enhance the Regeneration and Angiogenesis of Thin Endometrium in Rat via Local IL-1β Stimulation

Human Umbilical Cord Mesenchymal Stem Cells Encapsulated with Pluronic F-127 Enhance the Regeneration and Angiogenesis of Thin Endometrium in Rat via Local IL-1β Stimulation

Anti-Tumor Effect of IDF-11774, an Inhibitor of Hypoxia-Inducible Factor-1, on Melanoma

Study on βTCP/P(3HB) Scaffolds—Physicochemical Properties and Biological Performance in Low Oxygen Concentration

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