Insight into anti-diabetic effect of low dose of stevioside

Ilić, Vladimirka; Vukmirović, Saša; Stilinović, Nebojša; Čapo, Ivan; Arsenovic, M.; Milijašević, B.

doi:10.1016/j.biopha.2017.03.045

Cited by 41 publications

(23 citation statements)

References 23 publications

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“…The safety of SVT as a sweetener has been evaluated by the Joint Food and Agricultural Organization and the World Organization Expert Committee on Food Additives. SVT has been used in the food industry since 2011 …”

Section: Introductionmentioning

confidence: 99%

Stevioside inhibits experimental fibrosis by down‐regulating profibrotic Smad pathways and blocking hepatic stellate cell activation

Casas‐Grajales

Alvarez-Suarez

Ramos‐Tovar

et al. 2019

Basic Clin Pharma Tox

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Liver cirrhosis is associated with increased morbidity and mortality with important health and social consequences; however, an effective treatment has not been found yet. Previous reports have shown some beneficial effects of stevioside (SVT) in different diseases, but the ability of SVT to inhibit liver cirrhosis has not been reported. Therefore, we studied the potential of this diterpenoid to inhibit liver cirrhosis induced by thioacetamide, a model that shares many similarities with the human disease, and investigated the possible underlying molecular mechanism using in vivo and in vitro approaches. Cirrhosis was induced in male Wistar rats by chronic thioacetamide administration (200 mg/kg) intraperitoneally three times per week.Rats received saline or SVT (20 mg/kg) two times daily intraperitoneally. In addition, co-cultures were incubated with either lipopolysaccharide or ethanol. Liver fibrosis, hepatic stellate cells activation, metalloproteinases activity, canonical and non-canonical Smads pathway and expression of several profibrogenic genes were evaluated. Thioacetamide activated hepatic stellate cells and distorted the liver parenchyma with the presence of abundant thick bands of collagen. In addition, thioacetamide up-regulated the protein expression of α-smooth muscle actin, transforming growth factor-β1, metalloproteinases-9,-2 and -13 and overstimulate the canonical and non-canonical Smad pathways. SVT administration inhibited all of these changes. In vitro, SVT inhibited the up-regulation of several genes implicated in cirrhosis when cells were exposed to lipopolysaccharides or ethanol. We conclude that SVT inhibited liver damage by blocking hepatic stellate cells activation, down-regulating canonical and non-canonical profibrotic Smad pathways.

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Section: Introductionmentioning

confidence: 99%

Stevioside inhibits experimental fibrosis by down‐regulating profibrotic Smad pathways and blocking hepatic stellate cell activation

Casas‐Grajales

Alvarez-Suarez

Ramos‐Tovar

et al. 2019

Basic Clin Pharma Tox

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“…Although, previous studies have reported the hypoglycemic property of stevioside [14] , more detailed studies are still needed to understand the mechanism by which it improves T2DM and prevents its associated complications. This present study therefore seeks to investigate the effects of stevioside on oxidative damage in a high fat fed/streptozotocin-induced T2DM rat model.…”

Section: Introductionmentioning

confidence: 99%

Stevioside modulates oxidative damage in the liver and kidney of high fat/low streptozocin diabetic rats

Rotimi

Adelani

et al. 2018

Heliyon

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This study investigated the potential of stevioside to prevent oxidative DNA damage in the liver and kidney of type 2 diabetes mellitus (T2DM) using high fat-low streptozocin rat model. Rats were treated daily with 12.5, 25 and 50 mg/kg stevioside orally for 21 days. Levels of biomarkers of T2DM, lipid profile and oxidative stress were assayed spectrophotometrically. The DNA ladder assay method was used to assess DNA fragmentation in the liver and kidney while computational analysis was used to predict the mechanisms of antidiabetic properties of stevioside. Stevioside significantly (p < 0.05) decreased the levels of plasma glucose, insulin, dipeptidyl peptidase IV and activities of kidney angiotensin converting enzyme. Stevioside significantly reduced oxidative stress by decreasing the levels of lipid peroxidation and nitric oxide in the liver and kidney; thereby, reducing the extent of DNA fragmentation in the liver and kidney of the diabetic rats. The in silico analysis showed that the ability of stevioside to exert these effects is linked to its inhibition of beta-adrenergic receptor kinase and G-protein-coupled receptor kinase. The results of this study suggest that the prevention of DNA fragmentation may be an additional benefit of the use of stevioside in the management of T2DM.

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“…While the gut microbial action on steviol glycosides has been extensively studied, the effect of such glycosides on the gut microbiota community ecosystem remains poorly understood. Concentration ranges for the various stevia compounds used in this study were chosen based on the published scientific studies for each of the compounds [8,16,17,47,49] and calculated to reflect concentration ranges within the acceptable daily intake (ADI) established by the Joint FAO/WHO Expert Committee on Food Additives (JECFA) [50]. Using pure samples of the stevia extract, glycosides (stevioside and Reb A), and their aglycon steviol, we showed that the agents have an inhibitory effect on our bacterial models.…”

Section: Discussionmentioning

confidence: 99%

“…The hypoglycemic properties of steviol glycosides [7,8] may enhance insulin release and utilization [7,9,10], while aglycon steviol was shown to induce insulin secretion by directly acting on β-cells [11,12]. However, some studies could not ascertain the action of stevia in glucose regulation, nor could they find evidence that it could rescue mice with glucose intolerance, induced by a high-fat diet.…”

Section: Introductionmentioning

confidence: 99%

Anti-Quorum Sensing Activity of Stevia Extract, Stevioside, Rebaudioside A and Their Aglycon Steviol

Markus

Teralı

et al. 2020

Molecules

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Governments are creating regulations for consumers to reduce their sugar intake, prompting companies to increase the ratio of artificial sweeteners in their products. However, there is evidence of some deleterious effects ascribed to the aforementioned synthetic agents and therefore consumers and food manufacturers have turned their attention to natural dietary sweeteners, such as stevia, to meet their sweetening needs. Stevia is generally considered safe; however, emerging scientific evidence has implicated the agent in gut microbial imbalance. In general, regulation of microbial behavior is known to depend highly on signaling molecules via quorum sensing (QS) pathways. This is also true for the gut microbial community. We, therefore, evaluated the possible role of these stevia-based natural sweeteners on this bacterial communication pathway. The use of a commercial stevia herbal supplement resulted in an inhibitory effect on bacterial communication, with no observable bactericidal effect. Purified stevia extracts, including stevioside, rebaudioside A (Reb A), and steviol revealed a molecular interaction, and possible interruption of Gram-negative bacterial communication, via either the LasR or RhlR receptor. Our in-silico analyses suggest a competitive-type inhibitory role for steviol, while Reb A and stevioside are likely to inhibit LasR-mediated QS in a non-competitive manner. These results suggest the need for further safety studies on the agents.

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Insight into anti-diabetic effect of low dose of stevioside

Cited by 41 publications

References 23 publications

Stevioside inhibits experimental fibrosis by down‐regulating profibrotic Smad pathways and blocking hepatic stellate cell activation

Stevioside inhibits experimental fibrosis by down‐regulating profibrotic Smad pathways and blocking hepatic stellate cell activation

Stevioside modulates oxidative damage in the liver and kidney of high fat/low streptozocin diabetic rats

Anti-Quorum Sensing Activity of Stevia Extract, Stevioside, Rebaudioside A and Their Aglycon Steviol

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