Insight into c-di-GMP Regulation in Anammox Aggregation in Response to Alternating Feed Loadings

Guo, Yongzhao; Liu, Sitong; Tang, Xi; Wang, Chao; Niu, Zhao; Feng, Ying

doi:10.1021/acs.est.6b06396

Cited by 89 publications

(15 citation statements)

References 53 publications

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“…Metabolic products were extracted from the anammox consortia samples in autotrophic and mixotrophic groups. The protocols used to extract metabolic products were conducted according to those described previously (Guo et al, 2017;Tang et al, 2018b). In brief, anammox consortia suspension was collected by centrifugation, washed using Phosphate buffer saline (PBS), and sonicated using a sonicator.…”

Section: Lc-ms-based Metabolomic Profiling and Quantitation Analysismentioning

confidence: 99%

Discrepant gene functional potential and cross-feedings of anammox bacteria Ca. Jettenia caeni and Ca. Brocadia sinica in response to acetate

et al. 2019

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Although the enhancement of anammox performance for wastewater treatment due to the addition of small amount of acetate has been reported, discrepant metabolic responses of different anammox species have not been experimentally evaluated. Based on metagenomics and metatranscriptomic data, we investigated the competitiveness between two typical anammox species, Candidatus Jettenia caeni (J. caeni) and Candidatus Brocadia sinica (B. sinica), in anammox consortia under mixotrophic condition, where complex metabolic interactions among anammox bacteria and heterotrophs also changed with acetate addition. Contrary to J. caeni, the dissimilatory nitrate reduction to ammonium pathway of B. sinica was markedly stimulated for improving nitrogen removal. More acetate metabolic pathways and up-regulated AMP-acs expression for acetyl-CoA synthesis in B. sinica contributed to its superiority in acetate utilization. Interestingly, cross-feedings, including the nitrogen cycle, amino acid cross-feeding and B-vitamin metabolic exchange between B. sinica and other heterotrophs seemed to be enhanced with acetate addition, contributing to a reduction in metabolic energy cost to the whole community. Our work not only clarified the mechanism underlying discrepant responses of different anammox species to acetate, but also suggests a possible strategy for obtaining higher nitrogen removal rates in wastewater treatment under low C/N ratio.

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Section: Lc-ms-based Metabolomic Profiling and Quantitation Analysismentioning

confidence: 99%

Discrepant gene functional potential and cross-feedings of anammox bacteria Ca. Jettenia caeni and Ca. Brocadia sinica in response to acetate

et al. 2019

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“…Two identical sequencing batch reactors (SBRs) (R-control and R-DSF), with a working volume of 1.0 L were operated for 95 days. Both SBRs were inoculated with 0.29 gVSS L -1 anammox consortia [12].…”

Section: Reactor Operationmentioning

confidence: 99%

“…Previous studies demonstrated that digestive enzymes [7,8], polymeric substances [9], chelating compounds [10], or amino acids [11] could be secreted into extracellular environments as public goods. Extracellular polymeric substances (EPS) are critical for wastewater treatment sludge because of their role in aggregation and biomass tolerance against inhibitors [5,12]. Studies have suggested that amino acid auxotrophy potentially shapes the community composition [13,14].…”

Section: Introductionmentioning

confidence: 99%

“…The existence of the genes coding for QS molecules in anammox community was first evidenced by Strous et al [22] while deciphering the metagenome. Signal messenger bis-(3'-5')-cyclic dimeric guanosine monophosphate (c-di-GMP) was also found in anammox culture [23], and it acts as an important regulator for anammox consortia against environmental stresses [12]. Importantly, according to a recent report, among the multiple QS signal systems, diffusible signal factor (DSF) signaling plays a key role in the anammox community during anammox reactor start-up [24].…”

Section: Introductionmentioning

confidence: 99%

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Deciphering bacterial interactions via DSF-regulated public goods in an anammox community

Guo

Zhao

Tang

et al. 2019

Preprint

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Background: Bacterial interaction and communication via quorum sensing (QS) received extensively attention, as it can coordinate bacterial behavior and activity through the QS signal molecules in microbial community. Though the exchange of public goods regulated by QS have been explored in pure culture, how signal sense, transmit, and affect the social traits through regulating public goods in complex communities remains unclear. Results: The levels of public goods (e.g. extracellular polymeric substances (EPS) and amino acids) changed significantly when exogenous diffusion signal factor (DSF), a kind of QS molecules, was added. Approaches involving meta-omics and hierarchical signalling network construction give insight into that anammox species ( Jettenia caeni , AMX1) and Proteobacteria -affiliated bacterium (PRO1) can sense and transit DSF signals, thus directly regulating the production and exchange of public goods via the secondary messenger c-di-GMP regulator, Clp. In detail, these two kinds of species can supply more costly amino acids for DSF-Secretor species (like AMX2, CFX1, CFX3, and PRO4) after sensing DSF. Meanwhile, DSF-Secretor species encoded diverse genes involved in hydrolysis of extracellular protein and carbohydrate and genes involved in transportation of peptides and sugars. The exogenous DSF-inducement also leads to the high expression of these genes, which indicated DSF-Secretor species helped anammox bacteria scavenge extracellular detritus. This process can be considered as a feedback of public goods supply by anammox bacteria, as this process contributed to create a suitable environment for anammox bacteria growth. Namely, DSF can bridge bacterial interactions through regulating public goods. Furthermore, the trade-off induces discrepant metabolic loads of different microbial clusters and community succession. It illustrated the potential to artificially alleviate metabolic loads and thus increase proliferation rate for certain bacteria through QS. Conclusions: DSF can bridge interactions of anammox bacteria and DSF-Secretor species through regulating production and exchange of public goods using Clp regulator. Deciphering microbial interactions via QS provides insights for understanding the molecular evolution of QS in microbial community.

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“…In this reaction, SAM supplies the amino group and acyl-ACP provides the acyl group. These molecules are used by Gram-negative bacteria as common signaling molecules and are involved in the regulation of biofilm formation and other properties of the bacteria (Galloway et al 2012; Guo et al 2017). When the concentration of an AHL reaches a threshold as a result of an increase in bacterial cell density, members of the LuxR protein family, which consist of the AHL receptors, would bind to the AHL to regulate the expression of numerous genes involved in various processes, including bioluminescence, pigment and/or antibiotic production, and virulence (Waters and Bassler 2005).…”

Section: Introductionmentioning

confidence: 99%

Effect of the luxI/R gene on AHL-signaling molecules and QS regulatory mechanism in Hafnia alvei H4

Zhang

Zhu

et al. 2019

AMB Expr

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Hafnia alvei H4 is a bacterium subject to regulation by a N-acyl-l-homoserine lactone (AHL)-mediated quorum sensing system and is closely related to the corruption of instant sea cucumber. Studying the effect of Hafnia alvei H4 quorum sensing regulatory genes on AHLs is necessary for the quality and preservation of instant sea cucumber. In this study, the draft genome of H. alvei H4, which comprises a single chromosome of 4,687,151 bp, was sequenced and analyzed and the types of AHLs were analyzed employing thin-layer chromatography (TLC) and high resolution triple quadrupole liquid chromatography/mass spectrometry (LC/MS). Then the wild-type strain of H. alvei H4 and the luxI/R double mutant (ΔluxIR) were compared by transcriptome sequencing (RNA-seq). The results indicate that the incomplete genome sequence revealed the presence of one quorum-sensing (QS) gene set, designated as lasI/expR. Three major AHLs, N-hexanoyl-l-homoserine lactone (C6-HSL), N-butyryl-l-homoserine lactone (C4-HSL), and N-(3-oxo-octanoyl)-l-homoserine lactone (3-oxo-C8-HSL) were found, with C6-HSL being the most abundant. C6-HSL was not detected in the culture of the luxI mutant (ΔluxI) and higher levels of C4-HSL was found in the culture of the luxR mutant (ΔluxR), which suggested that the luxR gene may have a positive effect on C4-HSL production. It was also found that AHL and QS genes are closely related in the absence of luxIR double deletion. The results of this study can further elucidate at the genetic level that luxI and luxR genes are involved in the regulation of AHL.

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Insight into c-di-GMP Regulation in Anammox Aggregation in Response to Alternating Feed Loadings

Cited by 89 publications

References 53 publications

Discrepant gene functional potential and cross-feedings of anammox bacteria Ca. Jettenia caeni and Ca. Brocadia sinica in response to acetate

Discrepant gene functional potential and cross-feedings of anammox bacteria Ca. Jettenia caeni and Ca. Brocadia sinica in response to acetate

Deciphering bacterial interactions via DSF-regulated public goods in an anammox community

Effect of the luxI/R gene on AHL-signaling molecules and QS regulatory mechanism in Hafnia alvei H4

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