Insight into cellular proteome of Lolium multiflorum/Festuca arundinacea introgression forms to decipher crucial mechanisms of cold acclimation in forage grasses

Augustyniak, Adam; Perlikowski, Dawid; Rapacz, Marcin; Kościelniak, Janusz; Kosmala, Arkadiusz

doi:10.1016/j.plantsci.2018.04.002

Cited by 23 publications

(34 citation statements)

References 44 publications

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“…However, we assumed here that disturbances in leaf cellular metabolism of the LDT form could result in death after stress termination and/or that adaptations in leaf cellular metabolism of the HDT form could result in its survival advantage. L. multiflorum / F. arundinacea introgression forms, with the selected components of stress tolerance transferred from F. arundinacea to the L. multiflorum genomic background, drought tolerance [ 32 , 40 , 46 , 47 ], and frost tolerance [ 48 , 49 , 50 ], have been proved to be valuable tools to dissect these complex traits into the different components present in the different introgression lines and further to analyze these components in detail. Thus, herein we applied another pair of introgression forms, which could serve as useful plant models to identify yet unrecognized cellular components involved in the mechanisms of drought tolerance in forage grasses.…”

Section: Discussionmentioning

confidence: 99%

Adjustment of Photosynthetic and Antioxidant Activities to Water Deficit Is Crucial in the Drought Tolerance of Lolium multiflorum/Festuca arundinacea Introgression Forms

Lechowicz

Pawłowicz

Perlikowski

et al. 2020

IJMS

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Lolium multiflorum/Festuca arundinacea introgression forms have been proved several times to be good models to identify key components of grass metabolism involved in the mechanisms of tolerance to water deficit. Here, for the first time, a relationship between photosynthetic and antioxidant capacities with respect to drought tolerance of these forms was analyzed in detail. Two closely related L. multiflorum/F. arundinacea introgression forms distinct in their ability to re-grow after cessation of prolonged water deficit in the field were selected and subjected to short-term drought in pots to dissect precisely mechanisms of drought tolerance in this group of plants. The studies revealed that the form with higher drought tolerance was characterized by earlier and higher accumulation of abscisic acid, more stable cellular membranes, and more balanced reactive oxygen species metabolism associated with a higher capacity of the antioxidant system under drought conditions. On the other hand, both introgression forms revealed the same levels of stomatal conductance, CO2 assimilation, and consequently, intrinsic water use efficiency under drought and recovery conditions. However, simultaneous higher adjustment of the Calvin cycle to water deficit and reduced CO2 availability, with respect to the accumulation and activity of plastid fructose-1,6-bisphosphate aldolase, were clearly visible in the form with higher drought tolerance.

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Section: Discussionmentioning

confidence: 99%

Adjustment of Photosynthetic and Antioxidant Activities to Water Deficit Is Crucial in the Drought Tolerance of Lolium multiflorum/Festuca arundinacea Introgression Forms

Lechowicz

Pawłowicz

Perlikowski

et al. 2020

IJMS

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“… Perlikowski et al, 2014 , 2016 ) and abiotic stresses (e.g. Augustyniak et al, 2018 ). The protein identification in the selected spots was performed in the Mass Spectrometry Lab, Institute of Biochemistry and Biophysics, Polish Academy of Sciences (Warsaw, Poland).…”

Section: Methodsmentioning

confidence: 99%

Identification of Proteomic Components Associated with Resistance to Fusarium Head Blight in Rye

et al. 2019

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“…As demonstrated earlier, F. arundinacea (tall fescue) was shown to be a valuable source of genes governing abiotic and biotic stress tolerance to be transferred to closely related, high forage quality L. multiflorum (Italian ryegrass), with a rather limited capacity to withstand these environmental stress conditions. Applications of the research addressing L. multiflorum / F. arundinacea introgression forms, with the selected components of stress tolerance transferred from F. arundinacea into L. multiflorum genomic background (drought tolerance [ 34 , 35 , 36 , 37 ], and freezing tolerance [ 32 , 38 , 39 ]) allowed us to dissect these complex traits into their different components present in different introgression lines, and to further analyze these components separately and in detail.…”

Section: Introductionmentioning

confidence: 99%

“…Augustyniak et al (2018) [ 38 ] suggested the steering role of photosynthetic performance in the process of CA in L. multiflorum / F. arundinacea introgression forms. The photochemical mechanism of photosynthetic adjustment, associated with the acclimation of photosynthetic apparatus to low temperature conditions in the high freezing tolerant (HFT) introgression form, was shown to be crucial to prevent the possible photoinhibition of photosynthesis under cold conditions.…”

Section: Introductionmentioning

confidence: 99%

Freezing Tolerance of Lolium multiflorum/Festuca arundinacea Introgression Forms is Associated with the High Activity of Antioxidant System and Adjustment of Photosynthetic Activity under Cold Acclimation

Augustyniak

Pawłowicz

Lechowicz

et al. 2020

IJMS

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Though winter-hardiness is a complex trait, freezing tolerance was proved to be its main component. Species from temperate regions acquire tolerance to freezing in a process of cold acclimation, which is associated with the exposure of plants to low but non-freezing temperatures. However, mechanisms of cold acclimation in Lolium-Festuca grasses, important for forage production in Europe, have not been fully recognized. Thus, two L. multiflorum/F. arundinacea introgression forms with distinct freezing tolerance were used herein as models in the comprehensive research to dissect these mechanisms in that group of plants. The work was focused on: (i) analysis of cellular membranes’ integrity; (ii) analysis of plant photosynthetic capacity (chlorophyll fluorescence; gas exchange; gene expression, protein accumulation, and activity of selected enzymes of the Calvin cycle); (iii) analysis of plant antioxidant capacity (reactive oxygen species generation; gene expression, protein accumulation, and activity of selected enzymes); and (iv) analysis of Cor14b accumulation, under cold acclimation. The more freezing tolerant introgression form revealed a higher integrity of membranes, an ability to cold acclimate its photosynthetic apparatus and higher water use efficiency after three weeks of cold acclimation, as well as a higher capacity of the antioxidant system and a lower content of reactive oxygen species in low temperature.

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Insight into cellular proteome of Lolium multiflorum/Festuca arundinacea introgression forms to decipher crucial mechanisms of cold acclimation in forage grasses

Cited by 23 publications

References 44 publications

Adjustment of Photosynthetic and Antioxidant Activities to Water Deficit Is Crucial in the Drought Tolerance of Lolium multiflorum/Festuca arundinacea Introgression Forms

Adjustment of Photosynthetic and Antioxidant Activities to Water Deficit Is Crucial in the Drought Tolerance of Lolium multiflorum/Festuca arundinacea Introgression Forms

Identification of Proteomic Components Associated with Resistance to Fusarium Head Blight in Rye

Freezing Tolerance of Lolium multiflorum/Festuca arundinacea Introgression Forms is Associated with the High Activity of Antioxidant System and Adjustment of Photosynthetic Activity under Cold Acclimation

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