Insight into metabolism pathways of pesticide fomesafen in rice: Reducing cropping and environmental risks

Chen, Zhao Jie; Qiao, Yu Xin; Zhang, Nan; Liu, Jintong; Yang, Hong

doi:10.1016/j.envpol.2021.117128

Cited by 33 publications

(28 citation statements)

References 39 publications

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“…In particular, DU-induced LOC_Os10g38340 was reported to play a vital role in regulating the detoxification process of fenoxaprop- p -ethyl in rice . Another extremely upregulated GST gene by DU, LOC_Os10g38470 could grant a broad-spectrum resistance in rice to chemical stresses, such as pesticide fomesafen and atrazine . Recently, other types of Phase II reactions have been discovered to play an important role in the metabolism of the herbicide, such as acetylation, amino acid-based condensation, methylation, and sulfonation, whose the candidate regulatory genes including acetyltransferase, acyl-CoA synthase, methyltransferase, and sulfotransferase were identified in DU-exposed rice (Figure ).…”

Section: Resultsmentioning

confidence: 99%

“…34 These metabolic processes were divided into four phases, according to the types of enzyme-catalyzed reactions. 35 In Phase I, cytochrome P450s, hydrolase, esterase, and monooxygenase are responsible for the dealkylation, hydroxylation, and dehalogenation of intermediates in plants, which is considered to be the first step of xenobiotic detoxification. 36 Plant cytochrome P450s belonging to the largest family of enzymatic proteins have been found to participate in mediating the metabolism of xenobiotics or herbicides.…”

Section: Effect Of Du On the Activities Of Antioxidant Enzymes And Th...mentioning

confidence: 99%

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Molecular Responses and Degradation Mechanisms of the Herbicide Diuron in Rice Crops

Wang

Zhang

Yuan

et al. 2022

J. Agric. Food Chem.

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Diuron [DU; 3-(3,4-dichlorophenyl)-1,1-dimethylurea], a widely used herbicide for weed control, arouses ecological and health risks due to its environment persistence. Our findings revealed that DU at 0.125–2.0 mg L–1 caused oxidative damage to rice. RNA-sequencing profiles disclosed a globally genetic expression landscape of rice under DU treatment. DU mediated downregulated gene encoding photosynthesis and biosynthesis of protein, fatty acid, and carbohydrate. Conversely, it induced the upregulation of numerous genes involved in xenobiotic metabolism, detoxification, and anti-oxidation. Furthermore, 15 DU metabolites produced by metabolic genes were identified, 7 of which include two Phase I-based and 5 Phase II-based derivatives, were reported for the first time. The changes of resistance-related phytohormones, like JA, ABA, and SA, in terms of their contents and molecular-regulated signaling pathways positively responded to DU stress. Our work provides a molecular-scale perspective on the response of rice to DU toxicity and clarifies the biotransformation and degradation fate of DU in rice crops.

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Section: Resultsmentioning

confidence: 99%

Section: Effect Of Du On the Activities Of Antioxidant Enzymes And Th...mentioning

confidence: 99%

Molecular Responses and Degradation Mechanisms of the Herbicide Diuron in Rice Crops

Wang

Zhang

Yuan

et al. 2022

J. Agric. Food Chem.

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“…Due to the relatively high persistence and mobility of IPU in farmland, it is important to find a green, safe, and effective way to minimize the residual IPU in both the environment and crops. Previous studies demonstrate that some CYP family protein genes are involved in the regulation of metabolism and degradation, thus enhancing plant or crop resistance to pesticides. ,,,,, Several CYP members were reported to actively facilitate IPU oxidation and metabolism in arabidopsis, tobacco, and potato, − yet the biological roles of these regulators in crops toward IPU metabolism and detoxification remained elusive. In this work, we provided new evidence that a CYP gene CYP76C6 is able to detoxify and metabolize IPU residues in rice.…”

Section: Discussionmentioning

confidence: 99%

Expression of CYP76C6 Facilitates Isoproturon Metabolism and Detoxification in Rice

Zhai

Chen

Liu

et al. 2022

J. Agric. Food Chem.

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Agricultural chemical residues in farmland and crops is one of the serious public issues that constantly threatens crop production, food security, and human health. Understanding their decay mechanism in crops for accelerating their degradative metabolism is important. In this study, a rice uncharacterized cytochrome P450 gene encoding CYP76C6 was functionally identified in rice exposed to isoproturon (IPU). To verify the role of CYP76C6 in rice resistance to IPU toxicity, CYP76C6 overexpression (OEs) and knockout mutant rice by CRISPR/Cas9 were generated through genetic transformation and gene-editing technologies. Assessment of growth and physiological responses revealed that the growth of OE lines was improved, the IPU-induced cellular damage was attenuated, and IPU accumulation was significantly repressed, whereas the Cas9 lines displayed a contrasting phenotype compared to the wild-type. Both relative contents of IPU metabolites and conjugates in OE lines were reduced and those in Cas9 line were increased, suggesting that CYP76C6 plays a critical role in IPU degradation. Our study unveils a new regulator, together with its mechanism for IPU decay in rice crops, which will be used in reality to reduce environmental risks in food safety and human health.

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“…However, they differ in the existence of notable enzymes such as cytochrome P450 and glutathione-S-transferase (GST). The presence of these enzymes particularly in paddy is of great importance to rapidly metabolize the herbicides into inactive products [13,18]. Therefore, ACCase herbicides consider this advantage as the basis of selectivity between crops and weeds [19].…”

Section: Introductionmentioning

confidence: 99%

Searching of Novel Herbicides for Paddy Field Weed Management—A Case Study with Acetyl-CoA Carboxylase

Antony

Ramanathan

2022

Agronomy

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Weed management is the major biological constraint in paddy (Oryza sativa L.) producing areas. Predominantly, barnyard grass (Echinochloa crus-galli) is a rice-mimicking weed that causes 57% of yield loss in rice production. Conventionally, herbicides are the site-specific weed inhibitors often used to suppress E. crus-galli growth. Acetyl-CoA carboxylase (ACCase) is an important target for developing novel herbicides with remarkable selectivity against gramineous weeds. Notably, fenoxaprop-P-ethyl (FPPE) is a selective ACCase herbicide extensively used in paddy fields to inhibit barnyard grass. However, prolonged use of FPPE herbicide elicits phytotoxicity in cultivated rice and herbicide resistance in weeds. Recently, phytotoxins are emerging as an alternative to commercial herbicides with safer environmental profiles. Nevertheless, discovering natural herbicides through in vivo and in vitro techniques is time-consuming and expensive. Therefore, high-end computational screening strategies including Tanimoto similarity, docking, binding free energy, and herbicide-likeness were used to pinpoint the lead molecule. Finally, molecular dynamics and MM/PBSA calculations were employed to validate the binding kinetics of the hit compound. Indeed, sinigrin was identified as a promising phytotoxic inhibitor against the ACCase enzyme. The findings of our study were well correlated with the existing experimental results. Overall, the current work will aid in the development of commercializing phytotoxin herbicides in foreseeable future.

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Insight into metabolism pathways of pesticide fomesafen in rice: Reducing cropping and environmental risks

Cited by 33 publications

References 39 publications

Molecular Responses and Degradation Mechanisms of the Herbicide Diuron in Rice Crops

Molecular Responses and Degradation Mechanisms of the Herbicide Diuron in Rice Crops

Expression of CYP76C6 Facilitates Isoproturon Metabolism and Detoxification in Rice

Searching of Novel Herbicides for Paddy Field Weed Management—A Case Study with Acetyl-CoA Carboxylase

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