Insight into the three-dimensional structure of maize chlorotic mottle virus revealed by Cryo-EM single particle analysis

Wang, Chunyan; Zhang, Qinfen; Gao, Yuanzhu; Zhou, Xueping; Ji, Gang; Huang, Xiaojun; Hong, Jian; Zhang, Chuan‐Xi

doi:10.1016/j.virol.2015.07.014

Cited by 7 publications

(6 citation statements)

References 37 publications

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“…Interestingly, the S domain has a higher similarity to some plant virus shell proteins compared to that of the FHV which is thought to be the representative virus of Alphanodavirus . The Z score for the comparison to carnation mottle virus [ 40 ] and maize chlorotic mottle virus [ 41 ] is 8.0 and 5.6, respectively. In the reported alphanodaviruses, there is no obvious protrusion.…”

Section: Discussionmentioning

confidence: 99%

Structural analysis and insertion study reveal the ideal sites for surface displaying foreign peptides on a betanodavirus-like particle

Xie¹,

Li²,

Gao³

et al. 2016

Vet Res

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Betanodavirus infection causes fatal disease of viral nervous necrosis in many cultured marine and freshwater fish worldwide and the virus-like particles (VLP) are effective vaccines against betanodavirus. But vaccine and viral vector designs of betanodavirus VLP based on their structures remain lacking. Here, the three-dimensional structure of orange-spotted grouper nervous necrosis virus (OGNNV) VLP (RBS) at 3.9 Å reveals the organization of capsid proteins (CP). Based on the structural results, seven putative important sites were selected to genetically insert a 6× histidine (His)-tag for VLP formation screen, resulting in four His-tagged VLP (HV) at positions N-terminus, Ala220, Pro292 and C-terminus. The His-tags of N-terminal HV (NHV) were concealed inside virions while those of 220HV and C-terminal HV (CHV) were displayed at the outer surface. NHV, 220HV and CHV maintained the same cell entry ability as RBS in the Asian sea bass (SB) cell line, indicating that their similar surface structures can be recognized by the cellular entry receptor(s). For application of vaccine design, chromatography-purified CHV could provoke NNV-specific antibody responses as strong as those of RBS in a sea bass immunization assay. Furthermore, in carrying capacity assays, N-terminus and Ala220 can only carry short peptides and C-terminus can even accommodate large protein such as GFP to generate fluorescent VLP (CGV). For application of a viral vector, CGV could be real-time visualized to enter SB cells in invasion study. All the results confirmed that the C-terminus of CP is a suitable site to accommodate foreign peptides for vaccine design and viral vector development.

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Section: Discussionmentioning

confidence: 99%

Structural analysis and insertion study reveal the ideal sites for surface displaying foreign peptides on a betanodavirus-like particle

Xie¹,

Li²,

Gao³

et al. 2016

Vet Res

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“…Two thousand adult wasps were ground, and the virus particles were purified as described previously [73]. The ground samples were dissolved in 0.1 M phosphate buffer (pH 7.0).…”

Section: Methodsmentioning

confidence: 99%

A novel negative-stranded RNA virus mediates sex ratio in its parasitoid host

et al. 2017

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Parasitoid wasps are important natural enemies of arthropod hosts in natural and agricultural ecosystems and are often associated with viruses or virion-like particles. Here, we report a novel negative-stranded RNA virus from a parasitoid wasp (Pteromalus puparum). The complete viral genome is 12,230 nucleotides in length, containing five non-overlapping, linearly arranged open reading frames. Phylogenetically, the virus clusters with and is a novel member of the mononegaviral family Nyamiviridae, here designated as Pteromalus puparum negative-strand RNA virus 1 (PpNSRV-1). PpNSRV-1 is present in various tissues and life stages of the parasitoid wasp, and is transmitted vertically through infected females and males. Virus infections in field populations of P. puparum wasps ranged from 16.7 to 37.5%, without linearly correlating with temperature. PpNSRV-1 increased adult longevity and impaired several fitness parameters of the wasp, but had no influence on successful parasitism. Strikingly, PpNSRV-1 mediated the offspring sex ratio by decreasing female offspring numbers. RNA interference knockdown of virus open reading frame I eliminated these PpNSRV-1-induced effects. Thus, we infer that PpNSRV-1 has complex effects on its insect host including sex ratio distortion towards males, as well as possible mutualistic benefits through increasing wasp longevity.

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“…It is a single stranded RNA virus, 4.4 kb with icosahedral shape. 1 The virus has a smooth surface 2 and has a thermal inactivation point of 80-85°C, and can maintain its infectivity at 20°C for 33 days. 3 The virus causes maize lethal necrosis (MLN) disease in the presence of a cereal-infecting potyvirus.…”

Section: Introductionmentioning

confidence: 99%

Infectivity of Maize Chlorotic Mottle Virus from Contaminated Maize Seeds

Kimani,

Muriki,

Micheni

et al. 2023

Curr Agri Res Jour

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Seeds have been identified as major sources of introduction and spread of pathogens, with viruses being detected in the seed and also on the seed coat. In this study, the infectivity of Maize chlorotic mottle virus (MCMV) through seeds was investigated. Maize seeds that had tested positive for MCMV previously using double antibody enzyme-linked immunosorbent assay (DAS-ELISA) and real-time reverse transcription polymerase chain reaction (real time RT-PCR) were obtained from various sources. The seeds were soaked in phosphate buffer overnight and the solution used to inoculate maize seedlings. The whole seed was also ground and mixed with the buffer and used for inoculation of seedlings by hand rubbing. Visible MCMV symptoms were observed on less than 2% of the 547 seedlings inoculated with the seed soak and seed extract from contaminated seed 28 days after inoculation and this was confirmed using DAS-ELISA. Use of real time reverse transcription polymerase chain reaction revealed infectivity of MCMV from one of the seed sources used. The mean cycle threshold (Ct) values of samples that showed infectivity ranged from 28.21 to 29.40 cycles. The means were significantly different (P<0.001) from the other samples tested, the healthy and negative controls. When compared to seedlings inoculated with MCMV-infected leaf sap, there was visible development of symptoms associated with MCMV infection, with a severity score of three and Ct values as low as 11.53. The results show evidence of infection of MCMV on maize seedlings caused by virus present in seed extract. Despite rare occurrence of infectivity, the presence of viable virus may cause spread of the virus in the field, leading to development of maize lethal necrosis disease where a cereal potyvirus is present.

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Insight into the three-dimensional structure of maize chlorotic mottle virus revealed by Cryo-EM single particle analysis

Cited by 7 publications

References 37 publications

Structural analysis and insertion study reveal the ideal sites for surface displaying foreign peptides on a betanodavirus-like particle

Structural analysis and insertion study reveal the ideal sites for surface displaying foreign peptides on a betanodavirus-like particle

A novel negative-stranded RNA virus mediates sex ratio in its parasitoid host

Infectivity of Maize Chlorotic Mottle Virus from Contaminated Maize Seeds

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