Insight of Molecular Prevalence on Antibiotic Sensitive <i>H. pylori</i> Biotypes from Apparently Healthy and Clinical Illness Felines and Sheep

Abstract

Helicobacter pylori is gram-negative bacteria may transmit through human food chain causing serious health problems in humans worldwide upon misusing antibiotic therapy for animals. Aim of the study represent the incidence of variants biotypes H. pylori susceptible to antibiotic in apparently healthy or clinical illness felines and sheep through amplification of 16srRNA. Four stomachs of 3 apparently healthy and diarrheal feline and two stool of constipated and panleukopenia felines, in addition to five gastric sheep from 2 normal and 3 congested plus one milk, selected from 52 felines and 83 sheep, respectively based on traditional cultivation and biochemical differentiation in total twelve H. pylori isolates that confirmed by amplification of 16srRNA, not being recognized by v3-v4 primer as nitrate gram negative bacteria. H. pylori isolates were grouped upon urease and nitrate reduction reaction in total percent 50% for each of weak and strong urease biotypes, including 33.3% & 66.6% for (+ve) or (-ve) nitrate reductive biotypes, respectively. Sensitivity of these biotypes was determined against fourteen antibiotic discs by antimicrobial susceptibility test to find highest sensitivity non-producing nitrate biotypes from felines is (87.5%), lesser than sheep (100%) but almost positive nitrate reductive isolates is less susceptible in percent 25%. Moderate sensitivity of weak urease biotypes represents 50% against amikacin, opposite to strong urease isolates (33.3%). Highest sensitivity strong urease biotypes show 83.3% against clarithromycin and levofloxacin, compared to weak urease biotypes 50 & 66.6%, respectively. Bio-typing H. pylori is preferable for programming eradication in molecular surveying normal or clinical illness animals.