Insight of the optical property of laccase during polymerics formation for application in real-time biosensing

Wang, Yan; Wu, Na; Chen, Zhihui

doi:10.1007/s10853-021-06186-5

Cited by 3 publications

(3 citation statements)

References 45 publications

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“…Plant and animal laccases are difficult to obtain as crystals due to their low abundance and complex purification methods. However, despite their wide taxonomic distribution and substrate diversity, the molecular structure of laccase has proven to be common to all polyvalent copper oxidases [32,33]. Laccases' domains and amino acid sequences share similarities with those of all multivalent copper oxidases.…”

Section: Main Laccase Types and Their Structurementioning

confidence: 99%

“…Most laccases have important optical properties due to the presence of copper atoms in the catalytic center, as stated earlier. Most of the kinetic and spectroscopic studies on laccases are summarized in a book by Messerschmidt [45] as well as in several reviews [33,46]. As already mentioned, laccases are also categorized by their optical properties: blue, yellow, and white laccases.…”

Section: Uv-vis Absorption Spectroscopymentioning

confidence: 99%

“…In the case of yellow and white laccase, the FTIR spectra present similar contributions, but the bands are characterized by different intensities, thus suggesting different relative weights of the various components. Fluorescence spectroscopy has been successfully adopted by several authors for characterizing different aspects of laccases [33,70,71]. In recent years, interest in the application of this technique has particularly been addressed to fungal and bacterial laccases [72,73].…”

Section: Fourier Transform Infrared (Ft-ir) Spectroscopymentioning

confidence: 99%

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Optical Properties of Laccases and Their Use for Phenolic Compound Detection and Quantification: A Brief Review

Conigliaro,

Portaccio,

Lepore

et al. 2023

Applied Sciences

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Phenolic compounds (PheCs) are particularly relevant in many different frameworks due to their pro-oxidant and antioxidant activities. In fact, on the one hand, they are considered very dangerous pro-oxidant agents that can be present in the environment as pollutants in wastewater and soil from different industrial and agricultural industries. On the other hand, the antioxidant influence of PheCs available in natural products (including foods) is nowadays considered essential for preserving human health. Conventional techniques for detecting PheCs present some disadvantages, such as requiring expensive instrumentation and expert users and not allowing in situ measurements. This is the reason why there is a high interest in the development of simple, sensitive, specific, and accurate sensing methods for PheCs. Enzymes are often used for this purpose, and laccases with unique optical properties are adopted as bio-elements for sensing schemes. The present paper aims to revise the optical properties of laccases and their use for developing PheC detection and quantification methods used in different fields such as environment monitoring, food characterization and medical applications. In particular, the results offered by UV, visible and infrared absorption, fluorescence, Raman, and surface-enhanced Raman spectroscopy (SERS) have been considered. The enzymatic biosensing devices developed using the related optical signals have been reported, and a comparison of their performances has carried out. A brief description of the main characteristics of laccase and phenols is also given.

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Section: Main Laccase Types and Their Structurementioning

confidence: 99%

Section: Uv-vis Absorption Spectroscopymentioning

confidence: 99%

Section: Fourier Transform Infrared (Ft-ir) Spectroscopymentioning

confidence: 99%

See 1 more Smart Citation

Optical Properties of Laccases and Their Use for Phenolic Compound Detection and Quantification: A Brief Review

Conigliaro,

Portaccio,

Lepore

et al. 2023

Applied Sciences

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Enzymatic route for selective glycerol oxidation using covalently immobilized laccases

Bîtcan

Petrovici

Pellis

et al. 2023

Enzyme and Microbial Technology

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Micro-displacement detection of nanofluidic fluorescent particles based on waveguide-concentric ring resonator model

Li¹,

Chen²,

Feng³

et al. 2022

Acta Phys. Sin.

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Dynamic tracking and detection of nanoparticles in micro-nanofluids have always been a challenging and demanding work. In this work, we propose a waveguide-concentric ring resonator integrated optical platform, based on the waveguide-concentric ring resonator. The change of the fluorescence power intensity output by the cavity coupling structure is used to realize the micro-displacement detection of nanoparticles in the micro-nano fluid. Because the ring micro-resonator has the characteristics of high Q and is sensitivity to the surrounding environment, the sensitivity of the device is greatly improved. The finite-difference time domain method has been carried out on the study of parameters such as the polarization state of the fluorescence and the distance between the two ring resonators. The double-peak changes of the fluorescence output power can be used to detect the displacement of the nanoparticles with high precision. Based on the synchronization of the double-peak changes, the detection can reduce the influence of environmental noise and improve the detection accuracy. The numerical simulation results also confirm that this method can measure the micro-displacement of nanoparticles in nanofluids in the range of 0-1000nmproviding new directions and ideas.

show abstract

Insight of the optical property of laccase during polymerics formation for application in real-time biosensing

Cited by 3 publications

References 45 publications

Optical Properties of Laccases and Their Use for Phenolic Compound Detection and Quantification: A Brief Review

Optical Properties of Laccases and Their Use for Phenolic Compound Detection and Quantification: A Brief Review

Enzymatic route for selective glycerol oxidation using covalently immobilized laccases

Micro-displacement detection of nanofluidic fluorescent particles based on waveguide-concentric ring resonator model

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