“…In each TCCS, 4-kg of sediment samples were placed in 8000 ml of altered and sterilized in situ seawater. One PVC frame, which hold 10 microscope slides, was immersed in seawater at a depth of 10 cm from the sediment layer for each TCCS (Gui et al, 2022;Song et al, 2022). At each temperature event, 10 slide samples were transferred to petri dishes containing sterilized seawater at intervals of 3, 5, 7, 10, 14, and 21 days after exposure.…”