Insights from a novel tumor model: Indications for a quantitative link between tumor growth and invasion

Deisboeck, Thomas S.; Mansury, Yuri; Guiot, Caterina; Degiorgis, Piero Giorgio; Delsanto, Pier Paolo

doi:10.1016/j.mehy.2005.04.014

Cited by 24 publications

(25 citation statements)

References 11 publications

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“…For instance, one could hypothesize that genetic and epigenetic profiles which increase the tumor's invasive behavior are selected for at a relatively early stage -a process that would have implications both for diagnostics and therapeutics alike. Furthermore, we argue that this surface-expansion due to nourishment requirements complements the cell density-and thus largely mechanically-driven trigger, described in detail in Deisboeck et al (2005).…”

Section: Figure 1 Herementioning

confidence: 60%

Does cancer growth depend on surface extension?

et al. 2006

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Section: Figure 1 Herementioning

confidence: 60%

Does cancer growth depend on surface extension?

et al. 2006

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“…As an example, cell density modulates focal adhesion kinase (Fak) activation, a regulator of glioma invasion [19,20]. Deisboeck et al, have suggested that the onset of invasion marks the time point when the tumor's cell density reaches a compaction maximum [21]. One difference between cells within invasive tumor cell islets and those in the bulk tumor we sampled with LCMD was relative local tumor cell density.…”

Section: Resultsmentioning

confidence: 99%

Density-Dependent Regulation of Glioma Cell Proliferation and Invasion Mediated by miR-9

Katakowski

Charteris

Chopp

et al. 2016

Cancer Microenvironment

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The phenotypic axis of invasion and proliferation in malignant glioma cells is a well-documented phenomenon. Invasive glioma cells exhibit a decreased proliferation rate and a resistance to apoptosis, and invasive tumor cells dispersed in brain subsequently revert to proliferation and contribute to secondary tumor formation. One miRNA can affect dozens of mRNAs, and some miRNAs are potent oncogenes. Multiple miRNAs are implicated in glioma malignancy, and several of which have been identified to regulate tumor cell motility and division. Using rat 9 L gliosarcoma and human U87 glioblastoma cell lines, we investigated miRNAs associated with the switch between glioma cell invasion and proliferation. Using micro-dissection of 9 L glioma tumor xenografts in rat brain, we identified disparate expression of miR-9 between cells within the periphery of the primary tumor, and those comprising tumor islets within the invasive zone. Modifying miR-9 expression in in vitro assays, we report that miR-9 controls the axis of glioma cell invasion/proliferation, and that its contribution to invasion or proliferation is biphasic and dependent upon local tumor cell density. In addition, immunohistochemistry revealed elevated hypoxia inducible factor 1 alpha (HIF-1α) in the invasive zone as compared to the primary tumor periphery. We also found that hypoxia promotes miR-9 expression in glioma cells. Based upon these findings, we propose a hypothesis for the contribution of miR-9 to the dynamics glioma invasion and satellite tumor formation in brain adjacent to tumor.

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“…The microscopic histological images shown in our previous work [27] also indicate the "island" structure and high density of cancerous cell. The higher cell density in cancerous prostate tissue [29,33,34] gives the molecules less "free" rotation space [10,29,34]. In addition, the dye crowds more in cancerous tissue due to higher adsorption levels of bound Cytate as shown by the larger emission intensity in Fig.…”

Section: B Time-resolved Fluorescence Polarization Anisotropy Of Cytmentioning

confidence: 97%

“…First the tumor grows in volume; when it reaches some kind of confining volume, the mechanical pressure increases its cell density. When the tumor cell density exceeds a certain compaction maximum, invasion starts [29,33]. The tumor grading system for prostate cancer includes the well known five Gleason grades, usually denoted as stages 1-5 [34].…”

Section: B Time-resolved Fluorescence Polarization Anisotropy Of Cytmentioning

confidence: 99%

Time-resolved fluorescence polarization dynamics and optical imaging of Cytate: a prostate cancer receptor-targeted contrast agent

Pu¹,

Wang²,

Das³

et al. 2008

Appl. Opt.

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Cypate-octreote peptide analogue conjugate (Cytate) was investigated as a prostate cancer receptortargeted contrast agent. The absorption and fluorescence spectra of Cytate were ranged in the nearinfrared "tissue optical window." Time-resolved investigation of polarization-dependent fluorescence emitted from Cytate in solution as well as in cancerous and normal prostate tissues was conducted. Polarization preservation characteristics of Cytate in solution and tissues were studied. Fluorescence intensity emitted from the Cytate-stained cancerous prostate tissue was found to be much stronger than that from the Cytate-stained normal prostate tissue, indicating more Cytate uptake in the former tissue type. The polarization anisotropy of Cytate contained in cancerous prostate tissue was found to be larger than that in the normal prostate tissue, indicating a larger degree of polarization preservation in Cytate-stained cancerous tissue. The temporal profiles of fluorescence from Cytate solution and from Cytate-stained prostate tissue were fitted using a time-dependent fluorescence depolarization model. The photoluminescence imaging of Cytate-stained cancerous and normal prostate tissues was accomplished, showing the potential of Cytate as a fluorescence marker for prostate cancer detection.

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Insights from a novel tumor model: Indications for a quantitative link between tumor growth and invasion

Abstract: Both the lack of nutrient supply and rising mechanical stress exerted by the microenvironment appear to be able to cause discrepancies between the actual, observed tumor mass and that

Cited by 24 publications

References 11 publications

Does cancer growth depend on surface extension?

Does cancer growth depend on surface extension?

Density-Dependent Regulation of Glioma Cell Proliferation and Invasion Mediated by miR-9

Time-resolved fluorescence polarization dynamics and optical imaging of Cytate: a prostate cancer receptor-targeted contrast agent

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