Insights into Cross-Kingdom Plant Pathogenic Bacteria

Kirzinger, Morgan W. B.; Nadarasah, Geetanchaly; Stavrinides, John

doi:10.3390/genes2040980

Cited by 49 publications

(62 citation statements)

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“…The identification of the PSI-1b system in the clinical strain, P. calida B021323 was notable given previous work showing the plant pathogenic potential of many clinical strains and the animal pathogenic potential of environmental strains (Volksch et al 2009) (Nadarasah and. This discovery reinforces the notion that clinical strains of Pantoea are not necessarily dedicated animal pathogens, but may be plant pathogens or epiphytes that can colonize animal hosts opportunistically (Stavrinides 2009;Stavrinides et al 2010;Kirzinger et al 2011;Nadarasah and Stavrinides 2014).…”

Section: Discussionsupporting

confidence: 73%

Inheritance of Pantoea type III secretion systems through both vertical and horizontal transfer

2015

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The type III secretion system (T3SS) is an extracellular apparatus used by many Gram-negative bacteria to deliver effector proteins directly into plant and animal cells, thereby facilitating host-specific association. Strains of the enterobacterial genus, Pantoea, have been isolated from a wide variety of hosts, including plants, insects, and humans, yet it is unclear whether the T3SS may be involved in these associations. In this study, we use comparative genomics and phylogenetic methods to examine the origin and distribution of T3SSs in 35 sequenced environmental and clinical strains of Pantoea. We began our analysis by examining the distribution of the previously characterized plant cell-specific PSI-1 and animal cell-specific PSI-2 of the plant pathogenic Pantoea stewartii subsp. stewartii DC283 (PstDC283), and showed that both had a somewhat limited distribution. Our analysis, however, identified two variants of a unique plant cell-specific T3SS (PSI-1a and PSI-1b) in six Pantoea strains, including a clinical isolate. Our genome analysis of PstDC283 also identified a third T3SS that we named PSI-3, which has a similar genetic content and organization to the Salmonella, animal cell-specific SPI-2 system. Phylogenetic analysis of all three systems suggests that the PSI-1 system has been inherited vertically, whereas the newly identified PSI-1a and PSI-1b systems have been acquired independently from other genera within the Enterobacteriaceae. PSI-2 appears to have been acquired horizontally as far back as the Erwinia/Pantoea common ancestor, with evidence of more recent horizontal acquisition of the PSI-3 system. Our results suggest that Pantoea is a relatively old plant pathogen that has lost and subsequently regained different plant-associated T3SSs. This work has broad implications for understanding the host-associating capacity of Pantoea strains, and reveals the propensity for Pantoea isolates to exchange pathogenicity determinants with human-pathogenic members of the Enterobacteriaceae.

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Section: Discussionsupporting

confidence: 73%

Inheritance of Pantoea type III secretion systems through both vertical and horizontal transfer

2015

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“…Pantoea and Burkholderia spp. are cross-kingdom pathogens, which infect humans and plants (Kirzinger, Nadarasah and Stavrinides 2011). Several plant-pathogenic bacteria including Xanthomonas albilineans and X. axonopodis pv.…”

Section: Introductionmentioning

confidence: 99%

Behind the lines–actions of bacterial type III effector proteins in plant cells

Büttner¹

2016

FEMS Microbiology Reviews

243

234

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Pathogenicity of most Gram-negative plant-pathogenic bacteria depends on the type III secretion (T3S) system, which translocates bacterial effector proteins into plant cells. Type III effectors modulate plant cellular pathways to the benefit of the pathogen and promote bacterial multiplication. One major virulence function of type III effectors is the suppression of plant innate immunity, which is triggered upon recognition of pathogen-derived molecular patterns by plant receptor proteins. Type III effectors also interfere with additional plant cellular processes including proteasome-dependent protein degradation, phytohormone signaling, the formation of the cytoskeleton, vesicle transport and gene expression. This review summarizes our current knowledge on the molecular functions of type III effector proteins with known plant target molecules. Furthermore, plant defense strategies for the detection of effector protein activities or effector-triggered alterations in plant targets are discussed.

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“…This is consistent with several studies showing that Klebsiella isolates are able to fix nitrogen and live in association with plants (Iniguez et al ., ; Rosenblueth et al ., ). Similarly, strains of the genus Serratia , a human pathogen typically found in the respiratory and urinary tracts, have also been found to be PP (Kirzinger et al ., ). Among the 20 analysed Serratia isolates, 18 are assigned probability values above 0.76 (Supporting Information Table S11 and File S2), with the only exceptions being the two endosymbionts Serratia symbiotica SCc and S. symbiotica Tucson (Lamelas et al ., ).…”

Section: Resultsmentioning

confidence: 97%

Prediction of bacterial associations with plants using a supervised machine‐learning approach

Martínez-García

López‐Solanilla

Ramos

et al. 2016

Environmental Microbiology

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Recent scenarios of fresh produce contamination by human enteric pathogens have resulted in severe food-borne outbreaks, and a new paradigm has emerged stating that some human-associated bacteria can use plants as secondary hosts. As a consequence, there has been growing concern in the scientific community about these interactions that have not yet been elucidated. Since this is a relatively new area, there is a lack of strategies to address the problem of food-borne illnesses due to the ingestion of fruits and vegetables. In the present study, we performed specific genome annotations to train a supervised machine-learning model that allows for the identification of plant-associated bacteria with a precision of ∼93%. The application of our method to approximately 9500 genomes predicted several unknown interactions between well-known human pathogens and plants, and it also confirmed several cases for which evidence has been reported. We observed that factors involved in adhesion, the deconstruction of the plant cell wall and detoxifying activities were highlighted as the most predictive features. The application of our strategy to sequenced strains that are involved in food poisoning can be used as a primary screening tool to determine the possible causes of contaminations.

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Insights into Cross-Kingdom Plant Pathogenic Bacteria

Cited by 49 publications

References 100 publications

Inheritance of Pantoea type III secretion systems through both vertical and horizontal transfer

Inheritance of Pantoea type III secretion systems through both vertical and horizontal transfer

Behind the lines–actions of bacterial type III effector proteins in plant cells

Prediction of bacterial associations with plants using a supervised machine‐learning approach

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