Insights into high temperature pretreatment on cellulase processing of bamboo

Abstract

Bamboo processing was performed with commercial cellulase. The properties of cellulase and the effect of high temperature pretreatment on cellulase hydrolysis of bamboo were investigated. Results indicated that cellulase hydrolysis performed fast and dramatically within 30 minutes, and then gradually reached its balance. It was found that pretreatment played an active role in cellulase processing, which enhanced the saccharification of bamboo and benefited high-molecular-weight lignin degradation and removal. Additionally, a better performance of bamboo processing was achieved under the cellulase concentration of 15IU in total reaction system of 100 ml at 50°C, pH 4.8, together with the high temperature pretreatment of 120°C for 15 minutes

Cutinase promotes dry esterification of cotton cellulose

“…About 200 μL of per sample and 150 μL of DNS solution were incubated in boiling water for 5 min, then diluted by 2.15 mL of distilled water after cooling down using running cold water. And 200 μL of prepared sample were tested at 540 nm …”

“…9 The absorption peak at 1745 cm 21 for cotton treated with active cutinase was significantly stronger (56% of increase in peak area) than that for the control sample. After subtracting the control spectrum, the resulting curve (Figure 4 curve C) showed a prominent peak at 1745 cm 21 . These results independently confirm the MALDI-TOF mass spectra results on the modification of glucose.…”

Characterization of xylanase from Streptomyces sp. FA1 and its application for bamboo hydrolysis

“…Xylanase activity was determined by assaying the liberation of reducing sugar from beechwood xylan according to a modified dinitrosalicylic acid (DNS) method, using xylose solution as a reference [15]. 0.5 mL of appropriately diluted xylanase was incubated with 1.5 mL of 1% (w/v) beechwood xylan solution (dissolved in 50mM buffer, optimum pH) at the optimum temperature for 30 min, and then the reaction was terminated by the addition of 1.5 mL of DNS reagent.…”

Xylanase‐ and cellulose‐aided bioprocessing of bamboo

“…Alternatively, other pretreatments, such as autoclaving, are also known to greatly enhance the enzyme efficiency. Bamboo pretreatments and enzyme accessibility were researched in detail in our previous reports .…”