Insights into <i>in vivo</i> adipocyte differentiation through cell-specific labeling in zebrafish

Lepanto, Paola; Levin-Ferreyra, F.; Koziol, Uriel; Malacrida, Leonel; Badano, José L.

doi:10.1242/bio.058734

Cited by 4 publications

(4 citation statements)

References 61 publications

(100 reference statements)

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“…Although co-culture systems in fish remain to be developed, transgenic lines appeared in zebrafish that helped following in vivo adipocyte differentiation processes or adiposity dynamics ( 309 ), through the imaging of adipocyte- or lipid droplet-specific labels (fabp4-EGFP ( 310 );; plin2-TOMATO ( 311 );). Together with the use of a fasting-refeeding experiment (fabp11-EGFP ( 312 );) or a high fat diet ( 311 ) these lines helped reporting on the metabolic regulations that impact on adipose tissues.…”

Section: New Approaches To Specific Analysis Of Tissue Interactionsmentioning

confidence: 99%

Recent advances in the crosstalk between adipose, muscle and bone tissues in fish

et al. 2023

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Control of tissue metabolism and growth involves interactions between organs, tissues, and cell types, mediated by cytokines or direct communication through cellular exchanges. Indeed, over the past decades, many peptides produced by adipose tissue, skeletal muscle and bone named adipokines, myokines and osteokines respectively, have been identified in mammals playing key roles in organ/tissue development and function. Some of them are released into the circulation acting as classical hormones, but they can also act locally showing autocrine/paracrine effects. In recent years, some of these cytokines have been identified in fish models of biomedical or agronomic interest. In this review, we will present their state of the art focusing on local actions and inter-tissue effects. Adipokines reported in fish adipocytes include adiponectin and leptin among others. We will focus on their structure characteristics, gene expression, receptors, and effects, in the adipose tissue itself, mainly regulating cell differentiation and metabolism, but in muscle and bone as target tissues too. Moreover, lipid metabolites, named lipokines, can also act as signaling molecules regulating metabolic homeostasis. Regarding myokines, the best documented in fish are myostatin and the insulin-like growth factors. This review summarizes their characteristics at a molecular level, and describes both, autocrine effects and interactions with adipose tissue and bone. Nonetheless, our understanding of the functions and mechanisms of action of many of these cytokines is still largely incomplete in fish, especially concerning osteokines (i.e., osteocalcin), whose potential cross talking roles remain to be elucidated. Furthermore, by using selective breeding or genetic tools, the formation of a specific tissue can be altered, highlighting the consequences on other tissues, and allowing the identification of communication signals. The specific effects of identified cytokines validated through in vitro models or in vivo trials will be described. Moreover, future scientific fronts (i.e., exosomes) and tools (i.e., co-cultures, organoids) for a better understanding of inter-organ crosstalk in fish will also be presented. As a final consideration, further identification of molecules involved in inter-tissue communication will open new avenues of knowledge in the control of fish homeostasis, as well as possible strategies to be applied in aquaculture or biomedicine.

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Section: New Approaches To Specific Analysis Of Tissue Interactionsmentioning

confidence: 99%

Recent advances in the crosstalk between adipose, muscle and bone tissues in fish

et al. 2023

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“…However, the most important applications are in situations where it is not trivial to obtain the “pure” component, and therefore use of regular deconvolution is impossible and/or inappropriate. For instance, with solvatochromic probes such as LAURDAN and Nile Red, there is no possibility to reproduce the exact environment present in cell membranes or lipidic organelles [ 26 , 43 , 44 , 45 , 56 ]. Before highlighting this application, let us cover some important considerations needed for a proper spectral phasor transformation.…”

Section: Results From Biological Measurementsmentioning

confidence: 99%

“…The spectral phasor approach to hyperspectral imaging analysis is highlighted using a complex problem, where Nile Red (NR) polarity profiles from different cellular environments are required to be analyzed from a specific cell line (adipocyte) on an in vivo zebrafish [ 56 ]. First problem to be faced is the analysis of Nile Red in the different subcellular organelles and membranes.…”

Section: Results From Biological Measurementsmentioning

confidence: 99%

Linear Combination Properties of the Phasor Space in Fluorescence Imaging

Torrado

Malacrida

Ranjit

2022

Sensors

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The phasor approach to fluorescence lifetime imaging, and more recently hyperspectral fluorescence imaging, has increased the use of these techniques, and improved the ease and intuitiveness of the data analysis. The fit-free nature of the phasor plots increases the speed of the analysis and reduces the dimensionality, optimization of data handling and storage. The reciprocity principle between the real and imaginary space—where the phasor and the pixel that the phasor originated from are linked and can be converted from one another—has helped the expansion of this method. The phasor coordinates calculated from a pixel, where multiple fluorescent species are present, depends on the phasor positions of those components. The relative positions are governed by the linear combination properties of the phasor space. According to this principle, the phasor position of a pixel with multiple components lies inside the polygon whose vertices are occupied by the phasor positions of these individual components and the distance between the image phasor to any of the vertices is inversely proportional to the fractional intensity contribution of that component to the total fluorescence from that image pixel. The higher the fractional intensity contribution of a vertex, the closer is the resultant phasor. The linear additivity in the phasor space can be exploited to obtain the fractional intensity contribution from multiple species and quantify their contribution. This review details the various mathematical models that can be used to obtain two/three/four components from phasor space with known phasor signatures and then how to obtain both the fractional intensities and phasor positions without any prior knowledge of either, assuming they are mono-exponential in nature. We note that other than for blind components, there are no restrictions on the type of the decay or their phasor positions for linear combinations to be valid—and they are applicable to complicated fluorescence lifetime decays from components with intensity decays described by multi-exponentials.

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“… ABSTRACT First Person is a series of interviews with the first authors of a selection of papers published in Biology Open, helping early-career researchers promote themselves alongside their papers. Paola Lepanto and Florencia Levin-Ferreyra are co-first authors on ‘ Insights into in vivo adipocyte differentiation through cell-specific labeling in zebrafish ’, published in BiO. Paola is a research assistant in the lab of José Badano at Institut Pasteur Montevideo, investigating cellular and developmental biology.…”

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confidence: 99%

First person – Paola Lepanto and Florencia Levin-Ferreyra

2021

Biology Open

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First Person is a series of interviews with the first authors of a selection of papers published in Biology Open, helping early-career researchers promote themselves alongside their papers. Paola Lepanto and Florencia Levin-Ferreyra are co-first authors on ‘ Insights into in vivo adipocyte differentiation through cell-specific labeling in zebrafish’, published in BiO. Paola is a research assistant in the lab of José Badano at Institut Pasteur Montevideo, investigating cellular and developmental biology. Florencia conducted the research described in this article while a final dissertation student in José Badano's lab at Institut Pasteur Montevideo. She is now a research associate in the lab of Bruno di Stefano at Baylor College of Medicine, Houston, USA, investigating stem cell potency and cell fate decisions.

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Insights into in vivo adipocyte differentiation through cell-specific labeling in zebrafish

Cited by 4 publications

References 61 publications

Recent advances in the crosstalk between adipose, muscle and bone tissues in fish

Recent advances in the crosstalk between adipose, muscle and bone tissues in fish

Linear Combination Properties of the Phasor Space in Fluorescence Imaging

First person – Paola Lepanto and Florencia Levin-Ferreyra

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