Insights into In Vitro Wound Closure on Two Biopolyesters—Polylactide and Polyhydroxyoctanoate

Witko, Tomasz; Solarz, Daria; Feliksiak, Karolina; Haraźna, Katarzyna; Rajfur, Zenon; Guzik, Maciej

doi:10.3390/ma13122793

Cited by 11 publications

(18 citation statements)

References 57 publications

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“…P(3HO) is already known for its numerous advantages in terms of biocompatibility and biodegradability. Due to its beneficial elasticity (Young's modulus 11.4-34 MPa) [4,14], endurance (friction coefficient 0.198-0.202), and low fragility (hardness 3.2-4.5 MPa) [1], it is being reviewed in terms of potential heart valve replacement, in wound-healing studies, and for joint endoprostheses [15][16][17]. Although in our previous works we ran a series of experiments describing the influence of P(3HO) on cell behavior, migration, morphology, and cytoskeleton, still there are some uncovered areas of interest on the specific substrate-cell interaction [1,4,18].…”

Section: P(3ho)mentioning

confidence: 99%

Vimentin Cytoskeleton Architecture Analysis on Polylactide and Polyhydroxyoctanoate Substrates for Cell Culturing

Feliksiak

Solarz

Guzik

et al. 2021

IJMS

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Polylactide (PLA), widely used in bioengineering and medicine, gained popularity due to its biocompatibility and biodegradability. Natural origin and eco-friendly background encourage the search of novel materials with such features, such as polyhydroxyoctanoate (P(3HO)), a polyester of bacterial origin. Physicochemical features of both P(3HO) and PLA have an impact on cellular response 32, i.e., adhesion, migration, and cell morphology, based on the signaling and changes in the architecture of the three cytoskeletal networks: microfilaments (F-actin), microtubules, and intermediate filaments (IF). To investigate the role of IF in the cellular response to the substrate, we focused on vimentin intermediate filaments (VIFs), present in mouse embryonic fibroblast cells (MEF). VIFs maintain cell integrity and protect it from external mechanical stress, and also take part in the transmission of signals from the exterior of the cell to its inner organelles, which is under constant investigation. Physiochemical properties of a substrate have an impact on cells’ morphology, and thus on cytoskeleton network signaling and assembly. In this work, we show how PLA and P(3HO) crystallinity and hydrophilicity influence VIFs, and we identify that two different types of vimentin cytoskeleton architecture: network “classic” and “nutshell-like” are expressed by MEFs in different numbers of cells depending on substrate features.

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Section: P(3ho)mentioning

confidence: 99%

Vimentin Cytoskeleton Architecture Analysis on Polylactide and Polyhydroxyoctanoate Substrates for Cell Culturing

Feliksiak

Solarz

Guzik

et al. 2021

IJMS

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“…The authors reported that the surface properties of P(3HO), i.e., the coefficient of friction, combined with lower brittleness of the materials compared to other biodegradable and biocompatible polyesters such as poly(lactic acid) (PLA) and P(3HB), may indicate potential applications of P(3HO) in the construction of medical implants, joint endoprostheses. It should be underlined that the above materials are not only subjected to significant loads (hip joint, knee joint), but also high friction forces due to their movement in contact with bones or cartilage [ 30 , 45 ].…”

Section: Discussionmentioning

confidence: 99%

“…Observations were made with an air 10× objective. For DAPI stain excitement a wave with length of 405 nm was applied [ 45 ].…”

Section: Methodsmentioning

confidence: 99%

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Physicochemical and Biological Characterisation of Diclofenac Oligomeric Poly(3-hydroxyoctanoate) Hybrids as β-TCP Ceramics Modifiers for Bone Tissue Regeneration

Haraźna

Cichoń

Skibiński

et al. 2020

IJMS

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Nowadays, regenerative medicine faces a major challenge in providing new, functional materials that will meet the characteristics desired to replenish and grow new tissue. Therefore, this study presents new ceramic-polymer composites in which the matrix consists of tricalcium phosphates covered with blends containing a chemically bounded diclofenac with the biocompatible polymer—poly(3-hydroxyoctanoate), P(3HO). Modification of P(3HO) oligomers was confirmed by NMR, IR and XPS. Moreover, obtained oligomers and their blends were subjected to an in-depth characterisation using GPC, TGA, DSC and AFM. Furthermore, we demonstrate that the hydrophobicity and surface free energy values of blends decreased with the amount of diclofenac modified oligomers. Subsequently, the designed composites were used as a substrate for growth of the pre-osteoblast cell line (MC3T3-E1). An in vitro biocompatibility study showed that the composite with the lowest concentration of the proposed drug is within the range assumed to be non-toxic (viability above 70%). Cell proliferation was visualised using the SEM method, whereas the observation of cell penetration into the scaffold was carried out by confocal microscopy. Thus, it can be an ideal new functional bone tissue substitute, allowing not only the regeneration and restoration of the defect but also inhibiting the development of chronic inflammation.

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“…Since all these reports bring up other ideas in the final establishment of the processes standing behind the nucleus mechanics, we decided to check the influence of the microenvironment on the presence of the nuclei invaginations and blebs. To explore this further, we stained vimentin, microtubules, actin, and nucleus, and used confocal microscopy imaging for mouse embryonic fibroblast 3T3 cells, cultured on glass, and two substrates of high potential use in medicine: polylactide (PLA) [ 25 , 26 ] and polyhydroxyoctanoate (PHO)—a polymer of biological origin [ 27 , 28 ].…”

Section: Introductionmentioning

confidence: 99%

Vimentin Association with Nuclear Grooves in Normal MEF 3T3 Cells

Feliksiak

Witko

Solarz

et al. 2020

IJMS

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Vimentin, an intermediate filament protein present in leukocytes, blood vessel endothelial cells, and multiple mesenchymal cells, such as mouse embryonic fibroblasts (MEF 3T3), is crucial for various cellular processes, as well as for maintaining the integrity and durability (stability) of the cell cytoskeleton. Vimentin intermediate filaments (VIFs) adhere tightly to the nucleus and spread to the lamellipodium and tail of the cell, serving as a connector between the nucleus, and the cell’s edges, especially in terms of transferring mechanical signals throughout the cell. How these signals are transmitted exactly remains under investigation. In the presented work, we propose that vimentin is involved in that transition by influencing the shape of the nucleus through the formation of nuclear blebs and grooves, as demonstrated by microscopic observations of healthy MEF (3T3) cells. Grooved, or “coffee beans” nuclei, have, to date, been noticed in several healthy cells; however, these structures are especially frequent in cancer cells—they serve as a significant marker for recognition of multiple cancers. We observed 288 MEF3T3 cells cultured on polyhydroxyoctanoate (PHO), polylactide (PLA), and glass, and we identified grooves, coaligned with vimentin fibers in the nuclei of 47% of cells cultured on PHO, 50% of cells on glass, and 59% of cells growing on PLA. We also observed nuclear blebs and associated their occurrence with the type of substrate used for cell culture. We propose that the higher rate of blebs in the nuclei of cells, cultured on PLA, is related to the microenvironmental features of the substrate, pH in particular.

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Insights into In Vitro Wound Closure on Two Biopolyesters—Polylactide and Polyhydroxyoctanoate

Cited by 11 publications

References 57 publications

Vimentin Cytoskeleton Architecture Analysis on Polylactide and Polyhydroxyoctanoate Substrates for Cell Culturing

Vimentin Cytoskeleton Architecture Analysis on Polylactide and Polyhydroxyoctanoate Substrates for Cell Culturing

Physicochemical and Biological Characterisation of Diclofenac Oligomeric Poly(3-hydroxyoctanoate) Hybrids as β-TCP Ceramics Modifiers for Bone Tissue Regeneration

Vimentin Association with Nuclear Grooves in Normal MEF 3T3 Cells

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