Insights into Mechanism of Aβ₄₂ Fibril Growth on Surface of Graphene Oxides: Oxidative Degree Matters

Abstract

The filamentous β‐amyloid deposition has been regarded as the hallmark pathology of Alzheimer's disease (AD). Nanomaterials such as graphene oxides (GOs) have achieved significant progress in the therapy of AD, but the molecular pathway of the growth propagation remains challenging to investigate, especially on the surfaces of materials. The thermodynamics and kinetics of fibril elongation on GO surfaces with different oxidative degrees have been investigated by a combination of in vitro experiments and simulations. ThT kinetics, calorimetric measurements, and TEM observations suggest that low oxidative GO‐10 promotes the fibril elongation, while both high oxidative GO‐20 and GO‐40 inhibit the fibril elongation. Computational results reveal that the apparent regulation behaviors of GOs on filament growth depend on the balance between the promoting effect by templating the incoming of monomers and the retarding effect by capturing the monomer during docking and locking phases through hydrogen bonding. This work will promote the understanding of the interplay between biomolecules and materials, thus providing new thoughts for the rational design of novel materials for amyloidosis therapy.

Encountering and Wrestling: Neutrophils Recognize and Defensively Degrade Graphene Oxide

“…Sustainable nanotechnology requires a comprehensive understanding of the interactions between immune system and material including exposure pathways and fate in healthcare. [8,50] Our study of the interaction between immune cells and nanomaterials elucidated the immune effect. Our research provides fundamental guidance and experimental evidences for practical application of GO in the nanotechnology and unintended exposures.…”

Activable Targeted Protein Degradation Platform Based on Light-triggered Singlet Oxygen

“…Circular dichroism (CD) spectroscopy is a simple and accurate method to determine the secondary structure of a protein in solution. As shown in Figure G, ERα exhibited typical peaks at 208 and 222 nm, representing the presence of α-helix . ERα was incubated with PSDalpha at 37 °C for 3 h and then irradiated for 15 and 30 min.…”

“…As shown in Figure 3G, ERα exhibited typical peaks at 208 and 222 nm, representing the presence of α-helix. 37 ERα was incubated with PSDalpha at 37 °C for 3 h and then irradiated for 15 and 30 min. The typical peaks of αhelix decreased significantly after 15 min irradiation, indicating that the structure of ERα was ruined by the generated 1 O 2 .…”

Targeted Recruitment and Degradation of Estrogen Receptor α by Photothermal Polydopamine Nanoparticles for Breast Tumor Ablation

“…CD spectroscopy is a fast, simple, and accurate method to investigate the conformation of proteins. [ 27 ] As ER α mainly composed of α ‐helix, their CD spectra exhibited negative peaks in the far UV region (Figure 2D). Time dependent degradation of ER α at different solvent temperature indicated that pure ER α in PBS degraded at 50–55 °C (Figure S12, Supporting Information).…”