Insights into Musa balbisiana and Musa acuminata species divergence and development of genic microsatellites by transcriptomics approach

Venkataramana, Ravishankar Kundapura; Sampangi-Ramaiah, Megha Hastantram; Rekha, A.; Khadke, G.N.; Chellam, Veerraju

doi:10.1016/j.plgene.2015.09.007

Cited by 30 publications

(15 citation statements)

References 29 publications

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“…In addition, the small number SSR markers out of thousands SSRs found in the genes of Indonesian local banana genotypes in this study could facilitate for other genetic studies. This results is relevant with the previous study by Venkataramana, Sampangi-Ramaiah, Ajitha, Khadke, & Chellam (2015) who explored approximately 4000 genic SSRs in M. balbisiana and M. acuminata which also proved the high genetic stability of in vitro regenerants. Floral axis is considered as a meristem tissue which actively divided and less contact to the ground (Resmi & Nair, 2011), therefore could eliminate the browning and budding propagation of banana, as demonstrated in this study.…”

Section: Assessment Of Genetic Stability In Acclimated Banana Plantssupporting

confidence: 83%

“…Similar results of SSR marker development from genome sequences were investigated by several research groups (D'Hont et al, 2012;Hippolyte et al, 2010;Ravishankar et al, 2012;Venkataramana, Sampangi-Ramaiah, Ajitha, Khadke, & Chellam, 2015). SSR markers were considered suitable for assessing genetic stability and true to type of a number of micropropagated plants in many crop species, such as potato, Dianthus giganteus subsp.…”

Section: Profile Of Newly Developed Ssr Markersmentioning

confidence: 75%

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Genetic Stability of Banana Plant Regenerated from Floral Axis Organogenesis Assessed by Newly Developed SSR Markers

et al. 2019

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Molecular marker is robust to precisely monitor the genetic stability of in vitro-banana plants. This study examined the genetic stability of 8 monthold banana plants of Soka variety derived from floral axis organogenesis using newly developed SSR markers. The results showed that the same qualitative and similar quantitative morphological characters of pseudostem, leaf and fruit were identified between mother plants and culture plants from floral axis regeneration. Both plants types were quite similar in number of tillers, brix percentage, fruit peel/mesocarp thickness and fruit length. Eleven out of 211 good quality of SSR loci showing high homology with important genes were selected for suitable PCR primers and produced unambiguous bands.The number of total bands was 323 for total SSR primers, in range of 20-60 per primer for total individual plants. Most culture plants showed identical with their mother plants, with very minor variation as reflected by genetic similarity coefficient range of 0.9-1.0. A high similar pattern on SSR to support morphological characters of mother plants and culture plants indicated a successful micropropagation using floral axis to encounter off-type clones.The floral axis organogenesis in this study is able to provide sufficient genetic materials of Soka for varietal registration and other applications.

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Section: Assessment Of Genetic Stability In Acclimated Banana Plantssupporting

confidence: 83%

Section: Profile Of Newly Developed Ssr Markersmentioning

confidence: 75%

Genetic Stability of Banana Plant Regenerated from Floral Axis Organogenesis Assessed by Newly Developed SSR Markers

et al. 2019

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“…In recent years many large‐scale efforts have sought to further understand these crops using genome sequences (Xu et al , ; D'Hont et al , ; Wang et al , ; Tamiru et al , ; Yang et al , ; Li et al , ) and genome diversity studies (Bredeson et al , ; Hardigan et al , ; Nyine et al , ; Christelová et al , ; Muñoz‐Rodríguez et al , ; Němečková et al , ), genetic selection (Wolfe et al , ), molecular markers (QTLs) (Monden and Tahara, ; Kim et al , ; Sharma and Bryan, ), and comparative transcriptome resources (Kundapura Venkataramana et al , ; Sarah et al , ; van Wesemael et al , ; Cenci et al , ) widely developed alongside morphologic, agronomic and phenotypic classifications (Oliveira et al , ; Rahajeng and Rahayuningsih, ; Dépigny et al , ; Girma et al , ; van Wesemael et al , ). The progress of the CGIAR Research Program on Roots, Tubers and Bananas (http://www.rtb.cgiar.org), applying genomics‐assisted breeding to RTBs, has recently been reviewed (Friedmann et al , ).…”

Section: Introductionmentioning

confidence: 99%

Metabolite database for root, tuber, and banana crops to facilitate modern breeding in understudied crops

et al. 2020

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Summary Roots, tubers, and bananas (RTB) are vital staples for food security in the world's poorest nations. A major constraint to current RTB breeding programmes is limited knowledge on the available diversity due to lack of efficient germplasm characterization and structure. In recent years large‐scale efforts have begun to elucidate the genetic and phenotypic diversity of germplasm collections and populations and, yet, biochemical measurements have often been overlooked despite metabolite composition being directly associated with agronomic and consumer traits. Here we present a compound database and concentration range for metabolites detected in the major RTB crops: banana (Musa spp.), cassava (Manihot esculenta), potato (Solanum tuberosum), sweet potato (Ipomoea batatas), and yam (Dioscorea spp.), following metabolomics‐based diversity screening of global collections held within the CGIAR institutes. The dataset including 711 chemical features provides a valuable resource regarding the comparative biochemical composition of each RTB crop and highlights the potential diversity available for incorporation into crop improvement programmes. Particularly, the tropical crops cassava, sweet potato and banana displayed more complex compositional metabolite profiles with representations of up to 22 chemical classes (unknowns excluded) than that of potato, for which only metabolites from 10 chemical classes were detected. Additionally, over 20% of biochemical signatures remained unidentified for every crop analyzed. Integration of metabolomics with the on‐going genomic and phenotypic studies will enhance ’omics‐wide associations of molecular signatures with agronomic and consumer traits via easily quantifiable biochemical markers to aid gene discovery and functional characterization.

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“…The degree of substitution was established by following the method proposed by Hong et al [28] A target composed of isolated starch was titrated. The degree of substitution (DS) was calculated by using Equation (2).…”

Section: Methodsmentioning

confidence: 99%

Thermal, Morphological, and Functional Characterization of Gros Michel Banana Starch Modified with Octenyl Succinic Anhydride

et al. 2020

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Banana is a food crop produced extensively in Colombia for fresh consumption. This fruit in the green state has a large amount of starch, which-unlike other Musaceae-has a small granule size that makes it attractive for use in the food industry. This work characterizes physicochemically, morphologically, structurally, and functionally isolated and modified starches from Gros Michel banana. This characterization uses techniques like SEM, XRD, FTIR, DSC, RVA, and HPLC. The results show a morphological change, given that the granules display superficial adherence of small masses. The IR spectra shows molecular vibrations in the modified starch at 1578 and 1745 cm −1 ; the X-ray diffraction patterns show alteration of the 3D starch structure, given that it changes in large proportion from a hexagonal to a monoclinic conformation after the modification. The maximum viscosities obtained via RVA change from 1380 cP for the isolated starch to 1247 cP for the modified starch. The amylopectin/amylose ratio is altered after the modification, going from 75/25 to 87/13. Modified starches are a viable alternative for use in the food industry as additives to stabilize emulsions that do not need high processing temperatures.

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Insights into Musa balbisiana and Musa acuminata species divergence and development of genic microsatellites by transcriptomics approach

Cited by 30 publications

References 29 publications

Genetic Stability of Banana Plant Regenerated from Floral Axis Organogenesis Assessed by Newly Developed SSR Markers

Genetic Stability of Banana Plant Regenerated from Floral Axis Organogenesis Assessed by Newly Developed SSR Markers

Metabolite database for root, tuber, and banana crops to facilitate modern breeding in understudied crops

Thermal, Morphological, and Functional Characterization of Gros Michel Banana Starch Modified with Octenyl Succinic Anhydride

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