Insights into predicting diabetic nephropathy using urinary biomarkers

Khan, Naseer Ullah; Lin, Jing; Liu, Xukun; Li, Haiying; Lü, Wei; Zhong, Zhuning; Zhang, Huajie; Waqas, Muhammad; Shen, Liming

doi:10.1016/j.bbapap.2020.140475

Cited by 63 publications

(42 citation statements)

References 177 publications

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“…The main clinical manifestations of diabetic nephropathy have been revealed to be proteinuria and persistent decline in renal function ( 24 ). In addition, the occurrence and development of diabetic nephropathy have been revealed to promote the development of cardiovascular disease ( 25 ). Therefore, the need to develop a new therapy to suppress the damage caused by diabetic nephropathy in patients is urgent.…”

Section: Discussionmentioning

confidence: 99%

Apelin‑13 alleviates diabetic nephropathy by enhancing nitric oxide production and suppressing kidney tissue fibrosis

et al. 2021

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Diabetes is a serious metabolic disease, and the kidney damage induced by diabetes also seriously affects the survival of patients. Apelin is a molecule that plays a crucial role in lipid metabolism, and recent studies have revealed that apelin-13, a subtype of apelin, plays an important role in regulating blood glucose levels. However, the role of apelin-13 in diabetic nephropathy remains unclear. In the present study, a rat model of diabetic nephropathy was constructed by the injection of streptozocin (STZ). During this process, these rats were injected with apelin-13. The blood glucose, urine protein and insulin levels were determined weekly. Next, the expression of angiotensin domain type 1 receptor-associated protein (APJ), endothelial nitric oxide synthase (eNOS), E-cadherin and α-smooth muscle actin (α-SMA) in the kidney tissues was determined with western blotting. Then, the endothelial cells of glomerular vessels were cultured with high glucose medium. These cells were treated with apelin-13 for 24 h. Finally, cell viability of these cells and the expression of APJ, eNOS, E-cadherin and α-SMA in these cells were determined with western blotting. As a result, treatment of apelin-13 induced the lower levels of blood glucose and urine protein.In addition, application of apelin-13 promoted the production of insulin and alleviated the insulin resistance. Treatment with apelin-13 promoted the expression of APJ, eNOS and E-cadherin while it suppressed the expression of α-SMA in kidney tissues of rats and endothelial cells of glomerular vessels. Furthermore, application of apelin-13 also promoted the cell viability of these cells. In conclusion, apelin-13 relieved diabetic nephropathy by promoting the production of nitric oxide (NO) and alleviating the fibrosis of kidney tissues.

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Section: Discussionmentioning

confidence: 99%

Apelin‑13 alleviates diabetic nephropathy by enhancing nitric oxide production and suppressing kidney tissue fibrosis

et al. 2021

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“…Additionally, urine samples were obtained for white blood (WBC) and red blood cell (RBC) counts, as well as protein and creatinine quantitation. Among urinary protein levels, in addition to the urinary microalbumin/creatinine ratio (ACR), we analyzed several sensitive indicators of early renal damage: N-acetylglucosaminidase (NAG), microglobulin and urinary transferrin (TRU) [ 17 ]. Proteinuria was measured after 24 h urine collection, and defined as protein excretion 150 mg. Also, urine samples were investigated using positive dipstick test with qualitative examination of levels of urinary occult blood and protein (− to +++).…”

Section: Methodsmentioning

confidence: 99%

Risk factors associated with renal crescentic formation in pediatric Henoch–Schönlein purpura nephritis: a retrospective cohort study

et al. 2020

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Background The long-term prognosis of Henoch-Schönlein purpura (HSP) depends on the severity of renal involvement, and crescent formation is considered an important risk factor for poor prognosis of Henoch-Schönlein purpura nephritis (HSPN). The objective of this study was to evaluate factors affecting crescent formation in children with HSPN. Methods Demographic factors, clinical characteristics, and laboratory data of children with HSPN with or without crescents were retrospectively analyzed. Univariate and multivariate logistic regression analyses were used to determine the risk factors of crescent formation in HSPN. Results A total of 191 children with HSPN were enrolled in the study. There were 107 (56%) males and 84 (44%) females, with a median age of 7 years (range: 2 years–15 years). International Study of Kidney Disease in Children (ISKDC) grading was used to divide subjects into two groups: those without glomerular crescent formation (ISKDC grades I–II, n = 146 cases) and those with glomerular crescent formation (ISKDC grades III–V, n = 45 cases). Logistic regression analysis showed that higher urinary white blood cell (WBC) count (OR = 3.300; 95% CI, 1.119–9.739; P = 0.0306) and higher urinary microalbumin/creatinine ratio (ACR) (OR = 25.053; 95% CI, 1.354–463.708; P = 0.0305) were independent risk factors for the formation of crescents in HSPN. The area under the receiver operating characteristic curve of urinary WBC and ACR were 0.753 and 0.698 respectively, with the Hosmer and Lemeshow goodness-of-fit test (P = 0.0669, P > 0.05). Conclusion These results suggest that higher urinary WBC count and ACR should be strictly monitored for children with HSPN. Adequate clinical intervention for these risk factors may limit or prevent renal crescent formation.

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“…Additionally, urine samples were obtained for white blood (WBC) and red blood cell (RBC) counts, as well as protein and creatinine quantitation. Among urinary protein levels, in addition to the urinary microalbumin/creatinine ratio (ACR), we analyzed several sensitive indicators of early renal damage: Nacetylglucosaminidase (NAG), microglobulin and urinary transferrin (TRU) [17]. Proteinuria was measured after 24 hours urine collection, and de ned as protein excretion 150 mg. Also, urine samples were investigated using positive dipstick test with qualitative examination of levels of urinary occult blood and protein (-to +++).…”

Section: Patientsmentioning

confidence: 99%

Risk factors associated with renal crescentic formation in pediatric Henoch–Schönlein purpura nephritis: a retrospective cohort study

Song

Guo

Sheng

et al. 2020

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Background: The long-term prognosis of Henoch-Schönlein purpura (HSP) depends on the severity of renal involvement, and crescent formation is considered an important risk factor for poor prognosis of Henoch-Schönlein purpura nephritis (HSPN). The objective of this study was to evaluate factors affecting crescent formation in children with HSPN.Methods: Demographic factors, clinical characteristics, and laboratory data of children with HSPN with or without crescents were retrospectively analyzed. Univariate and multivariate logistic regression analyses were used to determine the risk factors of crescent formation in HSPN.Results: A total of 191 children with HSPN were enrolled in the study. There were 107 (56%) males and 84 (44%) females, with a median age of 7 years (range: 2 years–15 years). International Study of Kidney Disease in Children (ISKDC) grading was used to divide subjects into two groups: those without glomerular crescent formation (ISKDC grades I–II, n = 146 cases) and those with glomerular crescent formation (ISKDC grades III–V, n = 45 cases). Logistic regression analysis showed that higher urinary white blood cell (WBC) count (OR = 3.300; 95% CI, 1.119–9.739; P = 0.0306) and higher urinary microalbumin/creatinine ratio (ACR) (OR = 25.053; 95% CI, 1.354–463.708; P = 0.0305) were independent risk factors for the formation of crescents in HSPN. The area under the receiver operating characteristic curve of urinary WBC and ACR were 0.753 and 0.698 respectively, with the Hosmer and Lemeshow goodness-of-fit test (P = 0.0669, P > 0.05).Conclusion: These results suggest that higher urinary WBC count and ACR should be strictly monitored for children with HSPN. Adequate clinical intervention for these risk factors may limit or prevent renal crescent formation.

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Insights into predicting diabetic nephropathy using urinary biomarkers

Cited by 63 publications

References 177 publications

Apelin‑13 alleviates diabetic nephropathy by enhancing nitric oxide production and suppressing kidney tissue fibrosis

Apelin‑13 alleviates diabetic nephropathy by enhancing nitric oxide production and suppressing kidney tissue fibrosis

Risk factors associated with renal crescentic formation in pediatric Henoch–Schönlein purpura nephritis: a retrospective cohort study

Risk factors associated with renal crescentic formation in pediatric Henoch–Schönlein purpura nephritis: a retrospective cohort study

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