This study was aimed at enhancing pothole detection by combining sigmoid calibration function and entropy thresholding segmentation on UAV multispectral imagery. UAV imagery was acquired via the flying of the DJI Matrice 600 (M600) UAV system, with the MicaSense RedEdge imaging sensor mounted on its fixed wing. An endmember spectral pixel denoting pothole feature was selected and used as the base from which spectral radiance patterns of a pothole were analyzed. A field survey was carried out to measure pothole diameters, which were used as the base on which the pothole area was determined. Entropy thresholding segmentation was employed to classify potholes. The sigmoid calibration function was used to reconfigure spectral radiance properties of the UAV spectral bands to pothole features. The descriptive statistics was computed to determine radiance threshold values to be used in demarcating potholes from the reconfigured or calibrated spectral bands. The performance of the sigmoid calibration function was evaluated by analyzing the area under curve (AUC) results generated using the Relative Operating Characteristic (ROC) technique. Spectral radiance pattern analysis of the pothole surface revealed high radiance values in the red channel and low radiance values in the near-infrared (NIR) channels of the spectrum. The sigmoid calibration function radiometrically reconfigured UAV spectral bands based on a total of 500 sampled pixels of pothole surface obtained from all the spectral channels. Upon successful calibration of UAV radiometric properties to pothole surface, the reconfigured mean radiance values for pothole surface were noted to be 0.868, 0.886, 0.944, 0.211 and 0.863 for blue, green, red, NIR and red edge, respectively. The area under curve (AUC) results revealed the r2 values of 0.53, 0.35, 0.71, 0.19 and 0.35 for blue, green, red, NIR and red edge spectral channels, respectively. Overestimation of pothole 1 by both original and calibrated spectral channels was noted and can be attributed to the presence of soils adjacent to the pothole. However, calibrated red channel estimated pothole 2 and pothole 3 accurately, with a slight area deviation from the measured potholes. The results of this study emphasize the significance of reconfiguring radiometric properties of the UAV imagery for improved recognition of potholes.