Insights into sensory hair cell regeneration from the zebrafish lateral line

Kniss, Jonathan; Jiang, Linjia; Piotrowski, Tatjana

doi:10.1016/j.gde.2016.05.012

Cited by 74 publications

(64 citation statements)

References 82 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Hair cells in neuromasts are readily killed by various treatments such as aminoglycosides antibiotics, e.g., neomycin [25], or copper sulfate [26]. Contrary to the hair cells in the mammalian cochlea, however, hair cells in neuromasts are quickly regenerated by other cell types present in the neuromasts [27]. In fact, neuromast hair cells are continuously replaced by new hair cells over the normal life of the fish [28].…”

Section: Resultsmentioning

confidence: 99%

Neurotoxicity of a Biopesticide Analog on Zebrafish Larvae at Nanomolar Concentrations

Nasri

Valverde

Roche

et al. 2016

IJMS

View full text Add to dashboard Cite

Despite the ever-increasing role of pesticides in modern agriculture, their deleterious effects are still underexplored. Here we examine the effect of A6, a pesticide derived from the naturally-occurring α-terthienyl, and structurally related to the endocrine disrupting pesticides anilinopyrimidines, on living zebrafish larvae. We show that both A6 and an anilinopyrimidine, cyprodinyl, decrease larval survival and affect central neurons at micromolar concentrations. Focusing on a superficial and easily observable sensory system, the lateral line system, we found that defects in axonal and sensory cell regeneration can be observed at much lower doses, in the nanomolar range. We also show that A6 accumulates preferentially in lateral line neurons and hair cells. We examined whether A6 affects the expression of putative target genes, and found that genes involved in apoptosis/cell proliferation are down-regulated, as well as genes reflecting estrogen receptor activation, consistent with previous reports that anilinopyrimidines act as endocrine disruptors. On the other hand, canonical targets of endocrine signaling are not affected, suggesting that the neurotoxic effect of A6 may be due to the binding of this compound to a recently identified, neuron-specific estrogen receptor.

show abstract

Section: Resultsmentioning

confidence: 99%

Neurotoxicity of a Biopesticide Analog on Zebrafish Larvae at Nanomolar Concentrations

Nasri

Valverde

Roche

et al. 2016

IJMS

View full text Add to dashboard Cite

show abstract

“…However, production occurs throughout life in the ears of numerous non-mammalian species [5,6]. The reasons that prevents hair cell regeneration in mammals have been studied for many years, and it has become clear that some deregulated cellular signals in mammalian ears should contribute to putting the brakes on regeneration.…”

Section: Discussionmentioning

confidence: 99%

“…The sensory hair cells are susceptible to damage, and their damage is often caused by noise exposure, viral or bacterial infections, aging, and ototoxic drugs [4]. In sharks, bony fish, amphibians, reptiles, and birds, hair cell damage stimulates the surrounding supporting cells to acquire the hair cell phenotype, thus leading to spontaneous regeneration of hair cells [5]. However, the loss of hair cells is permanent in mature mammals.…”

Section: Yuchen Liu and Guohui Niementioning

confidence: 99%

Characterization of the Transcriptome of Hair Cell Regeneration in the Neonatal Mouse Utricle

Han

et al. 2018

Cell Physiol Biochem

View full text Add to dashboard Cite

Background/Aims: Hearing and balance deficits are mainly caused by loss of sensory inner ear hair cells. The key signals that control hair cell regeneration are of great interest. However, the molecular events by which the cellular signals mediate hair cell regeneration in the mouse utricle are largely unknown. Methods: In the present study, we investigated gene expression changes and related molecular pathways using RNA-seq and qRT-PCR in the newborn mouse utricle in response to neomycin-induced damage. Results: There were 302 and 624 genes that were found to be up-regulated and down-regulated in neomycin-treated samples. GO and KEGG pathway analyses of these genes revealed many deregulated cellular components, molecular functions, biological processes and signaling pathways that may be related to hair cell development. More importantly, the differentially expressed genes included 9 transcription factors from the zf-C2H2 family, and eight of them were consistently down-regulated during hair cell damage and subsequent regeneration. Conclusion: Our results provide a valuable source for future studies and highlighted some promising genes, pathways or processes that may be useful for therapeutic applications.

show abstract

“…These hair cells can regenerate, an ability that humans lack. In humans, damage or destruction of sensory hair cells in the inner ear can lead to debilitating hearing or balance deficits, especially in older adults (Kniss, Jiang, & Piotrowski, 2016). Larvae, adult zebrafish, and isolated hair cells can be used to study hearing loss in vertebrates.…”

Section: Multicellular Model Organismsmentioning

confidence: 99%

Microfluidics for mechanobiology of model organisms

Kim

Nekimken

Fechner

et al. 2018

Methods in Cell Biology

View full text Add to dashboard Cite

Mechanical stimuli play a critical role in organ development, tissue homeostasis, and disease. Understanding how mechanical signals are processed in multicellular model systems is critical for connecting cellular processes to tissue- and organism-level responses. However, progress in the field that studies these phenomena, mechanobiology, has been limited by lack of appropriate experimental techniques for applying repeatable mechanical stimuli to intact organs and model organisms. Microfluidic platforms, a subgroup of microsystems that use liquid flow for manipulation of objects, are a promising tool for studying mechanobiology of small model organisms due to their size scale and ease of customization. In this work, we describe design considerations involved in developing a microfluidic device for studying mechanobiology. Then, focusing on worms, fruit flies, and zebrafish, we review current microfluidic platforms for mechanobiology of multicellular model organisms and their tissues and highlight research opportunities in this developing field.

show abstract

Insights into sensory hair cell regeneration from the zebrafish lateral line

Cited by 74 publications

References 82 publications

Neurotoxicity of a Biopesticide Analog on Zebrafish Larvae at Nanomolar Concentrations

Neurotoxicity of a Biopesticide Analog on Zebrafish Larvae at Nanomolar Concentrations

Characterization of the Transcriptome of Hair Cell Regeneration in the Neonatal Mouse Utricle

Microfluidics for mechanobiology of model organisms

Contact Info

Product

Resources

About