Insights into sucrose pathway of chicory stems by integrative transcriptomic and metabolic analyses

Testone, Giulio; Sobolev, Anatoly P.; Gonnella, Maria; Renna, Massimiliano; Mannina, Luisa; Capitani, Donatella; Arnesi, Giuseppe; Biancari, Tiziano; Giannino, Donato

doi:10.1016/j.phytochem.2019.112086

Cited by 2 publications

(1 citation statement)

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“…For chicory breeding efforts targeting laticifers or the production of metabolites in the laticifers, it is therefore essential to acquire basic information about the metabolite and transcriptome pattern of different plant tissues across developmental stages. However, chicory is not a model species and there are few transcriptomic, metabolomic and genomic data available until now (Testone et al , 2017, Testone et al , 2019, Peña-Espinoza et al , 2020). Therefore, in this study we set out to determine the metabolite and transcription profiles of several tissues of chicory roots, including latex, which is the cytosolic compartment of the laticifers.…”

Section: Introductionmentioning

confidence: 99%

Metabolome and transcriptome profiling of root chicory provide insights into laticifer development and specialized metabolism

Vahabi,

Balcke,

Hakkert

et al. 2024

Preprint

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Chicory roots produce inulin, a dietary fiber, as well as large quantities of bitter sesquiterpene lactones (STLs), which have valuable biological activities. In an effort to understand the compartmentalization of metabolism within chicory roots and the molecular basis of the development of laticifers that produce the chicory latex, we performed metabolomics and transcriptomics profiling. GC-MS and LC-MS identified a total of 22 580 features of which 135 were differentially abundant between cell types. Further analysis indicated that the major STLs accumulated primarily in the latex. Gene expression of known STL pathway genes indicates a compartmentalization of the biosynthesis across multiple tissues, with implications regarding the trafficking of pathway intermediates. Phytohormone measurements and gene expression analysis point to a major role for jasmonate signaling in the development and differentiation of laticifers. Furthermore, inulin accumulates mostly outside the laticifers but expression of inulin metabolic genes also point to a complex distribution and trafficking of inulin or inulin precursors across different root compartments. Altogether, the data presented here constitute a unique resource to investigate several biological processes in chicory roots, including laticifer development, STL biosynthesis and transport and inulin biosynthesis regulation.Significance statementA combination of transcriptomics, targeted and untargeted metabolomics of different tissues of chicory roots was generated. These data constitute a resource basis for the investigation of various processes taking place in chicory taproots, including sesquiterpene lactone biosynthesis, laticifer development and inulin biosynthesis and trafficking.

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Section: Introductionmentioning

confidence: 99%

Metabolome and transcriptome profiling of root chicory provide insights into laticifer development and specialized metabolism

Vahabi,

Balcke,

Hakkert

et al. 2024

Preprint

View full text Add to dashboard Cite

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Sugar Metabolism in Climacteric and Non‐Climacteric Melon

Stroka¹,

Schemberger²,

Ayub³

2021

Annual Plant Reviews Online

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Sugar content is one of the most important quality attributes of melon fruits. Understanding the molecular mechanisms involved in the process of sucrose accumulation is essential for possible genetic improvements and optimal fruit cultivation, especially with regard to differences intrinsic to climacteric and non‐climacteric fruits. The climacteric physiology and biochemistry are better understood, while with non‐climacteric fruits many gaps remain. This article focuses on the metabolic pathway of the Raffinose family oligosaccharides in melon fruits from different maturation groups, with an emphasis on potential candidates for manipulation, such as the different genes of sucrose synthase, invertase inhibitors, genes involved in sucrose hydrolysis‐resynthesis, and of the trehalose pathway, recently reported. Such insights may contribute to the understanding of the sucrose accumulation process and its variety‐dependent factors and point new directions for the development of technologies that will increase the quality of the melon fruits and of the entire Cucurbitaceae family.

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Insights into sucrose pathway of chicory stems by integrative transcriptomic and metabolic analyses

Cited by 2 publications

References 56 publications

Metabolome and transcriptome profiling of root chicory provide insights into laticifer development and specialized metabolism

Metabolome and transcriptome profiling of root chicory provide insights into laticifer development and specialized metabolism

Sugar Metabolism in Climacteric and Non‐Climacteric Melon

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