Insights into the Cardiotoxic Effects of Veratrum Lobelianum Alkaloids: Pilot Study

Taldaev, Amir; Terekhov, Roman; Melnik, E. V.; Белова, М. В.; Kozin, Sergey V.; Nedorubov, Andrey; Pomerantseva, Tatyana Ya; Ramenskaya, G. V.

doi:10.3390/toxins14070490

Cited by 5 publications

(4 citation statements)

References 48 publications

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“…Veratrum lobelianum is a robust herbaceous plant found in Northeast Asia, Central Europe, and North America that is known for its toxicity in humans and animals [33]. The alkaloid jervine, abundant in V. lobelianum, exhibits antitumor effects and influences DNA damage repair in nasopharyngeal carcinoma cells [24,27,34]. Our experiments, using jervine-containing V. lobelianum extract, activated the DNA damage checkpoint by downregulating hedgehog signaling, suggesting a complex relationship (Figures 3 and 4).…”

Section: Lobelianummentioning

confidence: 72%

From Hemp Waste to Bioactive Nanofiber Composites: Deep Eutectic Solvents and Electrospinning in Upcycling Endeavors

Mouro,

Gomes,

Gouveia

2023

Preprint

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Natural fibers have attracted increasing interest as an alternative to produce environmentally friendly and sustainable materials. Particularly, hemp fibers have revealed to be widely used in various industrial applications due to their extremely unique properties. However, hemp can generate a large amount of agro-waste, and results in an attractive source of biopolymers for the development of low-cost materials in alternative to the raw materials and conventional petroleum-based plastics. In addition, deep eutectic solvents (DESs), a new type of truly green solvents, have been tested to remove gums, lignin, and other non-cellulosic components from hemp fibers. Reusing these components dissolved into the DESs to fabricate new materials directly by electrospinning is a very attractive but still unexplored endeavor. Thus, this innovative research to venture new upcycling pathways is focused on the fabrication of composite nanofibers by electrospinning of a gel-based blend of Poly(vinyl alcohol) (PVA) and hemp agro-waste (HW) dissolved into choline chloride (ChCl) : Glycerol (1:2) and ChCl : Urea (1:2) DES mixtures. The results obtained revealed that the produced nanofibers displayed uniform appearance with diameters ranging from 257.72 ± 65.63 nm to 380.78 ± 133.98 nm. In addition, the mechanical properties of the electro-spun composite nanofibers produced from the gel-based blends of HW dissolved in DESs and PVA (HW-DESs_PVA) were found to be superior, resulting in an enhanced tensile strength and Young’s modulus. Furthermore, the incorporation of HW into the nanofibers was able to provide bioactive antioxidant and antibacterial properties. Notably, Onobrychis cornuta and Veratrum lobelianum extracts activated a DNA damage checkpoint response via the ATM/ATR and CHK-1 pathways; hindering germline development. Our LC‒MS analysis revealed jervine in V. lobelianum and nine antitumor compounds in O. cornuta. Interestingly, linoleic acid replicated phenotypes induced by O. cornuta exposure, including an increased level of pCHK-1 foci, apoptosis, and the MAPK pathway. We observed that mutants in the MAPK pathway suppressed the reduction in worm survival, emphasizing its role in survivability, meiotic progression, and DNA damage checkpoint-mediated apoptosis. This study reveals complex interactions between herbal extracts and C. elegans processes, shedding light on potential antitumor effects and mechanisms. The findings provide insights into the complex landscape of herbal medicine's impact on a model organism, offering implications for broader applications.

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Section: Lobelianummentioning

confidence: 72%

From Hemp Waste to Bioactive Nanofiber Composites: Deep Eutectic Solvents and Electrospinning in Upcycling Endeavors

Mouro,

Gomes,

Gouveia

2023

Preprint

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“…We hypothesized that the compounds present in the herbs may contribute to the observed phenotypes in the herbal extracts. Jervine, a primary alkaloid found in V. lobelianum [ 23 , 24 ], has been identified as an inhibitor of the Hedgehog pathway in nasopharyngeal carcinoma—a crucial cellular pathway involved in cell growth, differentiation, and tissue formation [ 25 , 26 , 27 ].…”

Section: Resultsmentioning

confidence: 99%

“…Veratrum lobelianum is a robust herbaceous plant found in Northeast Asia, Central Europe, and North America that is known for its toxicity in humans and animals [ 33 ]. The alkaloid jervine, abundant in V. lobelianum , exhibits antitumor effects and influences DNA damage repair in nasopharyngeal carcinoma cells [ 24 , 27 , 34 ]. Our experiments, using jervine-containing V. lobelianum extract, activated the DNA damage checkpoint by downregulating hedgehog signaling, suggesting a complex relationship ( Figure 3 and Figure 4 ).…”

Section: Discussionmentioning

confidence: 99%

Exploring the Impact of Onobrychis cornuta and Veratrum lobelianum Extracts on C. elegans: Implications for MAPK Modulation, Germline Development, and Antitumor Properties

Meng,

Pathak,

Ren

et al. 2023

Nutrients

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In an era of increasing interest in the potential health benefits of medicinal foods, the need to assess their safety and potential toxicity remains a critical concern. While these natural remedies have garnered substantial attention for their therapeutic potential, a comprehensive understanding of their effects on living organisms is essential. We examined 316 herbal extracts to determine their potential nematocidal attributes in Caenorhabditis elegans. Approximately 16% of these extracts exhibited the capacity to induce diminished survival rates and larval arrest, establishing a correlation between larval arrest and overall worm viability. Certain extracts led to an unexpected increase in male nematodes, accompanied by a discernible reduction in DAPI-stained bivalent structures and perturbed meiotic advancement, thereby disrupting the conventional developmental processes. Notably, Onobrychis cornuta and Veratrum lobelianum extracts activated a DNA damage checkpoint response via the ATM/ATR and CHK-1 pathways, thus hindering germline development. Our LC–MS analysis revealed jervine in V. lobelianum and nine antitumor compounds in O. cornuta. Interestingly, linoleic acid replicated phenotypes induced by O. cornuta exposure, including an increased level of pCHK-1 foci, apoptosis, and the MAPK pathway. Mutants in the MAPK pathway mitigated the decline in worm survival, underscoring its importance in promoting worm viability. This study reveals complex interactions between herbal extracts and C. elegans processes, shedding light on potential antitumor effects and mechanisms. The findings provide insights into the complex landscape of herbal medicine’s impact on a model organism, offering implications for broader applications.

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“…According to the literature, gervin has anti-tumor [41,42], anti-inflammatory, analgesic [43], and radioprotective [44] activities. There is a study investigating the cardiotoxic mechanism of gervin, protoveratrin A, and B [45].…”

Section: Veratrum Speciesmentioning

confidence: 99%

IPPRAS Cryobank for the Conservation of Orthodox Seeds of Rare, Endangered, Medicinal, and Ornamental Plant Species—Current Research

Nits,

Sementsova,

Osipova

et al. 2024

Plants

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Plant cryobanks play a significant role in modern science and breeding. They contribute to the recovery of lost species, the emergence of new plant varieties, and help preserve and explore the diversity of the plant world. The IPPRAS Cryobank collection is constantly supplemented with new samples, while, at the same time, the stored samples are being monitored. In order to test seed germination, seeds of Allium and Veratrum species were thawed. Rare Allium species seeds, such as A. nutans, A. schoenoprasum, and A. victorialis were stored in liquid nitrogen for 17, 19, and 30 years, respectively. Long-term cryopreservation decreased germination rates for A. nutans from 96.55 to 50.00%, for A. schoenoprasum from 72.00 to 62.75%, and for A. victorialis from 90.00 to 83.05%. Seeds of a rare medicinal species, Veratrum lobelianum, were stored in liquid nitrogen for 18 years; the seed germination rate during this storage period has been significantly decreased from 75.00 to 14.81%. V. nigrum seeds were also collected and frozen in liquid nitrogen for 3 days. Short-term cryopreservation did not result in a statistically significant change in germination rates (from 79.71 to 82.69%). The seeds of an endangered ornamental species, Cypripedium calceolus, were collected and kept frozen for 3 days. After cryopreservation, the seeds were planted on three different media, as follows: ½ MS, MS with 10% coconut milk, and BM1. On ½ MS medium, 24.98% seeds formed protocorms, while on MS medium with 10% coconut milk, this number was 10.02%, and on BM1 medium, it was 15.02%, respectively; however, after 2.5 months, all of the protocorms died. Thus, it appears that the existing protocol for seed cryopreservation of C. calceolus needs further improvement. The size, weight, and free water content (WC) of six previously cryopreserved Stipa species and three Allium species were measured. For all the Allium and Stipa species studied, we found no correlation between seed size, WC, and cryotolerance. We also found no correlation between the life form, which reflects the water requirement of the species, and cryotolerance.

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Insights into the Cardiotoxic Effects of Veratrum Lobelianum Alkaloids: Pilot Study

Cited by 5 publications

References 48 publications

From Hemp Waste to Bioactive Nanofiber Composites: Deep Eutectic Solvents and Electrospinning in Upcycling Endeavors

From Hemp Waste to Bioactive Nanofiber Composites: Deep Eutectic Solvents and Electrospinning in Upcycling Endeavors

Exploring the Impact of Onobrychis cornuta and Veratrum lobelianum Extracts on C. elegans: Implications for MAPK Modulation, Germline Development, and Antitumor Properties

IPPRAS Cryobank for the Conservation of Orthodox Seeds of Rare, Endangered, Medicinal, and Ornamental Plant Species—Current Research

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