Insights into the impact of flhF inactivation on Campylobacter jejuni colonization of chick and mice gut

Ren, Fangzhe; Li, Xiaofei; Tang, Haiyan; Jiang, Qidong; Xi, Yun; Fang, Lin; Huang, Pingyu; Tang, Yuanyue; Li, Qiuchun; Huang, Jinlin; Jiao, Xinan

doi:10.1186/s12866-018-1318-1

Cited by 12 publications

(6 citation statements)

References 54 publications

(85 reference statements)

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“… 133 In addition to their function in bacterial motility, C. jejuni utilizes flagella for protein secretion, biofilm formation, and adhesion. 134 , 135 Flagellar motility of C. jejuni is associated with a chemotaxis system, which is essential for effective colonization in the avian and mammalian guts. C. jejuni displays a chemotactic response to amino acids and organic acids originating from either the host or the residual gut microbiota, in addition to distinct constituents of bile and mucus.…”

Section: Mouse Models For Enteropathogen Infectionmentioning

confidence: 99%

Mouse models for bacterial enteropathogen infections: insights into the role of colonization resistance

et al. 2023

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Globally, enteropathogenic bacteria are a major cause of morbidity and mortality. 1-3 Campylobacter, Salmonella , Shiga-toxin-producing Escherichia coli , and Listeria are among the top five most commonly reported zoonotic pathogens in the European Union. 4 However, not all individuals naturally exposed to enteropathogens go on to develop disease. This protection is attributable to colonization resistance (CR) conferred by the gut microbiota, as well as an array of physical, chemical, and immunological barriers that limit infection. Despite their importance for human health, a detailed understanding of gastrointestinal barriers to infection is lacking, and further research is required to investigate the mechanisms that underpin inter-individual differences in resistance to gastrointestinal infection. Here, we discuss the current mouse models available to study infections by non-typhoidal Salmonella strains, Citrobacter rodentium (as a model for enteropathogenic and enterohemorrhagic E. coli), Listeria monocytogenes , and Campylobacter jejuni. Clostridioides difficile is included as another important cause of enteric disease in which resistance is dependent upon CR. We outline which parameters of human infection are recapitulated in these mouse models, including the impact of CR, disease pathology, disease progression, and mucosal immune response. This will showcase common virulence strategies, highlight mechanistic differences, and help researchers from microbiology, infectiology, microbiome research, and mucosal immunology to select the optimal mouse model.

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Section: Mouse Models For Enteropathogen Infectionmentioning

confidence: 99%

Mouse models for bacterial enteropathogen infections: insights into the role of colonization resistance

et al. 2023

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“…Despite these effects, knowledge relating to how infection with Campylobacter species results in these outcomes is limited. For example, because whole-genome analysis of C. jejuni isolates suggests the pathogen lacks many of the prototypical secretion systems and virulence factors often associated with pathogenesis, gastrointestinal disease is hypothesized to be due to dysregulated activation of the host immune response through a factor or process unique to C. jejuni (Ren et al, 2018). Since little work has been done to understand the host response to C. jejuni and its consequence on gastrointestinal health, our group previously examined and observed neutrophils are recruited to the colon of infected animals and that predominantly neutrophil-derived proteins are elevated in the feces of C. jejuni -infected patients.…”

Section: Discussionmentioning

confidence: 99%

Battle for the histones: a secreted bacterial sirtuin from Campylobacter jejuni activates neutrophils and induces inflammation during infection

Callahan

Hancock

Doster

et al. 2022

Preprint

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Histone modifications alter numerous cornerstone processes in eukaryotes, including metabolism, physiology, and immunity. Numerous bacterial pathogens can alter expression of host-derived sirtuins to deacetylate histones in order to promote infection, yet, a bacterial-derived sirtuin has yet to be investigated to deacetylate host histones. Using Campylobacter jejuni, the leading cause of bacterial-derived gastroenteritis, we found a secreted sirtuin, SliP, which binds to and deacetylates neutrophil histones. We found neutrophil activation and extrusion of neutrophil extracellular traps was SliP dependent, whereby sliP mutants are unable to activate neutrophils or promote NETosis. Leveraging the mouse model of campylobacteriosis, we further demonstrate the sliP mutant can efficiently infect IL-10-/- mice, but induction of proinflammatory cytokine production and gastrointestinal pathology is SliP-dependent. In conclusion, we investigate a unique bacterial effector which targets host histones and is responsible for the inflammatory response and tissue pathology observed during campylobacteriosis.HighlightsC. jejuni encodes a secreted effector, SliP, which functions as a canonical sirtuinSliP binds to and deacetylates neutrophil histone H3 during bacterial infectionC. jejuni-induced neutrophil activation and NETosis are SliP-dependentInflammation and tissue pathology during C. jejuni infection is SliP-dependent

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“…Several studies investigated host adaptation, especially from C. jejuni, as this species shows a well-defined lineage separation based on MLST data that distinguish the population into host-specialist and host-generalist clonal complexes (Sheppard et al 2014). Several colonization studies revealed that modification and differential transcription of motility genes in C. jejuni play a key role in adaptation and transmission (Hermans et al 2011;de Vries et al 2017;Ren et al 2018). These data were supported by in vitro experiments as well as by genomic data and by RNA sequencing.…”

Section: Source Attribution In Clinical and Agricultural Settingmentioning

confidence: 93%

Population Biology and Comparative Genomics of Campylobacter Species

Epping

Antão

Semmler

2021

Current Topics in Microbiology and Immunology

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The zoonotic pathogen Campylobacter is the leading cause for bacterial foodborne infections in humans. Campylobacters are most commonly transmitted via the consumption of undercooked poultry meat or raw milk products. The decreasing costs of whole genome sequencing enabled large genome-based analyses of the evolution and population structure of this pathogen, as well as the development of novel high-throughput molecular typing methods. Here, we review the evolutionary development and the population diversity of the two most clinically relevant Campylobacter species; C. jejuni and C. coli. The state-of-the-art phylogenetic studies showed clustering of C. jejuni lineages into host specialists and generalists with coexisting lifestyles in chicken and livestock-associated hosts, as well as the separation of C. coli isolates of riparian origin (waterfowl, water) from C. coli isolated from clinical and farm-related samples. We will give an overview of recombination between both species and the potential impact of horizontal gene transfer on host adaptation in Campylobacter. Additionally, this review briefly places the current knowledge of the population structure of other Campylobacter species such as C. lari, C. concisus and C. upsaliensis into perspective. We also provide an overview of how molecular typing methods such as multilocus sequence typing (MLST) and whole genome MLST have been used to detect and trace Campylobacter outbreaks along the food chain.

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Insights into the impact of flhF inactivation on Campylobacter jejuni colonization of chick and mice gut

Cited by 12 publications

References 54 publications

Mouse models for bacterial enteropathogen infections: insights into the role of colonization resistance

Mouse models for bacterial enteropathogen infections: insights into the role of colonization resistance

Battle for the histones: a secreted bacterial sirtuin from Campylobacter jejuni activates neutrophils and induces inflammation during infection

Population Biology and Comparative Genomics of Campylobacter Species

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