Insights into the Interaction of Dacarbazine and Human Serum Albumin from Electrochemical Probing

Abstract

The interaction between dacarbazine (DAC) and human serum albumin (HSA) was investigated under physiological conditions using electrochemical techniques, including cyclic voltammetry (CV), differential pulse voltammetry (DPV), and electrochemical impedance spectroscopy (EIS). The CV results demonstrated that the oxidation of DAC on a pyrolytic graphite electrode (PGE) surface was irreversible and controlled by an adsorption-diffusion process. The addition of HSA was found to decrease the peak potential of DAC without altering the electrochemical parameters, which is likely due to the formation of an electro-inactive complex between the drug and protein, as supported by DPV and EIS measurements. Using DPV, the binding constant and stoichiometry of the complex were calculated to be 2.16×104 mol-1 L and 1:1, respectively. The temperature effect revealed that DAC binds to HSA through hydrophobic forces. In addition, the PGE electrode was successfully used to determine DAC in from biological samples.

Electrochemical and Theoretical Investigations on the Binding of Anticancer Drug Olaparib to Human Serum Albumin

“…Cyclic voltammetric investigations.-CV is an effective method for the direct analysis of protein-drug interactions under physiological conditions. [28][29][30][31][32] In this context, the electrochemical behavior of the K 3 Fe(CN) 6 /K 4 Fe(CN) 6 redox system on the GCE in the absence and presence of HSA, OLA, and HSA-OLA was investigated at a scan rate of 50 mV s −1 by CV, as shown in Fig. 3.…”

“…[16][17][18][19][20] Various methodologies have been used to investigate protein-drug interactions, including spectrometry, 21 fluorescence, 22 calorimetry, 23 circular dichroism (CD), 24 molecular modeling, 25 Raman spectroscopy, 26 nuclear magnetic resonance (NMR), 27 and electrochemical methods. [28][29][30][31][32] The voltametric techniques in electrochemical studies are noteworthy techniques due to its speed, efficiency, and cost-effectiveness. It is suitable for investigating the binding interaction between OLA and HSA, as it allows for examination of the electrochemical transfer process of biological reactions with high sensitivity and a broad dynamic range.…”

Development of a molecularly imprinted polymer-based electrochemical sensor for the selective detection of nerve agent VX metabolite ethyl methylphosphonic acid in human plasma and urine samples

“…To achieve plasma and urinary EMPA recognition in aqueous solutions, creating MIPs is required to bind the specific target without non-specific binding selectively. A MIP-based electrochemical sensor has been developed in this study with voltammetric techniques due to their unique properties such as low cost, fast response time, environment friendliness, and easy usage [ 15 , 17 – 19 ]. Concerning this matter, we aimed to design a MIP-based electrochemical sensor to achieve sensitive, selective, and precise determination of EMPA in plasma and urine samples.…”