Insights into the Jasmonate Signaling in Basal Land Plant Revealed by the Multi-Omics Analysis of an Antarctic Moss Pohlia nutans Treated with OPDA

Liu, Shenghao; Li, Tingting; Zhang, Pengying; Zhao, Linlin; Zhang, Zhaohui; Cong, Bin

doi:10.3390/ijms232113507

Cited by 4 publications

(3 citation statements)

References 67 publications

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“…A standard procedure of widely targeted metabolomics technique was used to extract the samples, identify the metabolites, and quantify their contents [ 87 , 88 , 89 ]. The “widely targeted metabolomics technique” was developed based on multiple reaction monitoring (MRM), which is a new integrated technique for the large-scale detection, identification, and quantification of metabolites in a high throughput manner [ 62 , 90 ]. Principal component analysis (PCA), orthogonal projections to latent structure-discriminant analysis (OPLS-DA), and hierarchical clustering analysis (HCA) were performed using the R package ( , accessed on 12 May 2022) to examine the accumulation of metabolites caused by UV-B radiation.…”

Section: Methodsmentioning

confidence: 99%

Genome-Wide Analysis of Long Non-Coding RNAs Related to UV-B Radiation in the Antarctic Moss Pohlia nutans

Fang

Cong

Zhao

et al. 2023

IJMS

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Antarctic organisms are consistently suffering from multiple environmental pressures, especially the strong UV radiation caused by the loss of the ozone layer. The mosses and lichens dominate the vegetation of the Antarctic continent, which grow and propagate in these harsh environments. However, the molecular mechanisms and related regulatory networks of these Antarctic plants against UV-B radiation are largely unknown. Here, we used an integrated multi-omics approach to study the regulatory mechanism of long non-coding RNAs (lncRNAs) of an Antarctic moss (Pohlia nutans) in response to UV-B radiation. We identified a total of 5729 lncRNA sequences by transcriptome sequencing, including 1459 differentially expressed lncRNAs (DELs). Through functional annotation, we found that the target genes of DELs were significantly enriched in plant-pathogen interaction and the flavonoid synthesis pathway. In addition, a total of 451 metabolites were detected by metabonomic analysis, and 97 differentially change metabolites (DCMs) were found. Flavonoids account for 20% of the total significantly up-regulated metabolites. In addition, the comprehensive transcriptome and metabolome analyses revealed the co-expression pattern of DELs and DCMs of flavonoids. Our results provide insights into the regulatory network of lncRNA under UV-B radiation and the adaptation of Antarctic moss to the polar environments.

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Section: Methodsmentioning

confidence: 99%

Genome-Wide Analysis of Long Non-Coding RNAs Related to UV-B Radiation in the Antarctic Moss Pohlia nutans

Fang

Cong

Zhao

et al. 2023

IJMS

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“…On the other hand, a stimulatory effect of 12-OPDA on GAPDH expression has been proposed based on studies with Physcomitrella patens mutant lines lacking AOS activity and 12-OPDA-treated Pohlia nutans [ 128 , 129 ]. Assessment of differentially expressed genes due to 12-OPDA treatment in various organisms ( P. nutans , variegated Epipremnum aureum , and the algae Klebsormidium nitens ) shows upregulation of gene orthologues of A. thaliana GAPDH isoforms (GAPCp1, GAPC2), whereas GAPA2 and GAPB orthologues are downregulated [ 79 , 129 , 130 ]. The only direct study of 12-OPDA-responsive genes (ORGs) in A. thaliana , conducted by Taki et al, does not show significant regulation of GAPDH expression by 12-OPDA.…”

Section: Influence Of Oxylipins On the Redox-regulatory Networkmentioning

confidence: 99%

Oxylipins and Reactive Carbonyls as Regulators of the Plant Redox and Reactive Oxygen Species Network under Stress

2023

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Reactive oxygen species (ROS), and in particular H2O2, serve as essential second messengers at low concentrations. However, excessive ROS accumulation leads to severe and irreversible cell damage. Hence, control of ROS levels is needed, especially under non-optimal growth conditions caused by abiotic or biotic stresses, which at least initially stimulate ROS synthesis. A complex network of thiol-sensitive proteins is instrumental in realizing tight ROS control; this is called the redox regulatory network. It consists of sensors, input elements, transmitters, and targets. Recent evidence revealed that the interplay of the redox network and oxylipins–molecules derived from oxygenation of polyunsaturated fatty acids, especially under high ROS levels–plays a decisive role in coupling ROS generation and subsequent stress defense signaling pathways in plants. This review aims to provide a broad overview of the current knowledge on the interaction of distinct oxylipins generated enzymatically (12-OPDA, 4-HNE, phytoprostanes) or non-enzymatically (MDA, acrolein) and components of the redox network. Further, recent findings on the contribution of oxylipins to environmental acclimatization will be discussed using flooding, herbivory, and establishment of thermotolerance as prime examples of relevant biotic and abiotic stresses.

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“…These results illustrate that OPDA regulates the production of PSMs independent of JA-Ile signaling. Widely targeted metabolomics analyses of the OPDA-induced metabolome in Antarctic moss (Pohlia nutans) show that a total of 82 metabolites were significantly changed by OPDA treatment, including alkaloids, phenolic acids, flavonoids and amino acids and derivatives [79]. However, whether the induction of these PSMs by OPDA is independent of dn-OPDA, and if so, its underlying mechanism, awaits more comprehensive analyses.…”

Section: Secondary Metabolism Induced By Opda and Cjmentioning

confidence: 99%

Jasmonates Coordinate Secondary with Primary Metabolism

Luo,

Qiu,

Zhang

et al. 2023

Metabolites

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Jasmonates (JAs), including jasmonic acid (JA), its precursor 12-oxo-phytodienoic acid (OPDA) and its derivatives jasmonoyl-isoleucine (JA-Ile), methyl jasmonate (MeJA), cis-jasmone (CJ) and other oxylipins, are important in the regulation of a range of ecological interactions of plants with their abiotic and particularly their biotic environments. Plant secondary/specialized metabolites play critical roles in implementing these ecological functions of JAs. Pathway and transcriptional regulation analyses have established a central role of JA-Ile-mediated core signaling in promoting the biosynthesis of a great diversity of secondary metabolites. Here, we summarized the advances in JAs-induced secondary metabolites, particularly in secondary metabolites induced by OPDA and volatile organic compounds (VOCs) induced by CJ through signaling independent of JA-Ile. The roles of JAs in integrating and coordinating the primary and secondary metabolism, thereby orchestrating plant growth–defense tradeoffs, were highlighted and discussed. Finally, we provided perspectives on the improvement of the adaptability and resilience of plants to changing environments and the production of valuable phytochemicals by exploiting JAs-regulated secondary metabolites.

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Insights into the Jasmonate Signaling in Basal Land Plant Revealed by the Multi-Omics Analysis of an Antarctic Moss Pohlia nutans Treated with OPDA

Cited by 4 publications

References 67 publications

Genome-Wide Analysis of Long Non-Coding RNAs Related to UV-B Radiation in the Antarctic Moss Pohlia nutans

Genome-Wide Analysis of Long Non-Coding RNAs Related to UV-B Radiation in the Antarctic Moss Pohlia nutans

Oxylipins and Reactive Carbonyls as Regulators of the Plant Redox and Reactive Oxygen Species Network under Stress

Jasmonates Coordinate Secondary with Primary Metabolism

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