Insights into the Mechanisms of Heat Priming and Thermotolerance in Tobacco Pollen

Mareri, Lavinia; Faleri, Claudia; Aloisi, Iris; Parrotta, Luigi; Duca, Stefano Del; Cai, Giampiero

doi:10.3390/ijms22168535

Cited by 5 publications

(3 citation statements)

References 71 publications

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“…Another strategy is the application of priming, which consists of a gradual increase in temperature or a brief pre-exposure to a slightly supra-optimal but nonlethal temperature. Mareri et al [7] studied the effects of priming on pollen grain function in tobacco. The results showed that pre-exposure to sub-lethal temperatures (30 • C) positively improves pollen performance by altering its metabolism.…”

Section: Temperature Stressmentioning

confidence: 99%

Environmental Stress and Plants

Mareri

Parrotta

Cai

2022

IJMS

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Land plants are constantly subjected to multiple unfavorable or even adverse environmental conditions. Among them, abiotic stresses (such as salt, drought, heat, cold, heavy metals, ozone, UV radiation, and nutrient deficiencies) have detrimental effects on plant growth and productivity and are increasingly important considering the direct or indirect effects of climate change. Plants respond in many ways to abiotic stresses, from gene expression to physiology, from plant architecture to primary, and secondary metabolism. These complex changes allow plants to tolerate and/or adapt to adverse conditions. The complexity of plant response can be further influenced by the duration and intensity of stress, the plant genotype, the combination of different stresses, the exposed tissue and cell type, and the developmental stage at which plants perceive the stress. It is therefore important to understand more about how plants perceive stress conditions and how they respond and adapt (both in natural and anthropogenic environments). These concepts were the basis of the Special Issue that International Journal of Molecular Sciences expressly addressed to the relationship between environmental stresses and plants and that resulted in the publication of 5 reviews and 38 original research articles. The large participation of several authors and the good number of contributions testifies to the considerable interest that the topic currently receives in the plant science community, especially in the light of the foreseeable climate changes. Here, we briefly summarize the contributions included in the Special Issue, both original articles categorized by stress type and reviews that discuss more comprehensive responses to various stresses.

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Section: Temperature Stressmentioning

confidence: 99%

Environmental Stress and Plants

Mareri

Parrotta

Cai

2022

IJMS

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“…Plants, sedentary organisms, are at risk from these changes. The male gametophyte is extremely sensitive to temperature, and its ability to maintain its physiological state under heat stress is known as acquired thermo-tolerance 27 .…”

Section: Assisted Plant Migration: Potential Applicationmentioning

confidence: 99%

Collective excitations of germinating pollen grains at critical points

Pietruszka

2023

Sci Rep

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In plants, the germinating pollen grain (pollen tube) is a single, elongated cell that serves as a conduit through which gametes pass. Pollen tubes display a fast growth rate, which under certain conditions, changes periodically and is accompanied by ion exchange with the growth environment. Therefore, pollen tubes exposed to various abiotic conditions may adversely affect or improve their reproductive performance and fertility. We examined a collection of live pollen tubes of tobacco (Nicotiana tabacum L.) and hyacinth (Hyacinthus orientalis L.) using a non-invasive semiconductor–electrolyte interface technique in the vicinity of the germination temperature or optimum growth temperature of a pollen grains/tubes. The time series measurements and numerical calculations, performed using information theory methods, represent signatures of collective dynamics in living cells at critical—molecularly encoded—germination and growth temperatures. This method (and soil pH data) can facilitate assisted plant migrations from one ecosystem to another as the Earth faces climate change.

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“…An important adaptive trait for many land plants is an amazing ability to sense and adapt to changing temperatures (Lamers et al, 2020;Hayes et al, 2021;Mareri et al, 2021;Nishad and Nandi, 2021). Nevertheless, global climate change is predicted to make periods of heat stress increasingly detrimental to plant growth and reproduction (Challinor et al, 2014;Zhao et al, 2017;Cohen et al, 2021;Zandalinas et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

A Ratiometric Calcium Reporter CGf Reveals Calcium Dynamics Both in the Single Cell and Whole Plant Levels Under Heat Stress

et al. 2021

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Land plants evolved to quickly sense and adapt to temperature changes, such as hot days and cold nights. Given that calcium (Ca2+) signaling networks are implicated in most abiotic stress responses, heat-triggered changes in cytosolic Ca2+ were investigated in Arabidopsis leaves and pollen. Plants were engineered with a reporter called CGf, a ratiometric, genetically encoded Ca2+ reporter with an mCherry reference domain fused to an intensiometric Ca2+ reporter GCaMP6f. Relative changes in [Ca2+]cyt were estimated based on CGf’s apparent KD around 220 nM. The ratiometric output provided an opportunity to compare Ca2+ dynamics between different tissues, cell types, or subcellular locations. In leaves, CGf detected heat-triggered cytosolic Ca2+ signals, comprised of three different signatures showing similarly rapid rates of Ca2+ influx followed by differing rates of efflux (50% durations ranging from 5 to 19 min). These heat-triggered Ca2+ signals were approximately 1.5-fold greater in magnitude than blue light-triggered signals in the same leaves. In contrast, growing pollen tubes showed two different heat-triggered responses. Exposure to heat caused tip-focused steady growth [Ca2+]cyt oscillations to shift to a pattern characteristic of a growth arrest (22%), or an almost undetectable [Ca2+]cyt (78%). Together, these contrasting examples of heat-triggered Ca2+ responses in leaves and pollen highlight the diversity of Ca2+ signals in plants, inviting speculations about their differing kinetic features and biological functions.

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Insights into the Mechanisms of Heat Priming and Thermotolerance in Tobacco Pollen

Cited by 5 publications

References 71 publications

Environmental Stress and Plants

Environmental Stress and Plants

Collective excitations of germinating pollen grains at critical points

A Ratiometric Calcium Reporter CGf Reveals Calcium Dynamics Both in the Single Cell and Whole Plant Levels Under Heat Stress

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