Insights into the mechanisms of desiccation resistance of the Patagonian PAH-degrading strain<i>Sphingobium</i>sp. 22B

Madueño, Laura; Coppotelli, Bibiana Marina; Festa, Sabrina; Alvarez, Héctor M.; Morelli, Irma Susana

doi:10.1111/jam.13742

Cited by 13 publications

(11 citation statements)

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“…Beyond the eminent biotechnological potential as bioplastics with similar physicochemical properties to petrochemical materials, while highly degradable in the environment, PHB has been demonstrated to be a critical biopolymer for microbial physiology (Kim et al 2013;Alves et al 2016;Koskimäki et al 2016;Nowroth et al 2016;Batista et al 2018). Several studies have associated PHB synthesis and degradation to positive fitness in bacteria and protection against abiotic and biotic stressors (Ayub et al 2009;Nowroth et al 2016;Madueño et al 2018;Obruca et al 2018;Alves et al 2020;Tribelli et al 2020). Herein, we present an overview of the biogenesis of PHB granules and their protective role against stressors, the stress-relieving mechanisms, and recent data on how bacteria control the cycle of PHB synthesis and degradation, opening an important window of opportunity to engineer unique microbes for bioprocesses, bioremediation of pollutants, and biofertilization of plants.…”

Section: Introductionmentioning

confidence: 99%

The protective role of PHB and its degradation products against stress situations in bacteria

Müller-Santos

Koskimäki

Alves

et al. 2020

FEMS Microbiology Reviews

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Many bacteria produce storage biopolymers that are mobilized under conditions of metabolic adaptation, for example, low nutrient availability and cellular stress. Polyhydroxyalkanoates (PHA) are often found as carbon storage in Bacteria or Archaea, and polyhydroxybutyrate (PHB) is the more frequent. Bacteria usually produce PHB upon availability of a carbon source and limitation of another essential nutrient. Therefore, it is widely believed that the function of PHB is to serve as a mobilizable carbon repository when bacteria face carbon limitation, supporting their survival. However, recent findings indicate that bacteria switch from PHB synthesis to mobilization under stress conditions such as thermal and oxidative shock. The mobilization products, 3-hydroxybutyrate and its oligomers, show a protective effect against protein aggregation and cellular damage caused by reactive oxygen species and heat shock. Thus, bacteria should have an environmental monitoring mechanism directly connected to the regulation of the PHB metabolism. Here, we review the current knowledge on PHB physiology together with a summary of recent findings on novel functions of PHB in stress resistance. Potential applications of these new functions are also presented.

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Section: Introductionmentioning

confidence: 99%

The protective role of PHB and its degradation products against stress situations in bacteria

Müller-Santos

Koskimäki

Alves

et al. 2020

FEMS Microbiology Reviews

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“…Sphingobium sp. are known to use alkanes, polycyclic aromatic hydrocarbons (PAH), polyhydroxyalkanoates like polyhydroxybutyrate (PHB) and other aromatic compounds as alternative carbon sources for growth 17 – 40 . Hence, we tested if CC4533 can utilize saturated hydrocarbons, PAH, other biohazardous aromatic compounds and PHB as carbon sources for growth.…”

Section: Resultsmentioning

confidence: 99%

“…PHB is important for the physiology of the PAH-degrading strain Sphingobium sp. 22B, isolated from semi-arid region of Patagonia (South America) as it accumulates significant amounts of this polyhydroxyalkanoate 40 . PHB has been shown to play diverse roles under different degrees of water stress: it can serve as an endogenous carbon source under low water stress and can protect cells under high water stress 40 .…”

Section: Discussionmentioning

confidence: 99%

“…Many Sphingobium strains capable of degrading PAHs and other aromatic compounds have been isolated 17 – 40 . Sphingobium yanoikuyae strain B1, previously known as Sphingomonas yanoikuyae strain B1 and Beijerinckia sp.…”

Section: Discussionmentioning

confidence: 99%

“…and Sphingobium sp. are of particular interest due to their abilities to degrade cycloalkanes and polycyclic aromatic hydrocarbons (PAH) and polyhydroxyalkanoates (PHA) and their roles in environmental bioremediation 17 – 40 . Hence, we monitored the growth of CC4533 on TP-agar medium plates containing common biohazardous chemicals that are found in our natural environment.…”

Section: Introductionmentioning

confidence: 99%

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Isolation and characterization of a novel Sphingobium yanoikuyae strain variant that uses biohazardous saturated hydrocarbons and aromatic compounds as sole carbon sources

et al. 2020

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Background: Green micro-alga, Chlamydomonas reinhardtii (a Chlorophyte), can be cultured in the laboratory heterotrophically or photo-heterotrophically in Tris-Phosphate-Acetate (TAP) medium, which contains acetate as the carbon source. Chlamydomonas can convert acetate in the TAP medium to glucose via the glyoxylate cycle, a pathway present in many microbes and higher plants. A novel bacterial strain, CC4533, was isolated from a contaminated TAP agar medium culture plate of a Chlamydomonas wild type strain. In this article, we present our research on the isolation, and biochemical and molecular characterizations of CC4533. Methods: We conducted several microbiological tests and spectrophotometric analyses to biochemically characterize CC4533. The 16S rRNA gene of CC4533 was partially sequenced for taxonomic identification. We monitored the growth of CC4533 on Tris-Phosphate (TP) agar medium (lacks a carbon source) containing different sugars, aromatic compounds and saturated hydrocarbons, to see if CC4533 can use these chemicals as the sole source of carbon. Results: CC4533 is a Gram-negative, non-enteric yellow pigmented, aerobic, mesophilic bacillus. It is alpha-hemolytic and oxidase-positive. CC4533 can ferment glucose, sucrose and lactose, is starch hydrolysis-negative, resistant to penicillin, polymyxin B and chloramphenicol. CC4533 is sensitive to neomycin. Preliminary spectrophotometric analyses indicate that CC4533 produces b-carotenes. NCBI-BLAST analyses of the partial 16S rRNA gene sequence of CC4533 show 99.55% DNA sequence identity to that of Sphingobium yanoikuyae strain PR86 and S. yanoikuyae strain NRB095. CC4533 can use cyclo-chloroalkanes, saturated hydrocarbons present in car motor oil, polyhydroxyalkanoate, and mono- and poly-cyclic aromatic compounds, as sole carbon sources for growth. Conclusions: Taxonomically, CC4533 is very closely related to the alpha-proteobacterium S. yanoikuyae, whose genome has been sequenced. Future research is needed to probe the potential of CC4533 for environmental bioremediation. Whole genome sequencing of CC4533 will confirm if it is a novel strain of S. yanoikuyae or a new Sphingobium species.

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Grazing enhanced spatial heterogeneity of soil dehydrogenase activity in arid shrublands of Patagonia, Argentina

et al. 2019

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Insights into the mechanisms of desiccation resistance of the Patagonian PAH-degrading strainSphingobiumsp. 22B

Abstract: Understanding the physiology implicated in the toleration of the PAH-degrading strain Sphingobium sp 22B to environmental desiccation may improve the bioaugmentation technologies in semi-arid hydrocarbon-contaminated soils.

Cited by 13 publications

References 58 publications

The protective role of PHB and its degradation products against stress situations in bacteria

The protective role of PHB and its degradation products against stress situations in bacteria

Isolation and characterization of a novel Sphingobium yanoikuyae strain variant that uses biohazardous saturated hydrocarbons and aromatic compounds as sole carbon sources

Grazing enhanced spatial heterogeneity of soil dehydrogenase activity in arid shrublands of Patagonia, Argentina

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