Insights into the mode of action of tannin-based feed additives in broiler chickens: looking for connections with the plasma metabolome and caecal microbiota

Brugaletta, Giorgio; Luise, Diana; Cesare, Alessandra De; Zampiga, Marco; Laghi, Luca; Trevisi, Paolo; Manfreda, Gerardo; Sirri, Federico

doi:10.1080/1828051x.2020.1842813

Cited by 10 publications

(5 citation statements)

References 65 publications

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“…For example, introduction of quebracho tannins into in vitro fermentations containing growth media with various food components and human stool samples showed increased abundances of members of the Lachnospiraceae , Ruminococcaceae (including Faecalibacterium) , Christensenellaceae , and Peptostreptococcaceae , and decreased the abundance of Parabacteriodes 34 . Similarly, feeding studies in swine and poultry show that introducing high-tannin sorghum into the feed stimulates increased abundances of members of the Lachnospiraceae , Ruminococcaceae (including Faecalibacterium ), and Peptostreptococcaceae in fecal and cecal microbiota 35 – 37 . Work in ruminants shows that condensed tannins can reduce methane production and may do so by altering the gut microbiome although the associated organisms are yet unknown 38 .…”

Section: Discussionmentioning

confidence: 99%

Genetic analysis of seed traits in Sorghum bicolor that affect the human gut microbiome

et al. 2022

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Prebiotic fibers, polyphenols and other molecular components of food crops significantly affect the composition and function of the human gut microbiome and human health. The abundance of these, frequently uncharacterized, microbiome-active components vary within individual crop species. Here, we employ high throughput in vitro fermentations of pre-digested grain using a human microbiome to identify segregating genetic loci in a food crop, sorghum, that alter the composition and function of human gut microbes. Evaluating grain produced by 294 sorghum recombinant inbreds identifies 10 loci in the sorghum genome associated with variation in the abundance of microbial taxa and/or microbial metabolites. Two loci co-localize with sorghum genes regulating the biosynthesis of condensed tannins. We validate that condensed tannins stimulate the growth of microbes associated with these two loci. Our work illustrates the potential for genetic analysis to systematically discover and characterize molecular components of food crops that influence the human gut microbiome.

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Section: Discussionmentioning

confidence: 99%

Genetic analysis of seed traits in Sorghum bicolor that affect the human gut microbiome

et al. 2022

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“…From 1 bird per replicate (i.e., 12 birds/group), selected at slaughter (42 d) according to BW close to the flock average, blood and caecal content were sampled as previously described 61 , 62 . Briefly, blood was collected, poured into 4 mL lithium-heparin vials, and centrifuged (4,000 g; 900 s; 4 °C) to get plasma.…”

Section: Methodsmentioning

confidence: 99%

A multi-omics approach to elucidate the mechanisms of action of a dietary muramidase administered to broiler chickens

Brugaletta

Cesare

Laghi

et al. 2022

Sci Rep

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A novel dietary muramidase has been shown to have positive effects on broiler chickens. However, very little is known about its mechanisms of action. The present multi-omics investigation sought to address this knowledge gap. A total of 2,340 day-old male broilers were assigned to 3 groups (12 replicates each) fed, from 0 to 42 d, a basal diet (control group—CON) or the basal diet supplemented with muramidase at 25,000 (low-dose group—MUL) or 45,000 LSU(F)/kg feed (high-dose group—MUH). MUH significantly outperformed CON in terms of cumulative feed intake (4,798 vs 4,705 g), body weight (2,906 vs 2,775 g), and feed conversion ratio (1.686 vs 1.729), while MUL exhibited intermediate performance. At caecal level, MUH showed the lowest alpha diversity, a significantly different beta diversity, a reduction in Firmicutes, and a rise in Bacteroidetes, especially compared with MUL. MUH also exhibited a considerable decrease in Clostridiaceae and an overrepresentation of Bacteroidaceae and Lactobacillaceae. At blood level, MUH had lower hypoxanthine—probably due to its drop at caecal level—histidine, and uracil, while greater pyruvate, 2-oxoglutarate, and glucose. This study sheds light on the mode of action of this muramidase and lays the groundwork for future investigations on its effects on the intestinal ecosystem and systemic metabolism of broiler chickens.

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“…In a previous study on the effects of microcapsules containing three doses of TA (500 mg/kg; 1000 mg/kg; 1500 mg/kg), respectively administered to three groups of weaned piglets, only the 1000 mg/kg GCT group showed positive regulatory effects on the intestinal microbiota [49]. Brugaletta et al [50] studied two different types of tannin supplements, A and C, added at 0.12-0.13% to broiler diets, and found that the cecal microbial diversity of the broilers in the tannin supplement C group was significantly reduced. At present, there are few studies on the effects of GCT on the intestinal microbiota of broilers, and our study only reflected the effects of 300 mg/kg GCT on the intestinal microbiota of broilers, which may be related to the dose of GCT.…”

Section: Discussionmentioning

confidence: 98%

Effects of Dietary Galla Chinensis Tannin Supplementation on Antioxidant Capacity and Intestinal Microbiota Composition in Broilers

Yuan,

Ren,

Niu

et al. 2023

Agriculture

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The current study aimed to investigate the effects of dietary Galla Chinensis tannin (GCT) supplementation on antioxidant capacity and gut microbiota composition in broilers. Two hundred eighty-eight Arbor Acres broiler chicks were divided into the CON group and the GCT group; each treatment group contained 6 replicates with 24 broiler chicks per replicate for a period of 42 days, and were fed either a basal diet or the basal diet supplemented with 300 mg/kg GCT. Results revealed that GCT supplementation significantly increased glutathione peroxidase (GSH-Px) activity (p < 0.05) and significantly decreased malondialdehyde (MAD) concentrations in serum (p < 0.05) and significantly increased GSH-Px and catalase (CAT) (p < 0.05) and significantly decreased MDA concentrations in the small intestine. In addition, GCT significantly up-regulated (p < 0.05) the gene expressions of nuclear factor erythroid 2-related factor 2 (Nrf2), heme-oxygenase 1 (HO-1), catalase (CAT), glutathione peroxidase-1 (GPX1), and NAD(P)H quinone oxidoreductase 1(NQO1). High-throughput sequencing results showed that GCT supplementation significantly increased abundances of Faecalibacterium and Megamonas (p < 0.05). These findings will contribute to our understanding of the effects of dietary Galla Chinensis tannin supplementation on antioxidant capacity and intestinal microbiota composition in broilers.

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Insights into the mode of action of tannin-based feed additives in broiler chickens: looking for connections with the plasma metabolome and caecal microbiota

Cited by 10 publications

References 65 publications

Genetic analysis of seed traits in Sorghum bicolor that affect the human gut microbiome

Genetic analysis of seed traits in Sorghum bicolor that affect the human gut microbiome

A multi-omics approach to elucidate the mechanisms of action of a dietary muramidase administered to broiler chickens

Effects of Dietary Galla Chinensis Tannin Supplementation on Antioxidant Capacity and Intestinal Microbiota Composition in Broilers

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