Insights into the Molecular Regulation of Lignin Content in Triploid Poplar Leaves

Xu, Tingting; Zhang, Shuwen; Du, Kang; Yang, Jun‐Bo; Kang, Xiangyang

doi:10.3390/ijms23094603

Cited by 18 publications

(10 citation statements)

References 44 publications

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“…Our findings indicate that the lignin content of tetraploids P. hopeiensis was significantly lower than that of their diploid counterparts. This suggests that gene dosage effects might increase the size of cells and organelles by altering gene expression, and this is usually accompanied by a decrease in cell surface area per unit volume, which might reduce lignin accumulation [ 18 ]. We also found that the cell wall thickness of tetraploids was significantly higher than that of diploids, indicating that the reduction in the lignin content in the cell wall might be compensated for by another cell wall polymer or matrix polysaccharide, such as cellulose, hemicellulose, and pectin [ 12 ].…”

Section: Discussionmentioning

confidence: 99%

SCL14 Inhibits the Functions of the NAC043–MYB61 Signaling Cascade to Reduce the Lignin Content in Autotetraploid Populus hopeiensis

Kong

Zhou

et al. 2023

IJMS

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Whole-genome duplication often results in a reduction in the lignin content in autopolyploid plants compared with their diploid counterparts. However, the regulatory mechanism underlying variation in the lignin content in autopolyploid plants remains unclear. Here, we characterize the molecular regulatory mechanism underlying variation in the lignin content after the doubling of homologous chromosomes in Populus hopeiensis. The results showed that the lignin content of autotetraploid stems was significantly lower than that of its isogenic diploid progenitor throughout development. Thirty-six differentially expressed genes involved in lignin biosynthesis were identified and characterized by RNA sequencing analysis. The expression of lignin monomer synthase genes, such as PAL, COMT, HCT, and POD, was significantly down-regulated in tetraploids compared with diploids. Moreover, 32 transcription factors, including MYB61, NAC043, and SCL14, were found to be involved in the regulatory network of lignin biosynthesis through weighted gene co-expression network analysis. We inferred that SCL14, a key repressor encoding the DELLA protein GAI in the gibberellin (GA) signaling pathway, might inhibit the NAC043–MYB61 signaling functions cascade in lignin biosynthesis, which results in a reduction in the lignin content. Our findings reveal a conserved mechanism in which GA regulates lignin synthesis after whole-genome duplication; these results have implications for manipulating lignin production.

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Section: Discussionmentioning

confidence: 99%

SCL14 Inhibits the Functions of the NAC043–MYB61 Signaling Cascade to Reduce the Lignin Content in Autotetraploid Populus hopeiensis

Kong

Zhou

et al. 2023

IJMS

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“…In this study, triploid clones showed higher averages of LL, LA, P n , and WUE , which was the same result as in other research. This may indicate that plants with an increased number of chromosomes may cause some morphological changes by influencing various physiological activities and metabolic processes [ 49 ]. However, the Pn and WUE values of the triploid were higher than those of the diploid and tetraploid; these results were the same as those of research on hybrid poplar clones [ 26 ], but in P. ussuriensis , triploid is the largest, diploid is the smallest, and tetraploid is the center [ 48 ].…”

Section: Discussionmentioning

confidence: 99%

Variations in Growth and Photosynthetic Traits of Polyploid Poplar Hybrids and Clones in Northeast China

Jiang

Cai

et al. 2022

Genes

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To evaluate differences among 19 different ploidy hybrid poplar clones grown in northeast China, 21 traits related to growth traits and photosynthetic characteristics were detected and analyzed. Abundant phenotypic variations exist among and within populations, and these variations are the basis of forest tree genetic improvements. In this research, variance analysis showed that the traits except the net photosynthesis rate among the different ploidies and all the other traits exhibited significant differences among the ploidies or clones (p < 0.01). Estimation of phenotypic coefficients of variation, genotypic coefficients of variation, and repeatability is important for selecting superior materials. The larger the value, the greater the potential for material selection improvement. The repeatability of the different traits ranged from 0.88 to 0.99. The phenotypic and genotypic coefficients of variation of all the investigated traits ranged from 6.88% to 57.40% and from 4.85% to 42.89%, respectively. Correlation analysis showed that there were significant positive correlations between tree height, diameter, and volume. Transpiration rate, intercellular carbon dioxide concentration, and stomatal conductance were significantly positively correlated with each other but negatively correlated with instantaneous water use efficiency. Growth traits were weakly correlated with photosynthetic indexes. The rank correlation coefficient showed that most of the growth indicators reached a significant correlation level among different years (0.40–0.98), except 1-year-old tree height with 4-year-old tree height and 1-year-old ground diameter with 3-year-old tree height, which indicated the potential possibility for early selection of elite clones. Principal analysis results showed that the contribution rate of the first principal component was 46.606%, and 2-year-old tree height, 2-year-old ground diameter, 3-year-old tree height, 3-year-old ground diameter, 3-year-old diameter at breast height, 3-year-old volume, 4-year-old tree height, 4-year-old ground diameter, 4-year-old diameter at breast height, and 4-year-old volume showed higher vector values than other traits. With the method of multiple-trait comprehensive evaluation to evaluate clones, SX3.1, SY3.1, and XY4.2 were selected as elite clones, and the genetic gains of height, basal diameter, diameter at breast height, and volume of selected clones ranged from 12.85% to 64.87% in the fourth growth year. The results showed fundamental information for selecting superior poplar clones, which might provide new materials for the regeneration and improvement of forests in Northeast China.

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“…The flavonoid content was expressed as mg g −1 sample. The lignin content was determined by spectrophotometry according to the method of Xu et al 27 . Acetyl lignin is produced after the phenol hydroxyl in lignin is acetylated.…”

Section: Methodsmentioning

confidence: 99%

Exogenous Brassinosteroid Enhances Zinc tolerance by activating the Phenylpropanoid Biosynthesis pathway in Citrullus lanatus L

Liu

Zhu

et al. 2023

Plant Signaling & Behavior

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Zinc (Zn) is an important element in plants, but over-accumulation of Zn is harmful. The phytohormone brassinosteroids (BRs) play a key role in regulating plant growth, development, and response to stress. However, the role of BRs in watermelon ( Citrullus lanatus L.) under Zn stress, one of the most important horticultural crops, remains largely unknown. In this study, we revealed that 24-epibrassinolide (EBR), a bioactive BR enhanced Zn tolerance in watermelon plants, which was related to the EBR-induced increase in the fresh weight, chlorophyll content, and net photosynthetic rate (Pn) and decrease in the content of hydrogen peroxide (H 2 O 2 ), malondialdehyde (MDA), and Zn in watermelon leaves. Through RNA deep sequencing (RNA-seq), 350 different expressed genes (DEG) were found to be involved in the response to Zn stress after EBR treatment, including 175 up-regulated DEGs and 175 down-regulated DEGs. The up-regulated DEGs were significantly enriched in ‘phenylpropanoid biosynthesis’ pathway (map00940) using KEGG enrichment analysis. The gene expression levels of PAL , 4CL , CCR , and CCoAOMT , key genes involved in phenylpropanoid pathway, were significantly induced after EBR treatment. In addition, compared with Zn stress alone, EBR treatment significantly promoted the activities of PAL, 4CL, and POD by 30.90%, 20.69%, and 47.28%, respectively, and increased the content of total phenolic compounds, total flavonoids, and lignin by 23.02%, 40.37%, and 29.26%, respectively. The present research indicates that EBR plays an active role in strengthening Zn tolerance, thus providing new insights into the mechanism of BRs enhancing heavy metal tolerance.

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Insights into the Molecular Regulation of Lignin Content in Triploid Poplar Leaves

Cited by 18 publications

References 44 publications

SCL14 Inhibits the Functions of the NAC043–MYB61 Signaling Cascade to Reduce the Lignin Content in Autotetraploid Populus hopeiensis

SCL14 Inhibits the Functions of the NAC043–MYB61 Signaling Cascade to Reduce the Lignin Content in Autotetraploid Populus hopeiensis

Variations in Growth and Photosynthetic Traits of Polyploid Poplar Hybrids and Clones in Northeast China

Exogenous Brassinosteroid Enhances Zinc tolerance by activating the Phenylpropanoid Biosynthesis pathway in Citrullus lanatus L

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