Insights into the potential mechanism underlying liver dysfunction in male albino rat exposed to gasoline fumes

Owagboriaye, Folarin; Aina, Sulaimon; Oladunjoye, Rasheed; Salisu, Titilola; Adenekan, Adedamola; Dedeke, G. A.

doi:10.1080/2314808x.2021.1992118

Cited by 6 publications

(5 citation statements)

References 46 publications

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“…These reductions could be attributed to the increased lipid peroxidation since Na+ /K+ -ATPase is lipid dependent and has a high affinity for the SH group and Mg2 + -ATPase activity is sensitive to lipid peroxidation [66] . In addition, Miltonprabu et al [66] noted that Na+ /K+ -ATPase inhibition could lead to a decrease in sodium efflux that disrupts membrane permeability, a condition that may lead to the leakage of Ca2 + ions into the cytoplasm and consequently reduce the Ca2 +ATPase activity in the membrane as previously suggested [42] , [43] .…”

Section: Discussionmentioning

confidence: 91%

“…The level of reactive oxygen species (ROS) in the brain was determined according to the protocol described in P é rez-Severiano et al [41] and as modified [42] , [43] . The protocol is based on the incubation of 100 μl of brain aliquots with 5 μl of 2′,7′-dichloro-dihydro fluorescein diacetate (DCFH-DA) at 37 °C for 60 min.…”

Section: Methodsmentioning

confidence: 99%

“…Activities of Ca 2+ ATPase, Na + /K + ATPase and Mg 2+ ATPase in the brain homogenates of the rats were determined according to the protocols described in Afolabi et al [44] ; Dedeke et al [45] and as modified in Owagboriaye et al [42] , [43] . The assay involves the estimation of the amount of phosphorus liberated from the incubation mixture containing the homogenate, 5 mM of ATP, 60 mM of NaCl, 2 mM of MgCl 2 , 20 mM of KCl, 2 Mm of CaCl 2 and protein enzyme.…”

Section: Methodsmentioning

confidence: 99%

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Outcome of the first survey of atrazine in drinking water from Ijebu-North, South-West, Nigeria: Human health risk and neurotoxicological implications

Owagboriaye

Oladunjoye²,

Aina³

et al. 2022

Toxicology Reports

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Section: Discussionmentioning

confidence: 91%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Outcome of the first survey of atrazine in drinking water from Ijebu-North, South-West, Nigeria: Human health risk and neurotoxicological implications

Owagboriaye

Oladunjoye²,

Aina³

et al. 2022

Toxicology Reports

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“…However, previous studies reported gasoline‐induced hepatotoxicity, which manifested as elevated liver oxidative stress, inflammatory, and fibrotic markers along with decreased antioxidants in male albino rats after 10 weeks of exposure to gasoline (Abdrabouh, 2019). In addition, augmented reactive oxygen species (ROS) production as well as alteration in the activity of the liver enzymes, mixed function oxidase, and membrane bound ATPase were noted in 10‐week GE rats (Owagboriaye et al, 2021). Therefore, the unchanged expression of inflammatory cytokines in the liver of GE mice compared to CONs that we report in our study could be mainly attributed to the shorter gasoline exposure (1.5 h a day and thus for 5 days per week over 7 consecutive weeks) that was sufficient to induce a well‐established inflammation in the lungs in contrast to a mild inflammation in the liver.…”

Section: Discussionmentioning

confidence: 99%

Gasoline fume inhalation induces apoptosis, inflammation, and favors Th2 polarization in C57BL/6 mice

Nour‐Eldine

Sayyed

Harhous

et al. 2022

J of Applied Toxicology

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Gasoline exposure has been widely reported in the literature as being toxic to human health. However, the exact underlying molecular mechanisms triggered by its inhalation have not been thoroughly investigated. We herein present a model of subchronic, static gasoline vapor inhalation in adult female C57BL/6 mice. Animals were exposed daily to either gasoline vapors (0.86 g/animal/90 min) or ambient air for 5 days/week over 7 consecutive weeks. At the end of the study period, toxic and molecular mechanisms underlying the inflammatory, oxidative, and apoptotic effects triggered by gasoline vapors, were examined in the lungs and liver of gasolineexposed (GE) mice.Static gasoline exposure induced a significant increase (+21%) in lungs/body weight (BW) ratio in GE versus control (CON) mice along with a pulmonary inflammation attested by histological staining. The latter was consistent with increases in the transcript levels of proinflammatory cytokines [Interleukins (ILs) 4 and 6], respectively by $ 6-and 4-fold in the lungs of GE mice compared to CON. Interestingly, IL-10 expression was also increased by $ 10-fold in the lungs of GE mice suggesting an attempt to counterbalance the established inflammation. Moreover, the pulmonary expression of IL-12 and TNF-α was downregulated by 2-and 4-fold, respectively, suggesting the skewing toward Th2 phenotype. Additionally, GE mice showed a significant upregulation in Bax/Bcl-2 ratio, caspases 3, 8, and 9 with no change in JNK expression in the lungs, suggesting the activation of both intrinsic and extrinsic apoptotic pathways. Static gasoline exposure over seven consecutive weeks had a minor hepatic portal inflammation attested by H&E staining along with an increase in the hepatic expression of the mitochondrial complexes in GE mice. Therefore, tissue damage biomarkers highlight the health risks associated with vapor exposure and may present potential therapeutic targets for recovery from gasoline intoxication.

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“…The level of ROS in the testicular tissue was determined by incubating 100 μL of an aliquot from testicular homogenate with 5 μL 2′,7′-dichloro-dihydro fluorescein diacetate (DCFH-DA) at 37°C for 60 min as previously described in Pérez-Severiano et al (2004) and Owagboriaye et al (2021) , Owagboriaye et al (2022) . The thiobarbituric acid reactive substance (TBARS) assay described in Okhawa et al (1979) , which involves the measurement of malondialdehyde (MDA) was employed for the determination of lipid peroxidation.…”

Section: Toxicity Studymentioning

confidence: 99%

First report on atrazine monitoring in drinking water from Ijebu-North, South-West Nigeria: Human health risk evaluation and reproductive toxicity studies

et al. 2022

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There are no available data on the level of atrazine in drinking water from rural agricultural areas in Nigeria and its potential health implications. Here, we measured atrazine residue in 69 hand-dug wells (HDW), 40 boreholes (BH), and four major streams from the six communities (Ago-Iwoye, Ijebu-Igbo, Oru, Awa, Ilaporu, and Mamu) in Ijebu North Local Government Area, Southwest Nigeria. Values of atrazine obtained were further used for the evaluation of non-carcinogenic risk associated with ingestion and dermal contact in adults and children as well as reproductive toxicity evaluation. A total of 41 HDW, 22 BH, and the four streams showed varying concentrations of atrazine, which was higher in HDW than BH and stream. Ago-Iwoye recorded the highest concentration of 0.08 mg/L in its HDW while the lowest concentration of 0.01 mg/L was recorded in HDW from Oru. Although the Hazard Index (HI) values associated with ingestion and dermal contact for children were higher than in adults, the values were below the acceptable limit for all the communities. Significant (p < 0.05) alterations in the oxidative stress parameters, reproductive hormones, sperm parameters, and mild testicular lesions were only observed in rats exposed to atrazine at 0.08 mg/L compared to control. But atrazine at 0.01, 0.03, and 0.04 mg/L triggered a defence mechanism capable of protecting the structural integrity of the testes and preventing reproductive dysfunction.

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Insights into the potential mechanism underlying liver dysfunction in male albino rat exposed to gasoline fumes

Cited by 6 publications

References 46 publications

Outcome of the first survey of atrazine in drinking water from Ijebu-North, South-West, Nigeria: Human health risk and neurotoxicological implications

Outcome of the first survey of atrazine in drinking water from Ijebu-North, South-West, Nigeria: Human health risk and neurotoxicological implications

Gasoline fume inhalation induces apoptosis, inflammation, and favors Th2 polarization in C57BL/6 mice

First report on atrazine monitoring in drinking water from Ijebu-North, South-West Nigeria: Human health risk evaluation and reproductive toxicity studies

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